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Citations for Millipore Sigma :
451 - 500 of 2304 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... All proteins were expressed in Escherichia coli BL21(DE3) Rosetta/pLysS strain (Novagen). Cells were grown in LB medium supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... the bioluminescent Mmr strain was first cultured on Middlebrook 7H10 agar (Sigma-Aldrich) supplemented with 0.5% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... strains were cultured on Murashige and Skoog (MS) medium minus sucrose (Sigma-Aldrich) for 2 weeks at 25°C in the dark.
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bioRxiv - Microbiology 2020Quote: Protoplasts from strains CEA10 and W72310 were generated with Trichoderma harzianum (Millipore Sigma) lysing enzyme and transformed ectopically with linear constructs of gpdA-driven hph hygromycin-resistance gene or H2A:mRFP (CEA10 ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Microbiology 2022Quote: ... Strains were sub-cultured in Mueller Hinton Broth II (MHBII, Sigma, Dorset, UK) or MOPS (Melford ...
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bioRxiv - Microbiology 2022Quote: ... Pseudomonas and Staphylococcus strains were grown on trypticase soy agar (TSA, Sigma-Aldrich) plates with the appropriate antibiotics – for Shigella growth ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with LPS (1ug/ml, Sigma #L4391-1MG, strain 0111:B4) for 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stimulated with 100ng/ml LPS (O111:B4 strain; Sigma catalog # L4391) for 24 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The strains and library were then treated with DMSO only (Sigma-Aldrich, D2650) or 50 µg/mL cycloheximide (EMD Millipore ...
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bioRxiv - Microbiology 2023Quote: All mycobacterial strains were cultured at 37°C in Middlebrook 7H9 broth (Sigma) supplemented with 10% OADC (Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... brucei brucei strain 427 genomic DNA using KOD Hot Start DNA Polymerase (Novagen), and the following forward and reverse primers 5’- AGA GTA TCC AGC TGG GAG TC -3’ and 5’- CTA CCT TGC TCC TAT TGT GC -3’.
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bioRxiv - Biochemistry 2023Quote: ... brucei brucei strain 427 genomic DNA using KOD Hot Start DNA Polymerase (Novagen), and the following primers 5’- CTA CCT TGC TCC TAT TG TGC -3’ and 5’- CTA TAC GTT GCG GTG ATG G -3’.
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bioRxiv - Biochemistry 2023Quote: ... brucei brucei strain 427 genomic DNA using KOD Hot Start DNA Polymerase (Novagen), and the following forward and reverse primers 5’-TTT TTG GAT CCA TGA GTT CGG TAA AGA GTA AAG G-3’and 5’-TTT TTC TCG AGC AAC CGT TTG TCT TCT ATG C-3’ containing BamHI and XhoI restriction sites (underlined regions) ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... coli MFDpir strains were supplemented with 0.28 mM DAP (diaminopimelic acid) (Sigma-Aldrich). When indicated ...
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bioRxiv - Microbiology 2024Quote: ... Each strain was tested for its susceptibility to d-cycloserine (DCS; Sigma-Aldrich), lurasidone (MedChemExpress) ...
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bioRxiv - Microbiology 2024Quote: ... gondii tachyzoites of the indicated strains and lysed in TRI Reagent (Sigma-Aldrich) or Lysis buffer (Jena Bioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting supernatant or ‘secondary virus’ was filtered using 0.45 μm pore size sterile filter (Millipore) and was used immediately to infect large-scale expression cultures ...
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bioRxiv - Cell Biology 2022Quote: ... the medium containing the virus particles was recovered and concentrated with Polyethylene Glycol 8000 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were infected with serial dilutions of virus diluted in 1× minimum essential medium (MEM, Sigma) supplemented with 0.6% BSA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... HeLa cells were incubated overnight in growing media plus virus and polybrene (Sigma-Aldrich, TR-1003). The following day ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG, Sigma # P4338). V6.5 cells were infected in medium containing 5ug/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: Virus propagation was performed in Vero E6 cells in DMEM (Sigma-Aldrich, St Louis, MO, USA) supplemented with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... the virus containing suspension from HEK293T cells was filtered with a 0.45 μM filter (EMD Millipore), supplemented with polybrene (4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Virus was added at the indicated titer in the presence of polybrene (2µg/mL, Sigma, H9268) and the cells were incubated for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were extracted in RIPA buffer (Sigma-Aldrich, R0278) and E-cadherin (MSD ...
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bioRxiv - Neuroscience 2021Quote: ... The crude lysate was treated with 250 U benzonase (Sigma) per 1 ml of lysate at 37 °C for 1.5 h to degrade genomic and unpackaged AAV DNA before centrifugation at 3000 × g for 15 min to pellet cell debris ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell lysates were extracted by radioimmunoprecipitation assay buffer (Millipore) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell lysates were incubated with M2-agarose beads (Sigma; 40 µl M2-agarose /CL6B sepharose 1:1 slurry mix ...
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated with anti-FLAG M2 beads (Sigma Aldrich) or with anti-GFP antibody (Covance ...
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bioRxiv - Cell Biology 2019Quote: ... bacterial lysates were supplemented with 0.1% Tween-20 (Sigma P7949) and incubated with equilibrated glutathione sepharose beads (FisherScientific 45000139 ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were incubated with anti-Flag-agarose beads (A2220, Sigma) or protein A-Sepharose beads (101041 ...
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bioRxiv - Systems Biology 2019Quote: ... Separated protein lysates were transferred to PVDF membranes (Millipore #IPVH00010), which were subsequently rinsed in TBST (Tris-buffered saline ...
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bioRxiv - Biochemistry 2019Quote: ... coli lysate by Ni2+-affinity chromatography using HisSelect resin (Sigma), followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... lysates were incubated with anti-FLAG M2 affinity beads (Sigma) for 2 hr at 4°C ...