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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... TEV-eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Immunology 2023Quote: ... Metabolites were separated from proteins and exosomes using a 3 kDa MWCO centrifugation unit (UFC910024, Millipore) and centrifuged at 4000 × g for 40 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The extracellular domain (residues 1–270) of BF2*1901 were cloned into a pET21a vector (Novagen) and transformed into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli N-terminal domain (NTD; residues 1-130) of ProQ were cloned into pET15b vector (Novagen), as described (Stein et al ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were detected by western blotting using the following antibodies: Anti-GFP antibody (1:3000 dilution; Millipore), anti-aldolase antibody (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibodies were suspended in antibody buffer containing 1% bovine serum albumin (BSA, Sigma Aldrich, cat. Number A9418) and 0.1% Triton X-100 in D-PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The primary antibodies were diluted in Antibody diluent containing PBS with 0.3% Triton X-100 and 1% bovine serum albumin (Sigma Aldrich) and applied to the sections overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibodies diluted in antibody solution containing 4% NDS (Sigma-Aldrich) and 0.1% Triton-X (Sigma- Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and immunoprecipitates were analyzed on Western blots using mouse monoclonal anti-MYC antibody (1:800; Covance) (for exogenous 14-3-3ε) or anti-FLAG antibody (1:800; Sigma) (for exogenous Cdc25B ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: HBEC6-KT and Calu-3 cells were lysed using RIPA Lysis Buffer containing Protease Inhibitor Cocktail powder (Sigma-Aldrich) for 60-90 minutes at 4°C on a rocker ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam-17785-1-AP) and PSD-95 ((2.5 μg/ml diluted) Abcam-ab12093) using desired concentrations in PBS containing 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... the grid was washed twice for 3 minutes in washing solution containing filtered PBS and 0.2% glycine (Sigma-Aldrich), blocked for 30 minutes in filtered blocking solution containing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were toothpicked onto fresh BG11 agar plates containing 0.015 g/L of L-methionine and different concentrations of 3-deazaneplanocin A hydrochloride (Sigma), sinefungin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were anesthetized prior to experimental procedures in E3 water containing 0.2 mg/ml Tricaine (ethyl 3-aminobenzoate, Sigma). To prevent pigment formation in larvae during imaging experiments ...
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bioRxiv - Immunology 2019Quote: ... Embryos were grown at 28.5°C and kept under anesthesia with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma) during bacterial injections ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ml of alkaline phosphatase (100mM Tris-HCl pH 9.5, 100 mM NaCl, 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in a humid chamber with primary antibodies (Table 3) in 0.1 M PBS containing 10% normal horse/goat serum and PBS-0.3% Triton X-100 (Sigma-Aldrich). After primary antibody incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: For analysing autophagy flux 72-hours old larvae were transferred to fresh medium containing 3 mg/ml chloroquine (Sigma) as described previously (Low et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
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bioRxiv - Cell Biology 2021Quote: ... were immobilized on freshly made 3% agarose pad in a drop of M9 media containing 0.4 mM (0.05%) tetramisole (Sigma Aldrich) and 3.8 mM (0.5% ...
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bioRxiv - Plant Biology 2021Quote: Seedlings were germinated vertically for 3 days on half strength MS plates containing 0.4% Gelzan™ CM (Millipore Sigma), and then transferred to plates containing either 0 (-P ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Immunology 2020Quote: ... the sections were washed 3 times in PBS and permeabilized in PBS containing 0.3% Triton-X100 (#X100, Sigma-Aldrich) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed twice for 10-m in 1x PBS and then incubated for 1-hr in a blocking buffer containing 3-5% Normal Goat Serum (Jackson Immuno Research, 005-000-121) and 0.01-0.03% Triton-X (Sigma, X100), made up in 1x PBS ...
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bioRxiv - Immunology 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Biochemistry 2019Quote: ... They were then washed with TNC buffer containing 0.05 % Tween 20 (TNC-T) and blocked with 3% bovine serum albumin (Sigma) in TNC-T buffer ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal sections were used for staining and washed 3 × 10 min in Triton PBS (PBS containing 0.3% v/v Triton X-100, Sigma). For visualization of STN borders we performed an immune-staining for the marker forkhead box protein P2 (FoxP2 ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were lysed in Qiagen “Phosphoprotein lysis buffer” containing 0.25% (v/v) CHAPS with 1% (v/v) phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
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bioRxiv - Bioengineering 2019Quote: ... AGMS-3 cells were cultured in alpha-MEM (Thermo Fischer Scientific) containing 15% fetal bovine serum (F7524, Sigma-Aldrich), 100 U mL−1 penicillin ...