Labshake search
Citations for Millipore Sigma :
451 - 500 of 2520 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... DNA primers were purchased from Sigma. Synthesis of N-acetyl-1-seleno-β-D-glucosamine (SeGlcNAc ...
-
bioRxiv - Cancer Biology 2024Quote: Custom primers were acquired from Sigma; sequences are available in Supplementary Table 2 ...
-
bioRxiv - Developmental Biology 2020Quote: ... embryos were resuspended in 100 μL anti-fade buffer (0.4 % glucose, 10 μM Tris-HCl pH8, 2x SSC) with 1 μL catalase (Sigma-Aldrich) and 1 μL glucose oxidase (3.7 mg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... Hybridisation was performed by incubating coverslips with 100 - 250 nM probes diluted in hybridisation buffer (2X SSC buffer, 10% Dextran sulfate (Sigma), 10% Formamide ...
-
bioRxiv - Biophysics 2021Quote: ... Thereafter, the cells were washed twice with washing buffer (25% formamide, 2X SSC) containing DAPI (1 mg/mL, Sigma Aldrich), for 30 minutes at 30°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Fixed ovaries were washed twice in PBST for 10 minutes and incubated with a final probe concentration of 1nM/probe in 250μL HYBEC solution (2x SSC, 15% ethylene carbonate (Sigma Aldrich), 1mM EDTA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 16806-25] in 10 mM Tris-HCl [pH 8.0], 2× saline sodium citrate [SSC], glucose oxidase [37 µg/mL, Sigma-Aldrich, G2133-10KU] ...
-
bioRxiv - Neuroscience 2022Quote: ... the sample was washed 4 times with 2X SSC and fluorophores were cleaved off by reducing the thiol bond with 50 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma) in 2X SSC during two washes of 10 minutes at 22 °C ...
-
bioRxiv - Developmental Biology 2021Quote: Tissues were moved to GLOX buffer (0.4% Glucose, 1% Tris, and 10% SSC) containing 50 ng/ml DAPI (Sigma, D9542) for 10 min followed by GLOX buffer wash ...
-
bioRxiv - Genomics 2021Quote: ... Coverslips were washed with blocking buffer (4X-SSC/0.1% Triton X-100/4% BSA (Bovine serum albumin; Sigma, catalog #A7906)) at RT for 30 mins ...
-
bioRxiv - Systems Biology 2023Quote: ... Slides were washed in containers containing large volumes and under agitation (400 rpm) as following: 100 mL of preheated buffer 1 (2X SSC and 0.1X SDS (Sigma 71736); 50°C ...
-
bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
-
bioRxiv - Neuroscience 2023Quote: ... the fluorescent dyes were removed by flowing 2 mL of the cleavage buffer containing 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; 646547, Sigma) with a 15-min incubation time in the flow chamber to cleave the disulfide bond linking the dyes to the readout probes ...
-
bioRxiv - Immunology 2021Quote: ... stained with the Microscopy Hemacolor-Rapid staining set (1.11674.0001 Sigma) according to the manufacturer’s protocol and imaged on a Nikon Ti2-E inverted microscope.
-
bioRxiv - Microbiology 2020Quote: 0.2 μg proteomics dynamic range standard set (UPS2, Sigma-Aldrich) was added as spike-in to 50 μl protein samples of each fraction ...
-
bioRxiv - Genomics 2021Quote: ... Tissue was homogenized in the grinder set (D9063, Sigma-Aldrich) by slowly douncing 15 times with pestle A and 25 times with pestle B ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 5 µl protease inhibitor cocktail set (EMD Millipore 539134) in a total volume of 1 ml ...
-
bioRxiv - Neuroscience 2021Quote: The odor stimulus set consisted of 2-hexanone (Sigma, 02473), octanal (Aldrich ...
-
bioRxiv - Neuroscience 2019Quote: ... using a glass dounce tissue grinder set (#D9063; Millipore Sigma: 20 times with pestle A and 20 times with pestle B)42 ...
-
bioRxiv - Molecular Biology 2022Quote: ... pH 7.4) with Protease Inhibitor Cocktail Set III (EMD Millipore). Lysates were sonicated in a water bath sonicator (Diagenode ...
