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Citations for Millipore Sigma :
601 - 650 of 2891 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the sample was washed 4 times with 2X SSC and fluorophores were cleaved off by reducing the thiol bond with 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma) in 2X SSC during two washes of 10 minutes at 22 °C ...
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bioRxiv - Developmental Biology 2021Quote: Tissues were moved to GLOX buffer (0.4% Glucose, 1% Tris, and 10% SSC) containing 50 ng/ml DAPI (Sigma, D9542) for 10 min followed by GLOX buffer wash ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were washed with blocking buffer (4X-SSC/0.1% Triton X-100/4% BSA (Bovine serum albumin; Sigma, catalog #A7906)) at RT for 30 mins ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed in containers containing large volumes and under agitation (400 rpm) as following: 100 mL of preheated buffer 1 (2X SSC and 0.1X SDS (Sigma 71736); 50°C ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
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bioRxiv - Immunology 2021Quote: ... stained with the Microscopy Hemacolor-Rapid staining set (1.11674.0001 Sigma) according to the manufacturer’s protocol and imaged on a Nikon Ti2-E inverted microscope.
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bioRxiv - Microbiology 2020Quote: 0.2 μg proteomics dynamic range standard set (UPS2, Sigma-Aldrich) was added as spike-in to 50 μl protein samples of each fraction ...
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bioRxiv - Genomics 2021Quote: ... Tissue was homogenized in the grinder set (D9063, Sigma-Aldrich) by slowly douncing 15 times with pestle A and 25 times with pestle B ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 µl protease inhibitor cocktail set (EMD Millipore 539134) in a total volume of 1 ml ...
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bioRxiv - Neuroscience 2021Quote: The odor stimulus set consisted of 2-hexanone (Sigma, 02473), octanal (Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... using a glass dounce tissue grinder set (#D9063; Millipore Sigma: 20 times with pestle A and 20 times with pestle B)42 ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.4) with Protease Inhibitor Cocktail Set III (EMD Millipore). Lysates were sonicated in a water bath sonicator (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... and the On-Column DNase I Digestion set (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... or the On-Column DNase I Digestion set (Sigma-Aldrich). Reverse transcription was performed with the SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and treated with DNase (Sigma, On-Column DNaseI treatment set). RNA integrity was quality checked using the Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Genomics 2023Quote: ... using a glass dounce tissue grinder set (#D9063; Millipore Sigma; 20 times with pestle A and 20 times with pestle B) ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphatase inhibitor cocktail set II (Millipore Sigma, 1:100 dilution) and 1 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... using a glass Dounce tissue grinder set (#D9063; Millipore Sigma: 20 times with pestle A and 10 times with pestle B) ...
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bioRxiv - Cell Biology 2024Quote: ... and 1:100 Phosphatase Inhibitor Cocktail Set II (Sigma-Aldrich)) per 10 mg of tissue ...
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bioRxiv - Physiology 2020Quote: ... Primers were designed using both Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) and Primer-BLAST (NCBI, https://www.ncbi.nlm.nih.gov/tools/primer-blast/) and were purchased from Sigma-Aldrich. All primer sequence information is described in Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Human ITGB1 (encoding integrin β1) primer #1 (H_ITGB1_2) and ITGB3 (encoding integrin β3) primer #1 (H_ITGB3_1) were purchased from Sigma-Aldrich.
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bioRxiv - Developmental Biology 2023Quote: ... The primers for the qPCR reaction were purchased from “KiCqStart® SYBR® Green Primers” (Sigma-Aldrich) and the gene gadph was chosen as a control.
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bioRxiv - Neuroscience 2021Quote: ... 350μl of RNAzol RT (Sigma-Aldrich) were added to the samples and lysis was performed with TissueLyser II (Qiagen ...
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bioRxiv - Immunology 2022Quote: Chromatin immunoprecipitation assays were performed with MDMs using Chip assay kit (Millipore, #17-295) and according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The EdU Cell Proliferation Assay Kit (EdU-594, EMD Millipore Corp, USA, 17-10527) was used to measure the proliferation rate in cells ...
