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Citations for Millipore Sigma :
251 - 300 of 6929 citations for VEGF D Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... plus D-glucose (EMD) and D-galactose (Sigma). Cultures were grown in a humidified incubator (Infors Multitron ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... D-mannitol and D-trehalose dihydrate (Sigma-Aldrich) at a concentration between 50 and 500 mg/mL were prepared by simple mixing (Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Bioengineering 2023Quote: ... This cell inoculated hydrogel precursor was injected into central tissue lane of MFOC devices and incubated for 30 minutes prior to filling the side channels and media reservoirs with endothelial cell growth medium (VEGF kit) containing 25 µg/mL aprotinin (Sigma-Aldrich) to inhibit fibrin degradation.
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... and Hek293 (ATCC CRL-1573, embryonic kidney) were cultured in DMEM high glucose (Sigma-Aldrich), all supplemented with 2mM glutamine (Biochrome) ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293-GNTI cells were transfected with 1 mg DNA and 3 mg PEI (Sigma-Aldrich) per liter of cells at a density of 1.3-1.8E6 cells/ml ...
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bioRxiv - Cell Biology 2022Quote: SLC1A2/A3-expressing HEK293 Flip-In cells were plated on Poly-L-lysine hydrobromide (Sigma)-coated glass coverslips and induced with doxycycline (1 μg/ml ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... HEK293 and HZ1 cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC number CRL-1573) were cultured in Dulbecco’s Modified Eagle’s medium (DEME) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... SH-SY5Y and HEK293 cells were maintained in Dulbecco’s Modified Eagles Medium (DMEM; Sigma Aldrich) with 10% and 5% FBS respectively ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Physiology 2019Quote: Sperm from the cauda epididymides of 2-month-old C57Bl/6N male mice were allowed to swim out in Embryomax Human Tubal Fluid medium (HTF, Millipore, MR-070-D) and incubated for capacitation ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently with 5IU of human chorionic gonadotropin (hCG) (Intervet, GesmbH) were mated and zygotes were isolated in M2 media (Merck Millipore, MR-015P-D) and cultured in KSOM medium (Cosmo Bio Co. ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The cauda epididymis was quickly cut into pieces and incubated in 1 ml pre-warmed human tubal fluid (HTF) (Millipore, MR-070-D) for 15 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Cell Biology 2020Quote: ... 200nM actinomycin D (act D) (Sigma-Aldrich, St. Louis, MO), an equivalent volume of dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2020Quote: ... [12C]D-d7-glucose(2g/L) (97% D, Sigma-Aldrich) in 100% D2O ...
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bioRxiv - Bioengineering 2023Quote: ... D-(–)-2-Amino-5-phosphonopentanoic Acid (D-APV, Sigma-Aldrich) 80 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-Deoxy-D-glucose (2DG, Sigma-Aldrich, #D-8375-1G) 1M ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Cytochalasin D (Sigma; #C8273 ...
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bioRxiv - Neuroscience 2019Quote: Actinomycin D (Sigma) was reconstituted in DMSO (Sigma ...