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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Thyroid Peroxidase TPO Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Immunolabeling of Flag-tag was done overnight in 4 °C in the above mentioned buffer containing a 1:5,000 dilution of a monoclonal anti-Flag horseradish peroxidase conjugated antibody (Sigma). Membranes were washed 3×10 minutes with PBS ...
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bioRxiv - Biochemistry 2023Quote: The following analysis was done by western blot using Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody (Sigma-Aldrich) or Monoclonal Anti-Myc tag antibody [9E10] (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... The secondary horseradish peroxidase (HRP)-conjugated antibodies directed against rabbit or mouse were used at a 1/20,000 dilution (Sigma). Detection of luminescent signal was performed with a Luminata Western HRP substrate kit (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Cell Biology 2023Quote: ... was followed by 5 % milk block as well as primary antibodies FLAG-M2-Peroxidase (1:2500, Sigma-Aldrich, USA), TULP3 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... biotin (50 µM in H2O, Sigma-Aldrich, B4501), Z-VAD-FMK (50 or 100 µM in DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... 150ml of PBS containing 6mM free biotin (Sigma) was added to the beads ...
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bioRxiv - Neuroscience 2020Quote: ... Biotin (Sigma-Aldrich, MO, US; Cat. No. #B4501) 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 mM biotin (Sigma-Aldrich, St Louis, MI), 5% (v:v ...
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bioRxiv - Biochemistry 2020Quote: ... Dibenzocyclooctyne-PEG4-biotin conjugate (#760749-5MG, Sigma-Aldrich) was used without further purification ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 100 µM D-biotin (Sigma #2031). Human proteins were incubated for 120 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... GAGs were reacted with sulfo-DBCO-biotin (Sigma) (6.7 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM biotin picolyl azide (Sigma-Aldrich 900912), 10 mM sodium ascorbate made fresh (Sigma 11140) ...
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bioRxiv - Cell Biology 2022Quote: ... Biotin labeled bovine albumin (Sigma-Aldrich A8549-10MG).
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bioRxiv - Neuroscience 2022Quote: ... 1 μM D-biotin (Sigma-Aldrich, Cat# B4501), 1% BSA fatty acid-free (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... but additionally incubated with 50 μM biotin (Sigma) for 24 h before harvesting ...
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bioRxiv - Molecular Biology 2021Quote: ... We then added 1 mM biotin (B4501, Sigma) to neutralize the streptavidin molecules not bound to HELB ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mg/ml Biotin-BSA (A8549; Sigma-Aldrich) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... alkylation was performed with biotin-maleimide (Sigma, B1267) in TEA buffer at 1mM final concentration for 2 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mg/ml Biotin-BSA (A8549; Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... or Azide-PEG3-biotin (Sigma-Aldrich, Cat#: 762024) (2 eq. ...
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bioRxiv - Immunology 2021Quote: ... 100 mM D-biotin (Sigma, cat. no. B4639) was added immediately post-transfection to a final concentration of 10 mM ...
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bioRxiv - Microbiology 2022Quote: ... 0.2mM Biotin-16-UTP (Sigma-Aldrich, REF:11388908910) and 0.3mM unlabeled-UTP were added to the reaction mixture to produce biotinylated RNA ...
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bioRxiv - Cell Biology 2023Quote: ... before injection with D-biotin (B4501; Sigma-Aldrich) at the indicated concentration (unless indicated otherwise ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/ml D-biotin (Sigma-Aldrich; B4639), 5 μg/ml insulin ...
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bioRxiv - Systems Biology 2023Quote: ... and labeled with 50 µM biotin (Sigma, B4639) on Day 7 for 24 hours before lysis in RIPA buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2023Quote: ... and Biotin (500 µM, 1 hour; Sigma-Aldrich). Cells were harvested by scraping and washed three times with 5 mL PBS ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 2 nM biotin (B4501, Sigma-Aldrich), to elute bound proteins for subsequent western blot analysis.
