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Citations for Millipore Sigma :
351 - 400 of 2304 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The Mpro construct was transformed into Escherichia coli strain Rosetta(DE3) (Novagen) and transformed clones were pre-cultured at 37 °C for 5 h in lysogeny broth supplemented with appropriate antibiotics ...
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bioRxiv - Microbiology 2021Quote: ... All bacterial strains were cultured in LB (Sigma-Aldrich, St. Louis, USA) medium at 37 °C under orbital shaking at 180 rpm.
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bioRxiv - Cell Biology 2021Quote: ... or 1 μg/ml LPS (from Escherichia coli strain 0127:B8, Sigma) (all Peprotech unless noted) ...
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bioRxiv - Cell Biology 2020Quote: ... oxysporum strains towards tomato root exudate and horseradish peroxidase (HRP; Sigma - Aldrich) was measured using a quantitative plate assay as previously described (Turrà et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... For experiments in 24-well plates (using clumpy yMM1174-derived Sigma strains), 3X volume PBS+0.1% Tween-20 (pre-chilled to 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Microbiology 2020Quote: ... coli strain BL21 (DE3) (86) was grown on Mueller Hinton agar (Sigma) in bioassays ...
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bioRxiv - Microbiology 2019Quote: ... Aspergillus strains were cultivated on slopes of Potato Dextrose Agar (PDA) (Sigma) for 7 days at 28°C ...
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bioRxiv - Plant Biology 2021Quote: Single bacterial strains were grown separately in liquid TSB medium (Sigma Aldrich) (15g/ L ...
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bioRxiv - Microbiology 2021Quote: ... all Salmonella strains were grown in Luria-Bertani (LB-Lennox) broth (Sigma) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Doxycycline for a Tet-off strain [16] was purchased from Sigma-Aldrich Chemical Co ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transformed into Escherichia coli BL21 Tuner (DE3) strain (Sigma-Aldrich) or Rossetta DE3 (Novagen ...
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bioRxiv - Microbiology 2023Quote: ... plantarum strain resuspended on sterile phosphate buffered saline (PBS, Sigma-Aldrich, USA) were used as inoculum ...
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bioRxiv - Microbiology 2023Quote: Mycobacterium marinum M bacterial strains were grown on Middlebrook’s 7H11 (Sigma-Aldrich) agar plates supplemented with 10% OADC [oleic acid (Fischer Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... This strain was grown to OD600 ≍0.4 and 0.5 µM isovaleronitrile (Sigma) induced expression of the recombinases ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial strains and pETDuet-1 were purchased from Novagen (New Jersey, USA). All other chemicals were of analytical grade and obtained from domestic companies.
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bioRxiv - Microbiology 2023Quote: ... tuberculosis H37Rv strains were grown in Difco Middlebrook 7H9 medium (Sigma-Aldrich), supplemented with 0.5% glycerol (VWR) ...
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bioRxiv - Cell Biology 2023Quote: EPEC E2348/69 strains were grown in Luria Bertani (LB) (Sigma-Aldrich, St ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Microbiology 2023Quote: ... and complemented strains with Kananmycin and Ampicillin (Sigma Aldrich, 100 µg/mL). The following morning OD600 was measured by plate reader (BioTek Synergy HTX) ...
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bioRxiv - Microbiology 2023Quote: ... all bacterial strains were plated on Muller Hinton (MH) agar (Millipore, USA) and incubated at 37°C for 18 hours ...
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bioRxiv - Microbiology 2024Quote: Brucella strains were grown in tryptic soy broth (TSB, Sigma-Aldrich 22092) at 37°C with 100 rpm agitation supplemented with appropriate antibiotics ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2020Quote: ... then the lysate was treated with RNAseA (Sigma-Aldrich) at 20 ug/ul for 30 min at 37 oC ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were incubated with FLAG agarose (Sigma-Aldrich), GFP-Trap Agarose (Chromotek ...