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Citations for Millipore Sigma :
651 - 700 of 7897 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... or LIVE/DEAD™ Fixable Blue Cells Stain Kit (#L34957, Thermo; 1:1000 in PBS) and fixation for 15 minutes at RT using 1.85% formaldehyde (F1635, Sigma) in PBS solution before surface staining with antibodies in PBS supplemented with 0.5% BSA [fraction V ...
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bioRxiv - Systems Biology 2019Quote: ... 16] using ChIP grade antibodies against H3K4me3 (Millipore, 07-473) and H3K27me3 (Millipore ...
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bioRxiv - Genetics 2019Quote: ... conjugated on Protein A+G Magnetic beads (Millipore, 16-663). Immunoprecipitated DNA was amplified by PCR and subjected to Illumina sequencing.
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bioRxiv - Biochemistry 2021Quote: ... 16 µL of 11% Tween 20 (Sigma-Aldrich, P-1379) and 200 µL of 7.5% w/v ammonium molybdate (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 ml of pepsin (Sigma-P7000; 16 mg ml-1) was added ...
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bioRxiv - Plant Biology 2021Quote: ... digoxigenin-11-dUTP or biotin-16-dUTP (all Sigma-Aldrich) and FISH were performed according to Jenkins and Hasterok (2007 ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 % Protein G-agarose beads (30 μl) (Millipore, 16-266) were added to lysates after 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and purified by protein A-agarose beads (16-157; Millipore). NIPBL ChIP-seq were from recently published data [46] ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-acetyl-lysine antibody was purchased from MILLIPORE (16-272), anti-actin antibody was purchased from Cell Signaling Technology (4970S) ...
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bioRxiv - Cancer Biology 2020Quote: ... Counterstaining was performed with Mayer-hematoxylin (Sigma-Aldrich MHS-16). Images were taken with a Nikon Eclipse Ci microscope.
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bioRxiv - Genetics 2021Quote: ... ChIP-grade protein A+G magnetic beads (Millipore, 16-663) were added and the enriched chromatin was eluted with 150 uL ChIP Elution Buffer ...
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bioRxiv - Cell Biology 2020Quote: ... for 16 hours and 50 nM Bafilomycin A1 (Sigma B1793) for 2 hours.
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bioRxiv - Neuroscience 2022Quote: ... Neurons were treated at DIV14-16 with 100μM NMDA (Sigma) or L-glutamate (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... TdT enzyme and Biotin-16-dUTP (Sigma #3333566001 and #11093070910) were used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 16 µl of collagenase (100 mg/ml; Sigma-Aldrich #C9697) and 2 µl of dispase (1mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... and Protein A Agarose/Salmon Sperm DNA (Millipore, 16-157) were used for IP of GFP- and YFP-tagged proteins ...
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bioRxiv - Cell Biology 2023Quote: ... 8.00907) and NHS (N-Hydroxysuccinimide, 16 mg/ml, Sigma, 130672) was added to the fluorescent beads solution and incubated for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and methylcyclohexanol (Sigma, 16 µl in 8 ml mineral oil) or 3-octanol (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... enhanced 16 hours later with 10 mM sodium butyrate (Sigma), and harvested 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently with protein A agarose beads (16-125, Millipore) for another 4 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was purified using the GeneElute PCR Clean-Up Kit (Sigma-Aldrich, USA), then labeled with biotin-16-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturers’ instructions with the following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we purified both libraries using the GenElute PCR Clean-Up Kit (Sigma) to remove short library fragments ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturer instructions with the following primers ...
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bioRxiv - Physiology 2022Quote: ... and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020). 300 ng of the eluted DNA was digested overnight at 37°C with the restriction enzymes including USP8 – BstBI and USP48 – SpeI ...
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bioRxiv - Neuroscience 2023Quote: ... and PCR amplification was performed using KAPA2G ReadyMix Kit (Sigma Aldrich, KK5103) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCRs were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) using the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... for 1h at RT, blocked for 1h at RT in blocking solution (0.3% Triton X-100, 0.2% BSA (A4503, Sigma), and 5% goat serum (005-000-121 ...
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bioRxiv - Molecular Biology 2020Quote: ... with Duolink Detection Reagent Green (Sigma, DUO92014) or Orange (DUO92007) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and visualized using chemiluminescence detection (Immobilon, Millipore). Coomassie brilliant blue staining was conducted using Bullet CBB Stain One Super (Nacalai ...
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bioRxiv - Cell Biology 2021Quote: ... Protein detection using anti-HA (H9658, Sigma) or anti-Scp160 antibodies (Gift of R ...
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bioRxiv - Cell Biology 2021Quote: ... Detection was performed using TMB T0565 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Detection was by chemiluminescence with luminol (Sigma) on BioMaxMR film (Kodak) ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Molecular Biology 2020Quote: ... and In situ Detection Reagents Orange (Sigma) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... following detection using PNPP substrate (Sigma, Israel).
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bioRxiv - Immunology 2022Quote: ... Staining with AldeRed ALDH detection assay (Sigma) was done following manufacturer’s recommendation with minor modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... and chemiluminescent detection (ThermoFisher Scientific, Millipore WBKLS0500) were used to develop immunoblots ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was examined with chemiluminescent substrate (Millipore) using the manufacturer’s protocols.
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bioRxiv - Immunology 2022Quote: ... and fixed in RT methanol (Sigma-Aldrich) for 2 minutes prior to PAS staining according to manufacturer’s protocol (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... reverse transcribed with AMV RT (Sigma 10109118001), and then cDNA were quantified in multiplex TaqMan qPCR reactions using commercially available probe sets (Thermo Fisher 4453320 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1U ThermaStop-RT (Sigma-Aldrich) per 50U SSIII to increases RT-PCR specificity ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...