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Citations for Millipore Sigma :
701 - 750 of 2392 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... carefully layered on an RT Ficoll gradient (Sigma HISTOPAQUE®-1119), and centrifuged at 400 x g for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma, St. Louis, MO) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at RT and with DAPI (1/20,000; Sigma) for 5 min at RT ...
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bioRxiv - Immunology 2022Quote: ... for 2 hours at RT and then mounted with Fluoromount (Sigma). Primary Antibodies used were rabbit anti-ACE2 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated using RNAzol® RT (Sigma-Aldrich, R4533) from three biological replicates of WT ...
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bioRxiv - Bioengineering 2022Quote: ... pressure-sensitive foils (Z734438, ThermalSeal RTS™ Sealing Films, Sigma-Aldrich) with holes for air exchange and to enable access to the reservoirs for medium exchange ...
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bioRxiv - Neuroscience 2023Quote: ... all slices were incubated 20 min at RT with DAPI (Sigma). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for 5 min at RT and 200 µL chloroform (Sigma-Aldrich) was added ...
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bioRxiv - Microbiology 2023Quote: ... nuclei were stained with DAPI (1:20,000, 10min, RT; Sigma Aldrich) or SiR-DNA dye (1 µM ...
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bioRxiv - Cancer Biology 2023Quote: Telomerase activity was confirmed using the TRAPeze® RT kit (Sigma) (13–16) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 hr at RT in 0.1 % Calcofluor White (910090, Sigma-Aldrich), and (3 ...
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bioRxiv - Cell Biology 2020Quote: All usual chemicals and primers were from Sigma (St. Louis, MO, USA). Reagents for cell culture ...
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bioRxiv - Genetics 2021Quote: ... 0.2 µM each primer and 0.05 units/µL JumpStart Taq Polymerase (Sigma)) ...
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bioRxiv - Immunology 2022Quote: ... 0.5 μM of both forward and reverse primers (Table 1, Sigma Aldrich), and nuclease free water to a final volume of 50 μL ...
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bioRxiv - Plant Biology 2020Quote: ... primers (10 nmol each) and sterile Milli-Q water (Millipore India, Bangalore) to make up the volume upto 20 μl ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... Primers designed for the site-directed mutagenesis reaction were obtained from Sigma and were HPLC-purified ...
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bioRxiv - Cell Biology 2021Quote: ... Pdk1 (NM_172665) and Pdk2 (NM_133667) (all KiCqStart™ primers KSPQ12012, Sigma Aldrich) were carried out using the SYBRGreen Master mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and desalted or PAGE-purified oligonucleotide primers (Sigma-Aldrich, St Louis, MO) was performed according to manufacturers’ instructions ...
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bioRxiv - Physiology 2020Quote: ... and were purchased from Sigma-Aldrich (see Table 3 for primer details). All reactions were performed in duplicate on 384-well MicroAmp optical plates (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Primers used in this paper (Table S3) were procured from Sigma-Aldrich. PCR products were checked by gel electrophoresis on 1 % (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... was 10 ul consisting of 0.4 of ul forward primer (Sigma Aldrich), 0.4 ul of reverse primer (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... 200nM of gene specific primer- HPLC purified (Sigma-Aldrich, St. Louis, MO) in 25 μL reaction volume ...
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bioRxiv - Developmental Biology 2022Quote: ... on 384-well plates containing 384 primers and Mineral oil (Sigma Aldrich). After sorting ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotide used as primers for amplification (obtained from Sigma-Aldrich or Eurofins) are listed in Supplementary Table S2 ...
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bioRxiv - Biochemistry 2020Quote: ... Powdered chemical reagents and custom DNA primers were purchased from Sigma-Aldrich. Mass spectrometry (MS ...
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bioRxiv - Molecular Biology 2020Quote: ... T7 promoter and T7 terminator primers were obtained from Novagen (Madison, WI). pcDNA3.1 was obtained from Addgene (Cambridge ...
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bioRxiv - Biochemistry 2021Quote: ... All primers used for cloning were designed and ordered from Sigma-Aldrich and are outlined in Table 2 ...
