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Citations for Millipore Sigma :
1 - 50 of 2834 citations for Toxoplasma gondii IgG antigen RH strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... gondii tachyzoites of the indicated strains and lysed in TRI Reagent (Sigma-Aldrich) or Lysis buffer (Jena Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... gondii strains for 24 h then washed with PBS and fixed with 4% w/v formaldehyde (Sigma) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RH ΔKU80Δhxgprt AtTIR1 and RH TatiΔKU80 strains were grown in Human Foreskin Fibroblasts (HFF) (ATCC, SCRC-1041) in DMEM media (Millipore Sigma) supplemented with 10% FBS [56 ...
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bioRxiv - Microbiology 2023Quote: ... gondii antibodies detection was conducted using an anti-monkey IgG FITC conjugate produced in rabbit (Sigma-Aldrich®, USA). T ...
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bioRxiv - Cancer Biology 2022Quote: ... and rh-EGF (Sigma, Cat #E9644). Cells were seeded in previously coated flask with poly-L-ornithine and laminin.
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bioRxiv - Microbiology 2021Quote: ... gondii localized using antibody against the RH strain tachyzoites (67) and ubiquitin was localized using mouse antibody clone FK2 (04-263; EMD Millipore Corporation). Parasites were enumerated per parasitophorous vacuole (PV ...
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bioRxiv - Immunology 2019Quote: ... were loaded with Rh-123 (Sigma-Aldrich) at a final concentration of 1 μg/mL for 30 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Surfactants – Kolliphor RH 40 (Cremophor RH 40, KRH40) was a gift from BASF (Germany) and Span 80 (S80) was purchased from Sigma-Aldrich (Germany). Propylene glycol (PG ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human (rh) Interleukin-2 (IL-2, Sigma-Aldrich), rhTNFα (Peprotech ...
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bioRxiv - Biochemistry 2021Quote: ... The internal Rh standard (Sigma-Aldrich, Merck KGaA, Darmstadt Germany) with a concentration of 10 μg/l was automatically added to each sample by the ICP-MS device ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Genetics 2022Quote: ... Cells were treated with GnRH (0.2uM,[D-Trp6]-LH-RH, Sigma, L9761) for 3hrs and harvested for RNA preparation ...
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bioRxiv - Immunology 2023Quote: ... OVA antigen (Sigma) was dissolved in saline to a 1 mg/mL concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed 1X antigen retriever solution (Sigma, pH 6.0). Tissues were incubated with primary antibody in 5% donkey serum ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml rh-IL21 (Sigma-Aldrich) and/or 1 nM cytarabine (Cytosine β-D-arabinofuranoside ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... gondii were mutagenized with ENU (N-Ethyl-N-nitrosourea, N3385-1G, Sigma Aldrich; 100-200 μg/ml) in DMEM without FBS for 2 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... gondii tachyzoites (ME49/PTG-GFP, MOI 1) or LPS (100 ng/ml, serotype 011:B4, Sigma-Aldrich) for 4 or 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed with pH 6.0 citrate buffer antigen retrieval solution (Sigma #C9999) in a pressure cooker and cooled for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... RH or RHtgd057-HA were stained with rat anti-HA primary antibody (Sigma, clone 3F10) at 1:500 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Genetics 2020Quote: ... coli strain (Novagen) from the pGEX-6P plasmid (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain (Novagen) with a plasmid that encodes lambda phosphatase (Brading et al. ...
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bioRxiv - Biophysics 2021Quote: ... coli strain (Novagen). nsp1 fragments (SARS-CoV N-terminus (1-12) ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain (Millipore). Glycerol stock of transformed bacteria was cultivated in 50 mL Luria Broth (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... bacterial strain (Novagen), M9LB agar media (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen Retriever (Sigma, Cat. No. C9999). The Vectastain Elite ABC Universal kit peroxidase (Horse Anti-Mouse/Rabbit IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Sections underwent antigen retrieval using 1x Citrate buffer pH6.0 antigen retriever solution (C9999, Millipore-Sigma, Burlington, MA), at 60°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... coli strain Rosetta (Novagen) and then purified (Moor et al. ...
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bioRxiv - Immunology 2020Quote: ... Cowan Strain (Sigma-Aldrich), and 6 μg/ml CpG (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Strain BL21(DE3) (Novagen) was used to produce proteins in E ...
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bioRxiv - Cell Biology 2022Quote: ... coli Rosetta strain (Novagen) by isopropyl β–D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... coli strain DH5α (Novagen) was used as a host to construct all the plasmids ...
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bioRxiv - Cancer Biology 2022Quote: ... coli strain Rosetta (Novagen) and purified by Ni-NTA Agarose Resin (Yeasen Biotech) ...
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bioRxiv - Cell Biology 2022Quote: ... coli BL21 strain (Novagen) in LB-Amp medium ...
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bioRxiv - Microbiology 2023Quote: ... coli strain (EMD Millipore) and streaked out on an LB agar plate containing carbenicillin and chloramphenicol ...
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bioRxiv - Biochemistry 2023Quote: ... coli Rosetta strain (Novagen). The cell pellet was resuspended and lysed by sonication on ice in 100 mL of lysis buffer containing 25 mM Tris (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... anti flavivirus group antigen antibody (Millipore, MAB10216). The blots were developed by HRP reaction and imaged with a ProteinSimple FluorChem imaging system.
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bioRxiv - Cell Biology 2019Quote: ... Antigens were retrieved with 0.05 % pronase (Sigma). Sections were blocked with 3 % (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Antibodies against Lewis antigens were from Sigma and Santa Cruz biotechnology ...