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Citations for Millipore Sigma :
501 - 550 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Triplicate reactions were started at RT in PCR tubes by addition of an NAD+-mixture resulting to final concentrations of 24.5 µM NAD+ (Sigma, N3014) and 0.5 µM of biotinylated NAD+ (Trevigen ...
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bioRxiv - Molecular Biology 2023Quote: 10% of the RT reaction was used as the template in 25 μl PCR reactions containing 1.25U of JumpStart Taq Polymerase (Sigma D9307), 1×PCR buffer (Sigma P2192) ...
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bioRxiv - Developmental Biology 2022Quote: ... A probe for Nr2f2 was prepared by amplifying a cDNA fragment by RT-PCR (primers provided below, Table 4) from total RNA isolated from E9.5 embryos with Tri Reagent (Sigma #93289) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The mRNA expression levels of PIWIL1 and Actin were examined by semiquantitative RT-PCR from total RNA using TriZol Reagent (Sigma) 72 h after siRNA transfection.
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bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with SYBR Green Quantitative RT-qPCR Kit (Sigma-Aldrich; Cat. No. QR0100) using GAPDH as a housekeeping gene ...
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bioRxiv - Molecular Biology 2023Quote: ... or MMLV-RT (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Ant-miR-218 or Ant-scrambled was dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3nM and infused with a total volume of 0.5μl of Ant-miR-218 or Ant-scrambled over a 7-min period as in(31 ...
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bioRxiv - Neuroscience 2019Quote: ... Ant-miR-218 or Ant-scrambled were dissolved in sterile PBS (Sigma-Aldrich, Oakville, ON, Canada) at a final concentration of 0.3 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 mMTris-HCl in 2x SSC) supplemented with 1 μl of 3.7 mg/ml glucose oxidase (Sigma-Aldrich) and 1 μl of 5 mg/ml Catalase (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were incubated for pre-hybridization in wash buffer (2x saline-sodium citrate (SSC) with deionized formamide (Sigma) 10% ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in hybridization buffer (50% Formamide, 4x SSC pH 4.5, 1% SDS, 50μg/ml heparin (Sigma), 10μg/ml tRNA from baker’s yeast (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were incubated for pre-hybridization in wash buffer (2x saline-sodium citrate (SSC) with deionized formamide (Sigma) 10% ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was discarded and the pellet was resuspended in a small volume of SSC cocktail (3xSSC [Sigma; S0902] ...
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bioRxiv - Cell Biology 2022Quote: ... dishes were washed with wash buffer (1x saline-sodium citrate, SSC, Sigma-Aldrich, in 10% deionized formamide, Ambion) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were washed with the RNAscope wash buffer and incubated with 5X SSC (Sigma-Aldrich, S6639-1L) overnight at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed with stringent buffer (1:1, formamide:4X SSC buffer; Thermo Fisher #AM9342; Sigma-Aldrich #S6639) at 37°C twice for 25 minutes each ...
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bioRxiv - Developmental Biology 2023Quote: ... SSCs were seeded on STO feeders which were prepared 1 day before on Millicell EZ SLIDE (PEZGS0816, Millipore). SSCs were treated with 4-OHT at 0.1µM or RA at 1 µM or vehicle for the indicated time ...
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bioRxiv - Developmental Biology 2022Quote: ... The dehydrated samples were then incubated in a 1:1 mixture of propylene oxide and absolute ethanol for five minutes at RT followed by a two times 10 minutes incubation in pure propylene oxide (Sigma-Aldrich, Merck, Darmstadt, Germany), and a 30 minutes incubation in a 1:1 mixture of propylene oxide and Spurr resin (Low Viscosity Spurr Kit ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were purified using a PCR cleanup kit (Sigma) and quantified using a Qubit 2.0 fluorometer (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... using RedExtract-n-Amp tissue PCR kit (Sigma-Aldrich). The G2 progeny of genome-edited males were then raised until homozygous stocks were established ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... Polymerase chain reaction (PCR) samples were prepared using KAPA2G Fast PCR Kit (Sigma St. Louis, MO) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified using the GenElute PCR purification kit (Sigma-Aldrich, St. Louis, MO). The indexing PCR was carried out to attach the dual indices (Appendix D6.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCR reactions were column purified (High Pure PCR Product Purification kit, cat#11732668001, Sigma Aldrich). 2 ng/μl of purified PCR products were injected into either N2 or SM507 strains along with 100 ng/μl of pRF4 plasmid as a selection marker to create transgenic lines with extrachromosomal arrays.
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bioRxiv - Microbiology 2022Quote: ... followed by PCR product purification using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, U.S.A). All sequencing throughout was performed using the Macrogen sequencing service (Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the GenElute PCR Clean-Up kit (Sigma-Aldrich #NA1020), digested using DpnI (Promega #R6231) ...
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bioRxiv - Cancer Biology 2020Quote: ... Gel-electrophoresis was used for the detection of PCR products (MYC 630 bp, Neu 386 bp, rtTA 380 bp) on 1,5% agarose (Sigma, A9539-500G) gel with Ethidium bromide solution (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed utilizing the S-GAG set of primers and probe (Cline et al., 2005)(Sigma-Aldrich, Burlington, MA) with a 1-step Master mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... TUNEL assay was performed with reaction solutions from ApopTag Red In situ Apoptosis detection kit (Millipore) according to the manufacturer recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... TUNEL assay was conducted with ApopTag Fluorescin Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA) on retinal cryosections according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2021Quote: Heterodimers were detected using the Duolink II in situ proximity ligation assay (PLA) detection Kit (Sigma) and following the instructions of the supplier ...
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bioRxiv - Genetics 2021Quote: ... The TUNEL assay was performed with the ApopTag Red In Situ Apoptosis Detection Kit (Millipore, S7165). Dissected larvae were fixed in 4% paraformaldehyde ...
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were stained using ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) according to manufacturers instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma contamination was evaluated routinely during cell culture using a VenorGeM Mycoplasma Detection Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... Cells were fixed in citrate- acetone-formaldehyde and stained using Alkaline Phosphatase Detection Kit (Sigma, SCR004). The rest of the cells were lysed in RIPA buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... following the detection of EP catalytic ability by using the trypsin activity kit (MAK290, Sigma-Aldrich), and cross-linking by 0.05% glutaraldehyde for complex stabilization ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were prepared and stained using ApopTag® Red In Situ Apoptosis Detection Kit (Merk Millipore) according to manufacturer’s instructions and imaged on a Zeiss Axio Observer wide-field fluorescence microscope.
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bioRxiv - Immunology 2019Quote: ... before subsequent TUNEL staining for apoptosis using ApopTag® Red In Situ Apoptosis Detection Kit (Millipore).
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bioRxiv - Cancer Biology 2020Quote: EdU detection was done according using the EdU-Click Chemistry 488 kit (Sigma-Aldrich, BCK-EDU488) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cell lines were tested twice a year for Mycoplasma contamination using the LookOut Detection Kit (Sigma), and all tests were negative.
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bioRxiv - Cell Biology 2020Quote: Alcaline phosphatase activity was determined using a detection kit according to manufacturer recommendations (Sigma-Aldrich, SCR004).
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bioRxiv - Cell Biology 2021Quote: Apoptosis in kidneys was detected using the In Situ Cell Death Detection Kit (Sigma Aldrich/Roche). Briefly ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Brains were then incubated with TUNEL reagent (In Situ Cell Death Detection Kit, TMR red, Sigma) for 14-16 hours at 37°C in dark humid chamber ...