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Citations for Millipore Sigma :
501 - 550 of 7705 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... all strains were treated without (−NOC) or with (+NOC) 20 μg/ml Nocodazole (Sigma, M1404) as described earlier (Bai et al 2002 ...
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bioRxiv - Evolutionary Biology 2021Quote: Bacterial strains were routinely grown on Luria-Bertani agar (LBA; Sigma-Aldrich, Saint Louis, USA) supplemented with cefotaxime (2 mg/L ...
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bioRxiv - Biophysics 2022Quote: The pET-19b plasmids encoding the CPB constructs were transformed into BL21 (DE3) strain (Sigma) for protein over-expression ...
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bioRxiv - Microbiology 2019Quote: Bacterial strains used in this study (Table S2) were grown in Luria Bertoni (LB) (Sigma), brain heart infusion (BHI ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Metacyclogenesis of epimastigotes (strain Y) was induced by Grace insect medium (Sigma Aldrich, MO, USA) supplemented with 10% FBS and hemin (25 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Aggregatibacter actinomycetemocomitans strain 29522 (ATCC) resuspended in 2% low viscosity carboxy-methyl-cellulose (Sigma-Aldrich). Oral lavage was repeated every week for 5 weeks ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli strains were grown in liquid or solid Luria Bertani (LB) medium (Sigma Aldrich) at 37°C (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α strains were routinely cultivated in lysogeny broth (LB) medium (LB Broth, Sigma Aldrich), 37°C ...
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bioRxiv - Plant Biology 2021Quote: Most bacterial strains were grown in tryptic soy broth (15 g/L, TSB, Sigma-Aldrich) liquid medium or on agar plates containing 15 g/L of Bacto Agar (Difco ...
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bioRxiv - Microbiology 2022Quote: ... All plasmids were maintained as strains and maintained at -80°C in 25% glycerol (Sigma).
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bioRxiv - Microbiology 2021Quote: ... krusei strains were stored at −70 °C in 50% (v/v) anhydrous glycerol (Sigma-Aldrich). They were recovered in yeast extract-peptone-dextrose (YPD ...
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bioRxiv - Biochemistry 2021Quote: RIG-I was expressed using pET28 SUMO vector in Escherichia coli strain Rosetta (DE3) (Novagen). Soluble cell lysate was purified using a Ni2+-nitrilotriacetate (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... all the purified bacterial strains were grown in Luria-Bertani broth (LB, Sigma-Aldrich, Germany) and preserved in glycerol solution to store as −80°C stock.
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bioRxiv - Microbiology 2021Quote: All mycobacteria strains were cultured in Middlebrook 7H9 media (Difco) supplemented with 0.01 % tyloxapol (Sigma), 0.2 % glycerol (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... Strains were assayed in M9 media with 35 g/L monosodium glutamate (MSG; Sigma-Aldrich). The pH was adjusted using hydrochloric acid (Fisher) ...
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bioRxiv - Microbiology 2023Quote: Adhesion of Leptospira strains (Manilae WT, Δmpl36, Δmpl36+, and Patoc) to human PLG (Sigma-Aldrich) was assessed by ELISA as previously described [23] ...
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bioRxiv - Microbiology 2023Quote: ... Overnight cultures of bacterial strains were grown in 5 mL of LB Lennox broth (Sigma) at 37°C shaking at 300 RPM.
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... The other five strains were grown in Brain Heart Infusion (BHI, 53286-500G, Sigma-Aldrich) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... subtilis strains were grown in Spizizen minimal medium (SMM)43 supplemented with 0.4% glucose (Sigma) or 0.4% glucose and 4 mM dimethyl-2-oxoglutarate (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Strains were cultured in chemically defined synthetic media (LSM) containing 200ug/mL streptomycin (Sigma-Aldrich) and 0.5mM L-cystine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... marinum strains were maintained at 30°C in either Middlebrook 7H9 Broth Base (Sigma Aldrich) supplemented with 0.5% glycerol and 0.1% Tween-80 (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA of the bacterial strains was extracted with the GenEluteTM Bacterial Genomic DNA Kit (Sigma). The libraries were prepared with the Rapid barcoding kit (SQK-RBK004 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C wildtype and phage resistant strains were diluted 10 times in Ringer’s solution (Sigma). 50 µL of each 10 times diluted overnight culture (5 x 106 cells ...
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bioRxiv - Microbiology 2023Quote: ... harzianum B97 parental strain with 10 mg/ml lysing enzymes (Trichoderma harzianum, Sigma # L-1412) and 150 µg/ml hygromycin B (Roth ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...