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bioRxiv - Physiology 2020Quote: The EdU Cell Proliferation Assay Kit (EdU-594, EMD Millipore Corp, USA, 17-10527) was used to measure cell proliferation ...
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bioRxiv - Neuroscience 2021Quote: ... 17 div cultured neurons were incubated with TrkB-Fc (1 µg/mL, T8694-Sigma) a BDNF scavenger suppressing BDNF binding to TrkB (Sakuragi et al ...
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bioRxiv - Plant Biology 2021Quote: ... the water was replaced by a solution containing 17 μg/mL luminol (Sigma Aldrich), 20 μg/mL horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ChIP assay was conducted according to the manufacturer protocol (Millipore Sigma, cat # 17-395) as previously described89,90 ...
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bioRxiv - Microbiology 2022Quote: Human 293T/17 (ATCC No.: CRL-11268) cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... ChIP was performed according to the instructions for Magna ChIP kit (Millipore #17-10085) using 5 μg of anti-FOXO1 antibody (Abcam #ab39670 ...
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bioRxiv - Plant Biology 2021Quote: ... embryos were mounted on glass slides in chloral hydrated (Sigma-Aldrich 302-17-0). Embryos with fluorescent reporters were imaged using Renaissance staining SR2200 as previously described (Smit et al ...
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bioRxiv - Cancer Biology 2020Quote: ... so animals were administered with 8 μg/ml 17-beta estradiol (Sigma-Aldrich, #E2758) in drinking water at least 4 days previously to implantation until the end of the experiment ...
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bioRxiv - Immunology 2020Quote: ... A549 cells were infected with GFP-LC3 lentivirus (17-10193; Millipore, Bedford, MA, USA) according to manufacturer instruction ...
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bioRxiv - Microbiology 2022Quote: ... and purified from bacterial lysates on glutathione agarose beads (Sigma, cat. 17-0756-01). Equal amounts of GST or GST-Trim69 proteins were then bound to 10 μg of pure porcine brain tubulin (purchased from Euromedex ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 2 mL of 17% dextran (MW 60,000; Sigma 31397) and centrifuged for 15 min at 4,122 g to separate the parenchymal cells from the vasculature ...
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bioRxiv - Cancer Biology 2020Quote: ... Immune cells were purified using a 70/30 Percoll (Sigma Aldrich, 17-0891-02) gradient spun for 30 minutes at 500G ...
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bioRxiv - Neuroscience 2022Quote: ... and 15 μL of Protein-G Sepharose (Millipore/Cytiva Cat#GE-17-0618-05), washed three times in ice-cold wash buffer (0.1% Triton X-100 in 150 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... A Magna MeRIP™ m1A Kit (Merck Millipore, MA, USA; Cat. No. 17–10499) was used to perform immunoprecipitation (IP ...
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bioRxiv - Microbiology 2023Quote: Human 293T/17 (ATCC No.: CRL-11268) cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Plant Biology 2023Quote: ... Esculin: A 100 μl drop of 8 mg/ml esculin (66778-17-4, Sigma) (Esculin was dissolved in distilled water with 0.2% DMSO and 0.4% absolute ethyl alcohol ...
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bioRxiv - Plant Biology 2023Quote: ... All the buffers for ChIP were from ChIP assay kit (Millipore, Cat. 17-295). Ground samples were placed into 1.5 mL microtube with nuclei isolation buffer for 15 min and then centrifuged at 13,000 rpm for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore, 17-10060), or control antibodies against polII or IgG ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were arrested for 17-20 h with 2.5 mM thymidine (Sigma-Aldrich) with a 9 h release interval ...
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bioRxiv - Cell Biology 2023Quote: ... The assay was performed using the Rap1 Activation Assay Kit (17-321 Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: RIP was performed using a Magnetic RIP RNA-Binding Protein Immunoprecipitation (Millipore, 17-700) by the manufacturer’s guidelines ...
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bioRxiv - Systems Biology 2021Quote: ... and miR-290-295_KO cluster mESC lines (129/Ola background) were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma-Aldrich), containing 15% fetal bovine serum (FBS ...