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Citations for Millipore Sigma :
1 - 50 of 2344 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... six repeats of CASTing selected binding sites synthesized by SIGMA-ALDRICH were introduced upstream of the TKmini promoter ...
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bioRxiv - Neuroscience 2024Quote: ... and then samples of 40 μg were separated on SDS–PAGE gel in Bio-Rad Mini-PROTEAN Vertical Electrophoresis Cell System and blotted to the Immobilin-P membrane (IPVH00010, Millipore) in transfer buffer (25 mM Tris–HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... CASTing procedure: 12µl of ANTI-FLAG® M2 Magnetic Beads (SIGMA-ALDRICH) were washed twice in 40µl of ESB buffer (20mM HEPES [pH 7.9] ...
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bioRxiv - Microbiology 2019Quote: ... The microfluidic chips were generated by casting PDMS (Sylgard 184, Sigma-Aldrich) into 3D-printed molds ...
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bioRxiv - Molecular Biology 2021Quote: ... drop casting of 3 µl graphene oxide suspension (763705-25ML, Sigma Aldrich), diluted in water to 0.2 mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... followed by drop casting of 0.01% poly-L-lysine solution (Sigma Aldrich) in the electrodes area ...
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bioRxiv - Pathology 2024Quote: ... This mixture was then pipetted into Mantarray casting wells containing 50µL EHT culture media with 0.3U human thrombin (Sigma-Aldrich). EHTs were then incubated for one hour at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen). To obtain single mutants ...
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bioRxiv - Biophysics 2020Quote: ... Prior to the first casting, each master mold was vapor deposition silanized with Trichloro (1H, 1H, 2H, 2H-perfluorooctyl) silane (448931, Sigma-Aldrich) in a vacuum desiccator (Scienceware ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Sample preparation was done by drop-casting of C-Mn3O4 NP solution on 300-mesh amorphous carbon-coated copper grids (Sigma, USA) and allowed to dry overnight at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... a vertical scratch was made with a 10μl tip and 50 µg/mL mitomycin C (Sigma Aldrich) was added to the growth medium to inhibit the cell proliferation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... placed in plastic tissue cassettes (Sigma-Aldrich [Merck]; Z672122) and fixed in 3.7% formalin pots (CellPath ...
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Huge and variable diversity of episymbiotic CPR bacteria and DPANN archaea in groundwater ecosystemsbioRxiv - Microbiology 2020Quote: ... with a 30 kDa ultrafiltration cassette (Millipore Pellicon 2 Biomax). Aliquots of 5 μL were taken directly from the suspensions and deposited onto 300 mesh lacey carbon coated Cu-grids (Ted Pella ...
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bioRxiv - Molecular Biology 2020Quote: ... whereas gene expression cassette were amplified from and pET30a (Novagen) by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... and three 5 kDa MWCO cassettes (PES, Millipore, 0.1 m2). Concentrated cell supernatant was buffer exchanged by diafiltration by 10 mM imidazole-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... the retron cassette encoded in a pET- 21 (+) plasmid (Novagen) and the CspRecT and mutLE32K in the plasmid pORTMAGE-Ec133 were overexpressed using 1 mM IPTG ...
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The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in ArabidopsisbioRxiv - Developmental Biology 2020Quote: Seedlings seven days after germination and grown on vertical plates were incubated for 2min in Lugol solution (Sigma 62650), rinsed in water ...
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bioRxiv - Bioengineering 2021Quote: ... Prior to PDMS casting, the prepared SU-8 molds were silanized with trichloro(1H, 1H, 2H, 2H-perfluorooctyl) silane (Sigma-Aldrich China, Shanghai, China) to facilitate the release of PDMS replicas ...
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bioRxiv - Immunology 2021Quote: ... the kanamycin (km) resistance cassette was amplified from plasmid pET26b (Novagen) using primers k1 and k2 ...
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bioRxiv - Neuroscience 2020Quote: ... containing a puromycin resistance cassette were purchased from Sigma (Supplementary Table S7). Constructs were first tested in HEK cells to assess the knockdown levels ...
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bioRxiv - Microbiology 2021Quote: Tissues were fixed in histology cassettes suspended in 10% formalin (Sigma Aldrich) for 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... One cross-section was placed in a histo cassette (Sigma, H0792-1CS) and fixed in formalin for 2-3 days before transfer to 50% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... a TFF cartridge (Pellicon 2 Mini Cassette, Media: Durapore 0.22 μm, Millipore, USA) was used to cut off particles > 0.2 μm for the isolation of normal size bacteria as well as to remove macromolecules ...
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bioRxiv - Microbiology 2022Quote: ... a TFF cartridge (Pellicon 2 Mini Cassette, Media: Biomax 100 kDa, Millipore, USA) with a molecular weight cut-off (MWCO ...
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bioRxiv - Cancer Biology 2023Quote: ... Cassette deletion was selected for with 10 µM ganciclovir (Sigma Aldrich, cat. no. G2536). Following ganciclovir selection ...
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bioRxiv - Bioengineering 2023Quote: ... and frozen by immersion of OCT cassette in isopentane (Cat. # 277258, Sigma Aldrich, UK) over liquid nitrogen or over dry ice ...