-
bioRxiv - Developmental Biology 2019Quote: ... and the On-Column DNase I Digestion set (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... or the On-Column DNase I Digestion set (Sigma-Aldrich). Reverse transcription was performed with the SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: ... and treated with DNase (Sigma, On-Column DNaseI treatment set). RNA integrity was quality checked using the Bioanalyzer 2100 (Agilent) ...
-
bioRxiv - Genomics 2023Quote: ... using a glass dounce tissue grinder set (#D9063; Millipore Sigma; 20 times with pestle A and 20 times with pestle B) ...
-
bioRxiv - Molecular Biology 2023Quote: ... phosphatase inhibitor cocktail set II (Millipore Sigma, 1:100 dilution) and 1 mM DTT ...
-
bioRxiv - Molecular Biology 2024Quote: ... using a glass Dounce tissue grinder set (#D9063; Millipore Sigma: 20 times with pestle A and 10 times with pestle B) ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1:100 Phosphatase Inhibitor Cocktail Set II (Sigma-Aldrich)) per 10 mg of tissue ...
-
bioRxiv - Physiology 2020Quote: ... Primers were designed using both Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) and Primer-BLAST (NCBI, https://www.ncbi.nlm.nih.gov/tools/primer-blast/) and were purchased from Sigma-Aldrich. All primer sequence information is described in Table 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Human ITGB1 (encoding integrin β1) primer #1 (H_ITGB1_2) and ITGB3 (encoding integrin β3) primer #1 (H_ITGB3_1) were purchased from Sigma-Aldrich.
-
bioRxiv - Developmental Biology 2023Quote: ... The primers for the qPCR reaction were purchased from “KiCqStart® SYBR® Green Primers” (Sigma-Aldrich) and the gene gadph was chosen as a control.
-
bioRxiv - Neuroscience 2021Quote: ... 350μl of RNAzol RT (Sigma-Aldrich) were added to the samples and lysis was performed with TissueLyser II (Qiagen ...
-
bioRxiv - Systems Biology 2021Quote: ... and miR-290-295_KO cluster mESC lines (129/Ola background) were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma-Aldrich), containing 15% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2019Quote: ... Eighteen microliters of high-titer lentivirus expressing miR-382 mixed with 2 μL 0.2% Evans blue dye (Sigma-Aldrich) in PBS was injected in the third mammary gland using a 34-gauge needle (Hamilton) ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos are rinsed in HB-buffer (HB+ without torula RNA and heparin) supplemented with saline-sodium citrate buffer (SSC, Sigma, S6639) and then transferred gradually to PBT ...
-
bioRxiv - Genomics 2020Quote: ... The complete reaction mix was again removed and a slide wash with one 10 minute wash of the wells with 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC was performed ...
-
bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
-
bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were thermally aged overnight at 37 °C and then 60 min at 60 °C, followed by treatments with RNase A (200 µg/mL in 2× SSC, 60–90 min, 37 °C) (Sigma-Aldrich) and then pepsin (50 µg/mL in 10 mM HCl ...
-
bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
-
bioRxiv - Genomics 2022Quote: ... before slides were detached from their hybridization chambers and washed for 10min at 50°C in 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were subsequently washed twice with PBS and once with prehybridization buffer (10% deionized formamide [Millipore] in 2x concentrated SSC [Gibco]) for 5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were then pre-hybridized for 20 minutes at 37°C in wash buffer (2x SSC, 10% formamide (Sigma-Aldrich, F9037)) ...
-
bioRxiv - Immunology 2023Quote: ... the gel was rinsed again with water and soaked in 10X SSC for 30-60 min before capillary transfer to an ImmobilonP membrane (Millipore Sigma), as described previously120 ...
-
bioRxiv - Systems Biology 2023Quote: The coverslip was equilibrated in 1 ml of 30% formamide buffer composed of 2×SSC and 30% deionised formamide (Sigma, F9037) in a 12-well plate for >5 min prior to hybridisation ...
-
bioRxiv - Systems Biology 2023Quote: ... the disulfide bond linking the probe and the fluorophore was cleaved with Cleavage buffer comprising 2×SSC and 50 mM TCEP (Sigma, 646547) for 15 min ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in 20x SSC at 65 °C and stained for 20 min with 5% Giemsa solution (Sigma-Aldrich, GS500).
-
bioRxiv - Evolutionary Biology 2020Quote: ... All primers were obtained from Sigma-Aldrich Ltd ...