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Citations for Millipore Sigma :
501 - 550 of 1130 citations for Vancomycin HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 500 mL LDH buffer solution consisting of 50 mL 1M Tris HCl (pH 8.0, Sigma-Aldrich T3038) and 450 mL ddH2O ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped and the surfactant was cleaved by treating samples with 200 mM HCl (Sigma) at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... mice were injected intraperitoneally with 28 mg/kg of the Akaluciferase50 substrate Akalumine-HCl (TokeOni) (Sigma, 808350) formulated at 16.5 mM in water ...
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bioRxiv - Biophysics 2023Quote: ... it was quenched by adding Tris•HCl (Trizma® base, Sigma-Aldrich, and hydrochloric acid 37%, VWR) at 20–50 mM and incubating for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... PBS was removed and cells were lysed in lysis buffer (20 mM Tris-HCl (Sigma-Aldrich, #T4661) pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... 20% glycerol, pH 7.4) into SEC Buffer 2 (50 mM Tris-HCl, 200 mM NaCl, 0.06% digitonin (Millipore), pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Cell Biology 2019Quote: ... The remaining buffer was removed and the cells were resuspended in 0.8 mL of cold TE buffer (Tris-HCl, pH 7.5, EDTA) with protease inhibitors (aprotinin, pepstatin, leupeptin (Sigma)) and 10 μL was boiled directly in 90 μL of 2x sample buffer (lysate) ...
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bioRxiv - Cell Biology 2020Quote: ... and then lysed on ice in lysis buffer (10 mM Tris-HCl pH 8.0; 10 mM NaCl; 0.5% (v/v) NP-40 substitute (Sigma); 1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 mMTris-HCl in 2x SSC) supplemented with 1 μl of 3.7 mg/ml glucose oxidase (Sigma-Aldrich) and 1 μl of 5 mg/ml Catalase (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... and developed in Fast Red TR and naphthol-AS-MX-phosphate in 0.1 M Tris-HCl pH 8.2 (tablet set; Sigma). After three washes with PBT ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated mitochondria were solubilized with using the solubilization buffer (10mM Tris-HCl pH 7.4,100mM NaCl, 1mM EDTA, 1mM PMSF, protease inhibitor cocktail (Sigma) with 0.5% digitonin ...
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bioRxiv - Microbiology 2022Quote: ... lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1 mM EDTA; Millipore) supplemented with 1× protease inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... a click chemistry reaction cocktail was added to the cells (100 mM Tris HCl pH 7.6, 4 mM CuSO4, 2.5 µM azide-fluor488 (Sigma), 100 mM Sodium ascorbate (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... here the pH of SM or IMF were adjusted to pH4.6 using 6 N HCl (Sigma-Aldrich, Ireland) to remove caseins ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in nuclear lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, 0.8% SDS; Sigma-Aldrich) supplemented with 1xPIC (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed for 30 mins on ice in 1 mL lysis buffer (150 mM NaCl, 2.5 mM MgCl2, 20 mM Tris HCl pH 7.5, 1 % Triton X-100, 250 units Benzonase [Sigma]), samples were then clarified through centrifugation (12000xg ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA fragments containing ssDNA regions were eluted by the addition of SS Elution buffer (10 mM Tris-HCl pH 7.5, 5 mM EDTA and 1 M NaCl, 0.9% caffeine (Sigma)) ...
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bioRxiv - Molecular Biology 2020Quote: ... twice with LiCl wash buffer (10 mM Tris-HCl [pH 8], 250 mM LiCl, 0.5% sodium deoxycholate, 1 mM EDTA, 0.5% IGEPAL CA-630 [Sigma]), and twice with 10 mM Tris-HCl [pH 8] ...
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bioRxiv - Biochemistry 2019Quote: ... The pellet was collected by centrifugation at 110,000 xg for 90 min and the pellet was suspended in 10 ml 10 mM Tris-HCl buffer (pH 7.4) containing 0.1 mg/ml pronase (Sigma) for incubation at 37 °C for 18 h ...
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bioRxiv - Molecular Biology 2019Quote: ... The dried [14C]-SM was re-constituted in Tris-HCl buffer (0.12M, pH7.5, Quality Biological Inc Gaithersburg, MD) with 20mM MgCl2 (Sigma), 0.02% Triton X-100 (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... Cells were spun at 1,000 g for 5 minutes at 4C and resuspended in NP-40 lysis buffer (10 mM Tris-HCl [pH 7.5], 0.05% NP40 [Sigma] ...
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bioRxiv - Plant Biology 2019Quote: ... supernatant was diluted 1 in 10 in pyruvate kinase reaction buffer (85 mM Tris-HCl pH 7.4, 1.7 mM MgSO43.5 mM phosphoenolpyruvate, 23U pyruvate kinase (Sigma-Aldrich)) for 30 mins at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... tissue was ground in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were then stained at room temperature in 0.05 M Tris-HCl pH 7.6 buffer containing 0.5 mg/ml 3-39 diaminobenzidine (DAB, Sigma Aldrich) and 0.01% hydrogen peroxide.