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bioRxiv - Bioengineering 2024Quote: ... The mold was sized to fit a standard tissue embedding cassette (Sigma Cat. #Z672122) to avoid shifting during agarose curing ...
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bioRxiv - Cell Biology 2022Quote: ... washed in sterile H2O and sown on vertical plates with agar medium (1 % [w/v] sucrose, 2.2 g/l MS salts [Sigma/Aldrich], 0.8 % agarose, [pH 5.7]) in cultivation room under a photoperiod of 16 h light:8 h darkness and 23 °C and light intensity 110 µmol/m2/s.
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bioRxiv - Microbiology 2021Quote: ... The second flow-through was concentrated with the Pellicon XL50 cassette with 100 kDa cutoff (Millipore). The resultant concentrate (15-20 mL ...
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bioRxiv - Cell Biology 2020Quote: PCR-based homologous integration cassettes were amplified using the ExpandTM High Fidelity PCR system (Millipore Sigma) from the appropriate plasmid with 80 bp flanking homology to replace the 3’ end of mto2 (primers designed with PPPP at www.bahlerlab.info/resources/)(Bähler et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Recombination of the 4D cassette and nls of GFP was achieved by oral administration of tamoxifen (Sigma) dissolved in corn oil (1:10 ...
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bioRxiv - Microbiology 2019Quote: ... pIEX-10:Neo plasmid carries the neomycin resistance cassette and provides resistance to the antibiotic G418 (Sigma) which allows for selection of transfected cells ...
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bioRxiv - Cell Biology 2019Quote: ... eluted from the gel (GelElute gel extraction kit; Sigma) and quantified via qPCR with a KAPA Library Quantification Kit for Illumina (KAPA Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... and gel purified using GenElute Gel Extraction Kit (Sigma) according to manufacturer’s guidelines and eluted in 50 μl of kit elution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... yeast containing the integration cassettes were selected by growth on YPD plates hygromycin (300 µg/mL; Sigma Aldrich). Colonies were screened by PCR to confirm proper integration of the tag at the correct chromosomal location.
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bioRxiv - Microbiology 2020Quote: ... The supernatant was concentrated via tangential flow filtration using a 10 kDa molecular weight cut-off cassette (Millipore). The concentrated supernatant was dialyzed into 30 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane; Pellicon XL 50, Millipore). The concentrate was further concentrated using a centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Cell Biology 2021Quote: Aggregates embedded in collagen gel were isolated from gel by dissolving the gel with collagenase (C2799; Sigma). Gels were washed with PBS and incubated in 20 μg/ml collagenase in Hanks’ Balanced Salt Solution (H8264 ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration standards (Gel Filtration Markers Kit, MWGF1000, Sigma Aldrich) were separated on similar gradients to estimate protein complex size and included ...
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bioRxiv - Microbiology 2019Quote: ... Samples were gel-purified (Montage DNA Gel Extraction Kit, Millipore) then normalized and pooled to achieve an equal concentration of amplicon DNA from each sample ...
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bioRxiv - Neuroscience 2020Quote: Agarose gel (Sigma) was dissolved in distilled H2O (0.6% wt/vol ...
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bioRxiv - Microbiology 2020Quote: ... Selection for the terbinafine marker based macR:OE cassette and all CRISPRa vector carrying transformants was carried out using 1.1 μg/ml terbinafine hydrochloride (Sigma Aldrich) in the solid transformation medium ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was sequentially filtered through a tangential flow filtration cassette with 0.45 µm pore size (PVDF membrane; Pellicon XL 50, Millipore) and concentrated on a 1,000 kDa MWCO tangential flow filtration cassette (regenerated cellulose membrane ...
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bioRxiv - Microbiology 2019Quote: ... Buffer exchange from glycine-HCl buffer to click reaction buffer (100 mM Na-Phosphate Buffer, pH 7) was performed with Slide-A-Lyzer Dialysis cassettes (Sigma).
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bioRxiv - Microbiology 2020Quote: ... The supernatant was concentrated by tangential flow filtration (TFF) using a Pellicon® 2 mini-cassette system (Millipore, MO, USA) with a 30 kDa pore size ...
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bioRxiv - Microbiology 2021Quote: ... Collected flow-throughs were pooled for replicates of the same sponge species and concentrated with Pellicon XL50 tangential filtering cassette with 100 kDa cutoff membrane (Millipore). The resultant concentrate (15-20 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Viral vectors for shRNA expression as well as empty-cassette plasmids used as vector controls were obtained from the Mission TRC library (Sigma) via the McGill Platform for Cellular Perturbation (clone information and sequences in Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Preparative scale reactions were carried out in 3 mL Slide-A-Lyzer cassettes (10 kDa MWCO, Merck Millipore, Darmstadt, Germany) in combination with custom-made FM containers57 ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were generated by custom DNA synthesis (Twist Bioscience, San Francisco, CA, USA) of the expression cassettes and cloning into the pRSFDuet-1 (Novagen) backbone between the PfoI and BshTI restriction sites ...