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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for TROP 2 Human 187a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 100 U/ml human IL-2 (Sigma-Aldrich). After initial activation by plate-bound 0.05 µg of anti-CD3 (homemade ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: Human Embryonic Kidney Cells (HEK293) and HEK293 Flp-In T-Rex cells were grown and maintained in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 FlpIn mycBirA*FOPNL and HEK293 FlpIn mycBirA* cells were incubated for 24 h with 50 μM biotin (Sigma-Aldrich) in the presence or absence of 0.1 μg/ml tetracycline.
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bioRxiv - Immunology 2019Quote: ... and HEK293 and HEK293T cells in DMEM (Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and HEK293 cells with polyethylenimine (PEI, Sigma Aldrich). Details are described in the SI Appendix ...
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bioRxiv - Neuroscience 2022Quote: HEK293 culture (ECACC 85120602, Sigma-Aldrich, Munich, Germany), transfection and electrophysiological recordings were performed as described previously (Grimm et al. ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells were cultured in RPMI medium (Sigma) supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were treated with MG132 (Sigma-Aldrich) at a final concentration of 1 or 10 µM ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 50 units/ mL human IL-2 (Sigma #11147528001).
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bioRxiv - Cell Biology 2020Quote: ... 2 NIH U/mL thrombin from human plasma (Sigma-Aldrich); 2.5 mM CaCl2 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37° C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2020Quote: ... medium supplemented with 2% AB human serum (Sigma-Aldrich, Spain). At that moment ...
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bioRxiv - Cell Biology 2022Quote: ... REVs were incubated with 2 μM human hemopexin (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2020Quote: ... 300 U/mL Roche recombinant human IL-2 (Sigma-Aldrich) and 1 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2019Quote: ... Panc1 and HEK293 in DMEM (Sigma, Cat No. D1145), SH-SY5Y in 1:1 mixture of DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described [46 ...
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bioRxiv - Genomics 2022Quote: ... HEK293 were induced with 3 μg/ml doxycycline (Sigma) and 400 μg/ml of G418 (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were plated on poly-L-lysine (Sigma) coated coverslips in a 12-well plate and transfected with Kv4.1 or Kv4.2 cDNA ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells were cultured in low-glucose DMEM (Sigma) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Millipore) coated glass coverslips ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2022Quote: ... and 2% (v/v) of human serum (Sigma-Aldrich, cat. #H4522). BBB working medium was used for co-culture and assembloids ...
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bioRxiv - Immunology 2020Quote: ... cells were blocked with 2% human AB serum (Sigma-Aldrich, UK) for 10 minutes on ice and then stained with the relevant APC-labelled mAb or hIgG1 isotype (BioInvent ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: Wild type Hek293 cells were grown in DMEM (Sigma D6429) supplemented with 10% FCS ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... while HEK293 and A549 cells were cultured in DMEM (Sigma). Both were supplemented with 10% foetal calf serum (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Hela and HEK293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (PAN Biotech) ...