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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for TREM 1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) Tween 20) containing 5% (w/v) BSA for 1 hour and then incubated with anti-His (Sigma) or anti-GFP antibody (Clontech ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... the transfected HEK 293T cells were treated with MG132 (Sigma-Aldrich) at a concentration of 20 μM for 16 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich, D6171), supplemented with 10% FCS (Eurobio Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... quenched with 10 mL selection media (HEK media as above with 1 µg/mL doxycycline, 100 µg/mL blasticidin (Sigma), and 10nM AP1903 (MedChemExpress)) ...
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bioRxiv - Microbiology 2019Quote: ... were cultured in suspension in serum-free Ex-Cell 293 medium (Sigma) and Expi293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... HEK293E cells were cultured in EX-CELL 293 serum-free medium (Sigma) and incubated with agitation at 37°C and 4.5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293 cells were cultured in MEM medium (Sigma-Aldrich, St. Louis USA) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µg human GST-Rb (48-378 Sigma Aldrich) as substrate and 0.2 mM ATP ...
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bioRxiv - Bioengineering 2019Quote: ... and mouse anti-human nuclei (clone 235-1, Millipore) was performed on sections at differing depths of the tumor ...
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bioRxiv - Bioengineering 2020Quote: Human monocytic THP-1 cells (Sigma Aldrich, lot# 16K052) were cultured and expanded in suspension at a cell density of (0.5–1.5)·106 cells·ml−1 culture medium (RPMI-1640 containing 10 % FBS and 1 % P/S ...
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-human actin (Sigma cat#A2066, 1:2,000), and human anti-S Serum derived from a convalescent individual (1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... or 142 (for human RELN, MAB5366, Millipore 1:400), directed against the N-terminal region ...
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bioRxiv - Neuroscience 2022Quote: ... and Mouse anti-human GAPDH (1:1000, Millipore, MAB374). For visualization Donkey anti-Mouse IRDye® 800CW (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti-human actin (Sigma cat#A2066, 1:2000), mouse ascite anti-SARS S ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-SATB2 (human cells, 1:200; HPA029543, Sigma Aldrich) and Anti-Beta Actin (1ug/ml ...
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bioRxiv - Microbiology 2019Quote: Human Carboxylesterase 1 (CES1) was purchased from Sigma-Aldrich. Capecitabine or deglycocapecitabine (3.25 μl of a 10 mM stock in DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... BRCA1 for human (Millipore Sigma, 07-434, 1:1000), RAD51(Millipore Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... FoxJ1 (rabbit anti-human, Sigma, HPA005714, 1:500 amplified), acetylated α-tubulin (mouse anti-mammal ...
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bioRxiv - Immunology 2020Quote: ... anti-human periaxin (PXN) (Sigma Aldrich HPA001868; 1:300); anti-devil PD-1 clone 3G8 (neat supernatant) ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-human SLC18A1 (1:300 dilution, Sigma HPA063797); AlexaFluor® 488-conjugated donkey anti-rabbit IgG (1:1000 dilution ...
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bioRxiv - Physiology 2022Quote: ... and 1×10-7M Angiotensin II acetate human (Sigma), LOS ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-human nuclei (HuNu 1:50; Millipore, mab1280), rabbit anti-Iba1 (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human pLKO.1-puro-shRNAMAPK11 (Sigma SHCLNG-NM_002751; (TRCN0000199694), and pLKO.1-puro empty vector (Sigma SHC001) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific nucleus(hNA, Millipore, MAB1281, 1:500), anti-BLBP (Cell Signaling,13347s ...
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bioRxiv - Neuroscience 2023Quote: ... 1× SATO [100 µg/ml human apo-transferrin (Sigma), 100 µg/ml BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or mouse anti-human CFTR MAB3482 (Millipore, 1:200) targeting MSD1 ...
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bioRxiv - Bioengineering 2023Quote: ... with 10 µg ml-1 human transferrin (Sigma-Aldrich). Primary human gingival epithelial cells were cultured in keratinocyte serum-free medium (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific Nestin (hNestin, Sigma, Abd69, 1:1000), anti-Sox2 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1% human serum (Sigma Aldrich Cat #H6914), 1% P/S ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-human NEFH (Sigma [ab142]; 1:400), rabbit antihuman SST (ImmunoStar 20067 ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore), and anti-E-cadherin-1 (ECAD ...
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bioRxiv - Immunology 2024Quote: ... anti-human IVL (1:20000, I9018-0.2ML, Sigma-Aldrich), anti-human CLDN1 (1:500 ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...