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Citations for Millipore Sigma :
151 - 189 of 189 citations for Tetracycline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced when the growth reached OD600 = 1.0 with 0.2 μg/mL anhydro-tetracycline in dimethylformamide (Sigma-Aldrich). The cultures were incubated for an additional 3 hours before harvesting (5020 g ...
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bioRxiv - Cancer Biology 2020Quote: ... β-actin-tTSKid//– (MR) mice were maintained on drinking water containing tetracycline hydrochloride (100 mg/L) (Sigma-Aldrich, T7660) and 3% sucrose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The antibiotic-treated lines (+T1, +T2, and +T3) were treated with tetracycline hydrochloride (lot number SLBQ2368V, Sigma-Aldrich, Germany) mixed in the malt food in a final concentration of 0.05% (Schneider et al. ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher) supplemented with 10% tetracycline-tested FBS (Sigma), 100 μg/mL Zeocin (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-BirA* TBCCD1 and Flag-BirA* cell lines were incubated for different times in complete media with (a) 0.5 μg/mL or 1 μg/mL tetracycline (SIGMA) and 50 μM biotin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: CHO-K1 cells expressing human α1ATWT or the polymerogenic α1ATH334D mutant under a tetracycline inducible promoter (Ordonez et al., 2013) were cultured in DMEM cell media (Sigma) supplemented with 10% Tet-free serum (Pan-Biotech) ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated for 24h in complete media (and in serum free media for 48h prior to biotin addition for the ciliated condition) supplemented with 1 µg/ml tetracycline (Sigma) and 50 µM biotin (BioShop) ...
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bioRxiv - Neuroscience 2020Quote: ... expression of BirA* or BirA*-cyclin F (wild-type and CCNF variants) was induced by adding 0.1 µg/mL of tetracycline (Sigma-Aldrich) to cell culture media ...
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bioRxiv - Microbiology 2019Quote: ... strains were diluted to 0.005 and tested in biological triplicate in serial dilutions of tetracycline (Sigma Aldrich, St. Louis, MO), clarithromycin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were maintained in strict tetracycline-free conditions until induction with 0.2 μg/mL doxycycline (Sigma-Aldrich, St. Louis, MO) for shRNA and 0.4 μg/mL doxycycline for overexpression ...
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at 7 × 105 cells/mL and RNAi induction was initiated with the addition of 1 µg/mL tetracycline (Sigma) and 1 × 107 cells were isolated every 24 hours for RNA analysis (outlined below ...
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bioRxiv - Microbiology 2021Quote: ... subtilis strains are listed in Table 1 and were grown in BHIS broth or on LB agar plates supplemented with CAM (2.5 μg mL−1) and/or tetracycline (TET, 5 and 10 μg mL−1, Millipore Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... cells (10 × 15 cm plates) were incubated for 24 hours in complete media supplemented with 1 μg/ml tetracycline (Sigma) prior to incubation with 50 μM biotin (BioShop ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (2 × 15 cm plates) were incubated for 24 hours in complete media supplemented with 1 μg/ml tetracycline (Sigma) prior to harvesting similar to the BioID group.
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bioRxiv - Microbiology 2023Quote: ... the tetracycline resistance gene from pACE2 (Geneva Biotech, Genève, CH) and the two MCSs from pACYCDuet™-1 (Novagen, Merck Millipore). Csx23 was inserted into MCS-1 of pRATDuet (NcoI/SalI ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1-hTERT cells constitutively expressing rtTA (reverse tetracycline Trans Activator) were used as target cells) in the presence of 8 µg/mL polybrene (Millipore) for at least 6 hours (maximum 24 hours) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3- to 6-hour-old eggs were collected and placed on fresh standard medium containing 0.25 mg/mL tetracycline hypochloride (Sigma-Aldrich), for all Drosophila strains ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... assay54 (as described by Cokol-Cakmak et al.68) to evaluate the predicted ArcA-mediated synergy between tetracycline and selected compounds (sertraline hydrochloride, Sigma-Aldrich). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891).
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged V737N β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891). No respiratory repression of MCF10A or MB-MDA-231 cells was observed with 200 ng/mL ...
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bioRxiv - Biophysics 2020Quote: ... The stress was present for 30 minutes before induction of the reporter mRNA construct using 10 ng/mL anhydrous tetracycline (aTc, Sigma-Aldrich). The time of aTc induction marked the t=0 time point in imaging experiments ...
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bioRxiv - Cell Biology 2020Quote: ... surviving colonies were pooled together and resulting cultures were analysed for the expression of target protein following induction with increasing amounts of tetracycline hydrochloride (T3383, Sigma-Aldrich). To generate cells stably expressing nucleus–restricted CDKL5 ...