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bioRxiv - Synthetic Biology 2019Quote: ... Tris base (40 mM) adjusted to pH 7.5 with HCl and lysozyme 50 mg/mL (Sigma-Aldrich #L6876) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... parasites were washed with 1X PBS and gently resuspended in 2.5% glutaraldehyde in 0.1M sodium cacodylate-HCl (Sigma) buffer pH 7.2 for 1hr at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 0.1% SDS), two times with LiCl wash buffer (10mM Tris-HCl pH 8.0, 1mM EDTA, 250mM LiCl (Sigma), 0.5% NaDeoxycholate ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed by scraping in a total volume of 80 µL of lysis buffer (containing 50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) and processed for immunoblotting as described in this manuscript under the PUM1 validation subchapter ...
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bioRxiv - Neuroscience 2021Quote: ... cells were scraped with 100 µL lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) supplemented with EDTA-FREE cOmplete Mini protease inhibitor cocktail (Sigma) ...
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bioRxiv - Genetics 2020Quote: ... the cell pellet was resuspended in a premixture of 250 μl cold lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630 (Sigma)) and 50 μl protease inhibitors (Sigma P8340) ...
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bioRxiv - Genetics 2020Quote: ... 50,000 IMR90 cells were collected and resuspended in 50 μl cold lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630 (Sigma)) ...
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bioRxiv - Neuroscience 2020Quote: ... Each run included standard curves of L-kynurenine and L-tryptophan (Sigma-Aldrich; diluted in 0.1 M HCl) with concentrations ranging from 10-500 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining buffer was removed and the cells were resuspended in 0.8 mL of cold TE buffer (Tris-HCl, pH 7.5, EDTA) with protease inhibitors (aprotinin, pepstatin, leupeptin (Sigma)) and 10 μL was boiled directly in 90 μL of 2x sample buffer (lysate) ...
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bioRxiv - Biochemistry 2021Quote: ... in 1 mL of reaction buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5 mM EDTA, and 500 nM bovine SOD [Sigma]). CIII2CIV2 was added (65 nM ...
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bioRxiv - Microbiology 2021Quote: ... The reactions were stopped by adding to 5 μl of reaction 5 μl of Stop Solution (Tris HCl pH 7.5 100 mM, EDTA 12.5 mM, NaCl 150 mM, SDS 1%, Proteinase K (Sigma) 2 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Lysis Buffer (150mM NaCl, 50mM Tris-Hcl, pH7.5, 10% Glycerol, 0.5%NP-40, 1mM MgCl2, 1/1000 Benzonase (Sigma), Phosphatase (PPI ...
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bioRxiv - Cell Biology 2020Quote: ... 30μg of protein was dissolved in sample-loading buffer (5% SDS, 0.2% bromophenol blue, 50% glycerol and 250mM Tris HCl pH8.5, all from Sigma), loaded onto 10% SDS-polyacrylamide gels and subsequently transferred onto nitrocellulose membranes (Protran ...
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bioRxiv - Neuroscience 2022Quote: ... the second saline injection was replaced by either a saline or 20mg/kg cocaine-HCl (Coc; Sigma-Aldrich), resulting in 2 different groups of mice ...
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bioRxiv - Developmental Biology 2022Quote: ... were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40: Sigma). Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866 ...
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bioRxiv - Plant Biology 2022Quote: ... Amino acids were extracted with 200 μL of HCl 10 mM spiked with 0.1 mM Novarline (Sigma-Aldrich) as an internal extraction control ...
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bioRxiv - Genomics 2022Quote: ... Fixed cells were permeabilized in Hi-C lysis buffer (10 mM Tris-HCl, pH 8.0; 10 mM NaCl; 0.2% Igepal CA630; 1× protease inhibitor cocktail [Sigma]) on ice ...
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bioRxiv - Immunology 2022Quote: ... The sensor chip surfaces were regenerated by 30 s exposure to 4M solution of guanidine-HCl (Sigma-Aldrich). The evaluation kinetic parameters of the studied interactions were performed by using BIAevaluation version 4.1.1 Software (Biacore).
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bioRxiv - Genomics 2019Quote: ... the formaldehyde-fixed powder was resuspended in nuclei isolation buffer (10 mM Tris-HCl pH 8.0 (SIGMA, America), 10 mM NaCl (BEYOTIME ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclei preparation and staining was performed by adding 0.08% Pepsin and 2N HCl and IFA/0.5% Tween20 (Sigma). Samples were incubated in the dark ad 100ul anti-BrdU-APC was added while samples were incubated on ice ...
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bioRxiv - Systems Biology 2019Quote: ... Bound glycopeptides were eluted using 4 M urea/100 mM Tris-HCl pH 7.4/400mM GalNAc (Sigma-Aldrich) at RT for 30 min with shaking ...
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bioRxiv - Biochemistry 2019Quote: ... Collagen II was a pepsin-digested guanidine-HCl-extract from a bovine joint cartilage purchased from Sigma-Aldrich/Merck ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were harvested and lysed in RIPA buffer (50mM Tris-HCl [pH 7.4], 150mM NaCl, 1% NP40, 0.25% C24H39O4Na, 1mM EDTA, 1mM NaF, and Protease Inhibitor Cocktail [Sigma]). Coimmunoprecipitation experiments were performed as described previously[77].