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bioRxiv - Microbiology 2020Quote: ... Oligonucleotide primers were synthesized by Sigma-Aldrich (see Table S1 for sequences). Molecular biology procedures were carried out according to the manufactures’ instructions (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... and primers designed to carry NheI and KpnI restriction sites (Sigma Aldrich). PCR amplified inserts were added at the N-terminus of N protein in pcDNA3.1+ plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... Oligonucleotide primers incorporating desired mutations were synthesised by Sigma Aldrich (Sydney, Australia) and used to generate point mutations in the GlyT2 cDNA/pOTV construct utilising the Q5 site-directed mutagenesis kit (New England Biolabs (Genesearch) ...
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bioRxiv - Plant Biology 2022Quote: ... Primers were designed using Primer3 0.4.0 software and synthesized by Sigma-Aldrich. The presence of pNDN-OGG or pNDH-OCT in F ...
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bioRxiv - Developmental Biology 2022Quote: ... 1ul (10uM [final]) of each of the forward and reverse primers (SIGMA), 2μl of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... All SYBR primers were designed by Sigma Aldrich (see Suppl. Table 1), and Taqman primers were designed by ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 1 µl of 10 mM 5′ HEX labelled oligonucleotide primer (Sigma-Aldrich) and 2 µl ddH2O was incubated at 85°C for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Desalted oligonucleotide primers were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) and can be found in following table ...
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bioRxiv - Molecular Biology 2024Quote: ... using a combination of oligo(dT)23 and random hexamer primers (Sigma). The resulting cDNA was amplified in an AriaMX cycler (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... a SYBR-green qPCR reaction was performed with gene-specific primers (Sigma) as per Nolbrant et al ...
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bioRxiv - Cell Biology 2020Quote: ... For genotyping PCR JumpStart RedTaq PCR master mix (Sigma-Aldrich) was used following the manufacturer’s protocol for cycling with an annealing temperature of 55°C and 35 cycles.
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bioRxiv - Genomics 2020Quote: ... 0.5% Sodium Deoxycholate) with 5.5μl Protease Inhibitor Cocktail Set III EDTA Free (EMD Millipore Corp.539134-1ML) and 11μl Murine RNase Inhibitor (New England BioLabs Inc® M0314L ...
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bioRxiv - Neuroscience 2020Quote: ... a second set of sections were stained using mouse anti-NeuN (Millipore Bioscience Research Reagents; 1:50,000). Following incubation in primary antibody solution as described above ...
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bioRxiv - Plant Biology 2020Quote: ... DNA digestion was performed using the On-Column DNase I Digestion Set (Cat No.: DNASE70-1SET, Sigma) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... volumes of 3 ml of a set of 48 monomolecular odorants (Sigma-Aldrich, St. Louis MO, USA) were pipetted freshly for each experimental day into 15 ml glass vials (27160-U ...
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bioRxiv - Immunology 2022Quote: ... Kmt2d-SET-fl/fl mice were sensitized with 100 μg ovalbumin (OVA #A5503; Sigma, St. Louis, USA) and 1 μg cholera toxin (CT #101B ...
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bioRxiv - Biophysics 2021Quote: ... bisected ovaries were arranged so that the probed surface faced downwards and set in 2% agar (Sigma). Samples were processed through graded alcohol (70% 1h) ...
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bioRxiv - Genomics 2022Quote: These are a subset of a larger set of hydrophobic polytetrafluoroethylene (PTFE, EMD Millipore, Danvers, MA, USA) fluorophore membrane filters (3 μm pore size ...
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bioRxiv - Neuroscience 2022Quote: ... each barcoded set was blocked in CSM containing 10% (v/v) normal donkey serum (Millipore, S30-100ML) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... DAB (Sigma Fast 3,3 Di-aminobenzidine Tablet Set, D-4418 (Sigma-Aldrich Corp, St. Louis, MO, USA)) was used as a counterstain as previously described (Harris et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% Sodium Deoxycholate) with 5.5µl Protease Inhibitor Cocktail Set III EDTA Free (EMD Millipore Corp.539134-1ML) and 11µl Murine RNase Inhibitor (New England BioLabs Inc® M0314L ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase digestion was performed on-column using the On-Column DNase I Digestion Set (Sigma Aldrich, DNASE70) as instructed ...