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bioRxiv - Microbiology 2020Quote: ... coli ω7249 strain by electroporation and transformants were selected by tetracycline resistance on a LB plate supplemented with 50 µg mL-1 of diaminopimelate (DAP, Sigma-Aldrich) (30) ...
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bioRxiv - Microbiology 2020Quote: ... early stationary pre-cultures were diluted 1:3000 and induced with sublethal doses of tetracycline (Sigma, 50 or 250 ng/mL) overnight under shaking conditions.
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bioRxiv - Molecular Biology 2020Quote: ΔsgrS and ΔptsG strains were grown overnight in LB Broth Miller (EMD) at 37 °C and 250 rpm using 25 μg/ml kanamycin and 10 μg/ml tetracycline (Tet) (Sigma-Aldrich) respectively ...
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bioRxiv - Microbiology 2020Quote: ... The inhibition solution contained 500 μg ml-1 rifampicin and 50 μg ml-1 tetracycline in phosphate buffered saline (Sigma-Aldrich Chemie GmbH ...
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bioRxiv - Microbiology 2020Quote: ... coli strains and during specific stages of the gene deletion procedure (see below) where antibiotic selection with tetracycline (250 µg/mL) (Sigma-Aldrich) was desired ...
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bioRxiv - Microbiology 2023Quote: ... the tetracycline resistance gene from pACE2 (Geneva Biotech, Genève, CH) and the two MCSs from pACYCDuet™-1 (Novagen, Merck Millipore). Csx23 was inserted into MCS-1 of pRATDuet (NcoI/SalI ...
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bioRxiv - Cancer Biology 2021Quote: ... Alonso (Madrid)44.All cell lines were cultured in RPMI 1640 medium with stable glutamine (Biochrom, Germany) supplemented with 10% tetracycline-free fetal bovine serum (Sigma-Aldrich, Germany), 100 U/ml penicillin and 100 µg/ml streptomycin (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of AsCas12a nuclease in the stable HEK 293 T-REx cell line was induced by 100 ng/mL tetracycline for 24 h and assayed by Western immunoblotting using FLAG-tag specific antibodies (Sigma-Aldrich, F1804).
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines except for MSC-52 and MSC-72 were maintained in RPMI-1640 media with stable glutamine (Biochrom, Germany) supplemented with 10% tetracycline-free fetal bovine serum (Sigma-Aldrich, Germany), 100 U/ml penicillin and 100 µg/ml streptomycin (Merck ...
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bioRxiv - Evolutionary Biology 2022Quote: ... uninfected lines were generated for crosses from the isofemale line TPH35CI (Town3+) by treatment with 0.03% tetracycline (Sigma, Castle Hill, NSW, Australia) in cornmeal media for one to two generations (as outlined in Hoffmann et al [49]) ...
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bioRxiv - Cancer Biology 2019Quote: ... All cell lines were cultured in RPMI-1640 medium with stable glutamine (Biochrom, Germany) supplemented with 10% tetracycline-free fetal bovine serum (Sigma-Aldrich, Germany), 100 U/ml penicillin and 100 µg/ml streptomycin (Merck ...
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bioRxiv - Cell Biology 2021Quote: Lentiviral vector aliquots were rapidly thawed at 37°C and diluted in tetracycline-free growth medium containing 10 µg/ml Polybrene (Sigma-Aldrich, Cat# 107689). Cells were transduced overnight in 6-well plates using 1 ml of diluted lentiviral vector ...
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bioRxiv - Evolutionary Biology 2021Quote: ... wAlbB Au (generation 5 post-microinjection) adults were fed 2 mg/mL tetracycline hydrochloride (≥95%, Sigma-Aldrich P/L, Castle Hill NSW, Australia) in a 10% sucrose solution for 10 days before blood feeding ...
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bioRxiv - Genetics 2020Quote: ... Permissive conditions (henceforth on-tet) where the tet-off system is repressed were created by the addition of tetracycline-hydrochloride (Sigma, St Louis, Missouri, USA) to this diet to a final concentration of 1 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... BirA*-Flag or ROBO1-BirA*-Flag fusion-expressing cells were incubated for 24 hours in complete medium supplemented with 1 µg/mL tetracycline (Sigma-Aldrich, Canada, Oakville, ON) and 50 µM biotin (BioShop Canada ...
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bioRxiv - Microbiology 2022Quote: ... the transgene expression was induced in the cells from the tetracycline- inducible promoter by addition of doxycycline (200 ng/ml, #D9891, Sigma Aldrich Saint- Quentin Fallavier, France) to the culture medium.