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Citations for Millipore Sigma :
251 - 300 of 5562 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 3.84 g/L yeast synthetic drop-out medium supplements without histidine (Millipore-Sigma), 152 mg/L histidine ...
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bioRxiv - Cell Biology 2023Quote: ... 1.92 g/L yeast synthetic drop-out medium supplements without histidine (Sigma-Aldrich), 2% w/v glucose and 2% w/v agar ...
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bioRxiv - Genetics 2023Quote: URA dropout: 6.8 g/L Yeast Nitrogen Base (YNB; Sigma-Aldrich Cat#Y0626), 20 g/L Glucose (Sigma-Aldrich Cat#G8270) ...
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bioRxiv - Bioengineering 2023Quote: ... coli expression and driven by a T7 promoter and lac operator in a pET28a expression vector (Novagen). Specifically ...
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bioRxiv - Cell Biology 2020Quote: ... The single cells were isolated using cloning rings (Sigma; C1059) and expanded within the ring ...
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bioRxiv - Molecular Biology 2020Quote: Synthetic oligonucleotides used for cloning were purchased from Sigma Aldrich or Integrated DNA Technology ...
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bioRxiv - Immunology 2021Quote: ... individual colonies of cells were isolated using cloning cylinders (Sigma) and expanded in medium containing puromycin ...
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bioRxiv - Microbiology 2021Quote: ... Single colonies were isolated using glass cloning cylinders (Sigma-Aldrich), transferred to 6-well plates (Greiner Bio-One) ...
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bioRxiv - Microbiology 2021Quote: ... Single colonies were isolated using glass cloning cylinders (Sigma-Aldrich), transferred to 6-well plates (Greiner Bio-One) ...
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bioRxiv - Cell Biology 2020Quote: ... Single clones were isolated using Scienceware cloning discs (Sigma Aldrich). Clones were first screened by immunoblotting for loss of Gravin protein expression using two independent antibodies ...
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bioRxiv - Biochemistry 2022Quote: All oligonucleotides used for cloning were obtained from Sigma-Aldrich and are provided in Table S2.
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were cloned into the pT7Blue cloning vector (Novagen) using the megaprimers (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the amplicon cloned into a pT7Blue cloning vector (Novagen). Restriction sites (EcoRI/NdeI ...
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bioRxiv - Plant Biology 2023Quote: ... USA)] by cloning their coding sequences in pET28a(+) vector (Novagen, Netherlands) and pGEX4T1 vector (Amersham Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Stably expressing fluorescent clones were picked using cloning cylinders (Sigma) following fluorescence confirmation ...
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bioRxiv - Cell Biology 2023Quote: Synthetic oligonucleotides used for cloning were purchased from Sigma-Aldrich or Integrated DNA Technology ...
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bioRxiv - Neuroscience 2021Quote: ... transformed with the expression vector pET3a (Novagen) encoding wild-type ...
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bioRxiv - Microbiology 2019Quote: ... The expression vector pET45b (Novagen, Madison, WI) was also digested with the same enzymes ...
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bioRxiv - Plant Biology 2019Quote: ... harboring the pET-28 expression vector (Novagen) containing the coding sequence of the mature form of both enzymes (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... transformed with the expression vector pET3a (Novagen) encoding wild-type ...
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bioRxiv - Biochemistry 2021Quote: ... coli Rosetta (DE3) expression host cells (Novagen). The conditions for expression and purification of the mutant were as described for the wild type protein (24) ...
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bioRxiv - Genetics 2022Quote: ... coli BL21 (DE3) expression host strain (Novagen) was transformed with the pDEST14-PSO2 expression vector ...
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bioRxiv - Biochemistry 2019Quote: The bacterial expression vector pET11d (Merck-Millipore) carrying the human WT RAD51 (WT-pET11d ...
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bioRxiv - Molecular Biology 2023Quote: ... coli expression vector pET-28c (+) (Novagen, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was induced with Doxycycline (Sigma) at 75ng/ml for 72 hours.
