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Citations for Millipore Sigma :
101 - 150 of 2891 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... H3K4me3 (Millipore, 17-614) and H3K9me3 (Millipore ...
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bioRxiv - Genetics 2019Quote: ... MeRIP (17-701, Millipore) was performed according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... EZH2 (17-662, Millipore), Histone H3 (ab1791 ...
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bioRxiv - Cancer Biology 2021Quote: ... EED (Millipore 17-10034), rabbit IgG (CST 2729) ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520, Millipore) with 5 μg IgG or anti-Wdr82 antibody in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... H3K27me1 (17-643/Millipore) and H1 (AS111801/Agrisera ...
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bioRxiv - Plant Biology 2022Quote: ... 17-b-oestradiol (Sigma), a synthetic derivative of oestradiol ...
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bioRxiv - Plant Biology 2023Quote: ES9-17 (Sigma Aldrich) was dissolved in DMSO to create a 30 mM stock solution ...
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bioRxiv - Cancer Biology 2020Quote: ... After probe hybridization increasingly stringent washing steps were applied in saline-sodium citrate (SSC) buffer (Sigma, SSC 20x buffer concentrate S6639-1L) at various concentrations and temperatures ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were then rinsed in 2X-SSC (1X SSC: 150mM sodium chloride, 15mM sodium citrate) 3x and incubated for >24 hrs in 50% formamide (Sigma, catalog # 47671) / 2X-SSC (pH 7.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... we used these internal lipid standards: triglyceride (TG 15:0/15:0/15:0 and TG 17:0/17:0/17:0, Sigma-Aldrich), lysophosphatidylcholines (LPC ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed twice with 2x SSC (Sigma-Aldrich) for 5 min then permeabilized with 0.2 M HCl + 0.7% Triton X-100 for 10 – 15 min at RT ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
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bioRxiv - Genomics 2019Quote: ... was used along with 0.36 μl of 50 μM custom-designed SYNE2 short and long isoform primer and 0.5 μl of 10 μM probe set (Table 1, obtained from Sigma-Aldrich) and 1 μl of the primer limited cyclophilin A (PPIA ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). ELISAs were performed as per the manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Overlapping PCR primers encoding the gRNA sequences were ordered from Sigma-Aldrich and Eurofins Genomics ...
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Genetics 2020Quote: ... anti-H3K27me3 (Millipore 17-622) and anti-H3K4me3 (Active Motif 39159) ...
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bioRxiv - Genetics 2020Quote: ... anti-H3K27me3 (Millipore 17-622) and anti-H3K4me3 (Active Motif 39159).
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bioRxiv - Plant Biology 2019Quote: ... 17 μg/mL luminol (Sigma), and chitin (NaCoSy ...
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bioRxiv - Microbiology 2021Quote: For MeRIP (#17-10499, Millipore), 5 μg of fragmented mRNA was incubated with 5 μg of anti-m6A antibody or anti-IgG antibody (negative control ...
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bioRxiv - Genomics 2022Quote: ... anti-H3K4me3 (17-614, Millipore) and anti-H3K27ac (ab4729 ...
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bioRxiv - Genomics 2023Quote: ... anti-H3K27me1 (Millipore 17-643), anti-H4K20me1 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Monoheptadecanoin (17:0 monoacylglycerol) (Sigma) and Tetracosane (C24 alkane ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Developmental Biology 2020Quote: ... 2x SSC and 10% (w/v) dextran sulfate (Sigma, D8906) in a humid chamber at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... respectively: 75% probe wash buffer + 25% 5x SSC (Sigma Aldrich), 50% probe was buffer + 50% 5x SSC ...
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bioRxiv - Systems Biology 2023Quote: ... slides have been washed with 0.1X SSC buffer (Sigma S6639), and ddH2O ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed with 2x SSC (Sigma-Aldrich, #335266) and incubated in a buffer of 2x SSC ...
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bioRxiv - Microbiology 2021Quote: Colonies that produced bands of the expected size with both primer sets were inoculated into 1 ml of LB containing 1% arabinose (Sigma-Aldrich) for 1 hour at 30°C to induce I-Sce expression to linearize the inserted KanSacB cassette ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Cancer Biology 2023Quote: miR-6883 mimic was purchased from Sigma-Aldrich (HMI2616). This double-stranded RNA molecule mimics endogenous mature miRNA when introduced into cells.
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were rehydrated with 10% formamide in 2X SSC (Sigma Aldrich), twice for 5 minutes at RT ...
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bioRxiv - Systems Biology 2020Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP). The hybridization was performed at 37°C for 24-72 hours in a humid chamber ...
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bioRxiv - Systems Biology 2020Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP) was hybridized at 37°C for 48-96 hours in a humid chamber ...
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bioRxiv - Systems Biology 2021Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP). The hybridization was performed at 37°C for 3 days in a humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then covered with 5X SSC (Sigma-Aldrich, S6639-1L) buffer and left overnight until the morning ...
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bioRxiv - Cancer Biology 2022Quote: ... each well was washed with 100 μL 0.1×SSC (Sigma-Aldrich), and added with 75 μL reverse transcription Master Mix (from the Visium Reagent Kit ...
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bioRxiv - Neuroscience 2022Quote: ... the coated slide was washed 5 times with SSC 2X (Sigma) followed by 2 washes with dH2O ...
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bioRxiv - Cancer Biology 2021Quote: ... Metaphase slides in coplin jar containing 2X SSC buffer (Sigma-S6639) were equilibrated at room temperature for 10 minutes ...