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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for TROP 2 Human 187a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 1.5 x103/cm2 on 13 mm coverslips in 24 well-plates and cultured in DMEM containing 2% with human AB serum (HS; Sigma Aldrich, H4522) in a 5% humidified CO2 atmosphere at 37°C ...
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bioRxiv - Immunology 2021Quote: HEK293 and transfected derivatives were left unstimulated or stimulated for 24h with 10 ng/mL lipopolysaccharide (LPS) from Salmonella (Sigma), 10 ug/mL isolated S protein ...
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bioRxiv - Neuroscience 2020Quote: ... the HEK293 and primary neuronal cells were plated on the 12mm #1 coverslips coated with poly-D-lysine (Sigma-Aldrich). For the 96-well plate screening assays ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The exogenous expression of ACE2 and TMPRSS2 in the HEK293-C34 cells was induced by addition of doxycycline hydrochloride (1 μg/ml) (Sigma). TMPRSS2-expressing Vero E6 (VeroE6/TMPRSS2 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Vectors were transiently transfected in HEK293-6E cells and antibodies were purified from supernatant using Robocolumn Eshmuno A (EMD Millipore) or Monofinity A Resin prepacked columns (Genscript) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ) were transfected into HEK293 cells using PEI transfection reagent (Sigma). The pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and GIGYF2-KO TLR3-HEK293 cells were either mock-treated or transfected with 1 μg/mL Poly(I:C) (Sigma, P1530) using Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Physiology 2019Quote: Sperm from the cauda epididymides of 2-month-old C57Bl/6N male mice were allowed to swim out in Embryomax Human Tubal Fluid medium (HTF, Millipore, MR-070-D) and incubated for capacitation ...
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bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates from different groups were collected and incubated with magnetic bead RIP buffer containing anti-human argonaute 2 (Ago2) antibody (Millipore, Billerica, MA, USA) and anti-human CPEB2 antibody (Proteintech ...
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bioRxiv - Cancer Biology 2023Quote: Mouse (5 x 105 cells) and human (2 x 105 cells) keratinocytes were seeded onto 12 mm diameter inserts (Millipore, Catalog No. PIHP01250) and cultured in CnT-Prime medium ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...