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bioRxiv - Plant Biology 2023Quote: ... protein expression in E.coli Rosetta cells (Novagen) was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 30ml Nipagin 10% w/v) spread with yeast paste (Saccharomyces cerevisiae – Sigma-Aldrich #YSC2). Eggs were removed from the agar plate with phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2019Quote: Yeasts (107) were fixed for 1 h at room temperature in 3.2% formaldehyde (Sigma) in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... synthetic media with 0.67% (w/v) Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich), 2% Glucose (Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... SCD medium consisted of 0.7% yeast nitrogen base without amino acids (Sigma, Taufkirchen, Germany), amino acids and 2% glucose ...
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bioRxiv - Biochemistry 2022Quote: ... composed of yeast nitrogen broth without amino acids (YNB, 6.8 g/L; Sigma #Y0626) + 2% glucose (20 g/L ...
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bioRxiv - Biochemistry 2020Quote: ... Yeast were grown in YGLC-glu medium (80 mM sodium citrate pH 4.5 (Sigma), 6.7 g/l yeast nitrogen base w/o amino acids (BD Biosciences) ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1.62 g/L yeast synthetic drop-out medium supplement without leucine (Y1376, Sigma-Aldrich), 2% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... 10% yeast and standard antimicrobial agents (0.1% nipagin and 0.5% propionic acid, Sigma-Aldrich),
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bioRxiv - Microbiology 2019Quote: ... 3 g yeast extract (Carl Roth; 2363.2) and 15 g agar (Sigma; A4800-500G). The NaCl concentration was varied between 5 g ...
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bioRxiv - Biochemistry 2021Quote: ... 1.7 g/L Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate (Y1251 Sigma: 2 μg/L Biotin ...
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bioRxiv - Microbiology 2021Quote: ... 2 % w/v yeast suspension was prepared using 100 mg dry Saccharomyces cerevisiae (Sigma) and 5 mL PBS ...
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bioRxiv - Plant Biology 2021Quote: ... 6.7◻g◻l−1 Yeast Nitrogen Base with Amino Acids (Sigma-Aldrich) and 20◻g◻l−1 Micro Agar (Duchefa Biochemie)) ...
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bioRxiv - Biophysics 2020Quote: ... The RNA from Torula Yeast type VI (~80 bases) was purchased from Sigma-Aldrich. The stock solution of RNA was prepared at the same buffer solution as for protein ...
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bioRxiv - Bioengineering 2022Quote: ... 5 g/L yeast extract) supplemented with ampicillin (50 mg/L) (Sigma-Aldrich, Europe), until the OD600 reached 1.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Fission yeast tropomyosin (Cdc8) cDNAs (WT and D142C) were ligated into pET3a vector (Novagen).
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bioRxiv - Biochemistry 2023Quote: ... yeast were cultivated in YGLC-glu medium (80 mM sodium citrate pH 4.5 (Sigma), 6.7 g/l yeast nitrogen base w/o amino acids (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896; Sigma Aldrich) supplemented with 30 mg/ml lysine ...
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bioRxiv - Bioengineering 2023Quote: ... Reagents required for yeast transformation were obtained from Sigma-Aldrich (lithium acetate dihydrate (LiAc) and deoxyribonucleic acid sodium salt from salmon testes (ssDNA) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 30ml Nipagin 10% w/v) covered with yeast paste (Saccharomyces cerevisiae – Sigma-Aldrich #YSC2). The flies carried out overnight egg lays and eggs were collected from the agar plate with phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then transferred to a medium [1% w/v yeast extract (Sigma-Aldrich), 2% w/v peptone (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... albicans was typically cultured in yeast peptone dextrose (YPD) (Sigma-Aldrich, St. Louis, MO) supplemented with 25 µg/mL uridine aerobically at 30°C.
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bioRxiv - Neuroscience 2024Quote: ... and 0.5% yeast extract (ACROS, 451120010) supplemented with 250 mg/mL glucose (Millipore, 1083371000), 250 mg/mL K2HPO4 (Fisher scientific ...
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bioRxiv - Cancer Biology 2021Quote: Gene expression was measured by quantitative RT-PCR and normalized to β-actin8,48 The SYBR Green quantitative RT-PCR kit (Sigma) was used with the following primers ...
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bioRxiv - Cancer Biology 2022Quote: ... All cloning and sequencing primers were supplied by Sigma (Burlington, MA). All plasmids were grown up in Top10 competent bacteria (Invitrogen ...