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bioRxiv - Microbiology 2021Quote: ... mice were given a single injection of 100µl preformed rabbit peroxidase-anti-peroxidase (PAP) immune complexes (Sigma) intravenously (i.v. ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Genetics 2023Quote: ... The biotin molecules incorporated into the DNA were visualized by indirect immunofluorescent labeling using 1:60 diluted mouse anti-biotin as a primary antibody (Sigma-Aldrich) applied in 1% BSA/1×PBS/0.1% Triton X-100 for 45 min at RT in a humid chamber ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were stained for neurons using NeuN (anti-NeuN antibody, clone A60, biotin conjugated, Millipore Cat. No. MAB377B, 1500 dilution) and visualized using 3,3′-Diaminobenzidine (DAB) ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000). WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta ...
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bioRxiv - Cell Biology 2022Quote: ... The release of the RUSH cargos from the ER-hook was induced by removing the streptavidin supplemented media and addition of biotin supplemented media (3 mM of D-biotin, B4501 Sigma-Aldrich) for the indicated times.
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bioRxiv - Cancer Biology 2022Quote: ... click chemistry reactions were performed on pre-permeabilized samples to conjugate biotin to the EdU-labeled DNA by using Biotin Picolyl azide (Sigma Aldrich). Sonication was performed with a Bioruptor Pico sonicator (Diagenode ...
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bioRxiv - Biophysics 2024Quote: ... Polyethylene glycol (PEG)-8000 (catalog no. P2139-500G) and Bovine Serum Albumin-biotin (BSA-biotin) (catalog no. A8549-10MG) were obtained from Sigma-Aldrich. Round and square # 1.5 coverslips (catalog no ...
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bioRxiv - Biophysics 2024Quote: ... We used the docking strand R4* modified with biotin on its 5’ end and immobilized it onto coverslip surface functionalized with BSA-biotin (A8549, Sigma-Aldrich) and then Neutravidin (31000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection of Gpd1-TAP (p1291) was carried out by polyclonal rabbit peroxidase α-peroxidase (PAP; Sigma- Aldrich; P1291) at a dilution of 1:10,000 ...
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bioRxiv - Physiology 2020Quote: ... rewashed in TBST and covered with a DAB peroxidase chromogen solution (SigmaFAST 3,3’-Diaminobenzidine peroxidase substrate, Sigma-Aldrich) until the complete appearance of the protein bands of interest ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with anti-rabbit IgG antibody conjugated to horseradish peroxidase (Cat # A9044, Sigma Aldrich; 1:10,000 dilution) for 1 hr at RT and proteins visualized using Clarity ECL western blotting substrate (BioRad ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with an affinity-purified specific antibody (1:10,000) and goat peroxidase-conjugated antirabbit IgG (1:20,000, Sigma-Aldrich, USA), for 2 h at 25 ºC ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were further incubated with horseradish peroxidase-conjugated secondary antibody for 1 h and reacted with ECL (Millipore, USA). Finally ...
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bioRxiv - Genomics 2020Quote: ... Membranes were incubated with primary antibody in 5% milk/TBS-T (5% BSA/TBS-T for anti-RUNX1) overnight at 4°C and subsequently incubated with anti-Rabbit IgG peroxidase antibody (1/10,000, Sigma A6667) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Signals were obtained by binding of a secondary anti-rabbit horse radish peroxidase (HRP) linked antibody (1:15000, Sigma-Aldrich) and visualized by exposing the membrane to a charge-coupled device camera (LAS1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... blots were subsequently incubated for 1h at room temperature with horseradish peroxidase-conjugated secondary anti-rabbit/mouse antibodies (1:10000; Sigma). Immunoreactive bands were visualized by chemiluminescence detection of peroxidase activity (SuperSignal West Dura ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by two 10 min washes and a 1 h incubation in peroxidase-labelled anti-mouse antibody (1:2000, Sigma). Membranes were developed by homemade enhanced chemiluminescence and scanned using FluorChem M hardware (Proteinsimple).
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated with secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-human Fab IgG (H+L) (Sigma, A0293) diluted 1:10000 in blocking buffer for 1 h at RT ...