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Citations for Millipore Sigma :
151 - 200 of 204 citations for TOF Mass Spectrometers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... an exponential growth of tumors with CTL-dependent suppression of the growth and mass-action killing of tumors by CTLs (SiGMA) model (eqn ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Buffer exchange to remove imidazole from the mixture was performed in centrifugal filter with a 10-kDa molecular mass cutoff (Millipore). Following quantification via micro-BCA assay (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Peaks were tentatively identified by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... samples were prepared for ultra-high performance liquid chromatography-high resolution heated electrospray-mass spectrometry (UPLC HR HESI MS) analysis in the dark using ZipTip®C18 (Millipore) purification and resuspension in acetonitrile.
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bioRxiv - Molecular Biology 2021Quote: ... and further concentrated 20-fold using Amicon Ultra Centrifugal Filter Units with a 10-kDa molecular mass cut-off membrane (Millipore Sigma) at 4°C to obtain ALF with a physiological concentration reported within the human lung (1 mg/mL of phospholipid) ...
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bioRxiv - Biophysics 2022Quote: ... we used dextran from Leuconostoc mesenteroides with a molar mass of 450-650 kg/mol (Dextran500k; Sigma-Aldrich Japan, Tokyo, Japan), PEG with a molar mass of 6 kg/mol (PEG6k ...
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bioRxiv - Microbiology 2021Quote: ... The HS and CS samples were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) and quantified by inclusion of [13C6]aniline-tagged standard HS disaccharides (Sigma-Aldrich), as described (Lawrence et al. ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... mice were weighed and injected with intraperitoneally puromycin dissolved in 100 μl of sterile PBS ((0.04 μmol puromycin/ g body mass (Sigma-Aldrich # P8833)) ...
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bioRxiv - Bioengineering 2019Quote: ... The molecular mass of detected peaks was estimated by comparison to the elution times of PEG analytical standard (ReadyCal Set, #02393, Sigma-Aldrich) and of commercial HA with known molecular mass (2-2.2 MDa ...
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bioRxiv - Systems Biology 2019Quote: ... matching against an in-house database (containing information on 600 polar metabolites from the Mass Spectrometry Metabolite Library Supplied by IROA Technologies, Sigma-Aldrich, characterized under the same analysis conditions) ...
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bioRxiv - Neuroscience 2021Quote: ... Unconjugated p-Cresol was identified by comparing with the accurate mass and the retention time of the reference standard (p-Cresol, Sigma-Aldrich) in our in-house library.
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bioRxiv - Developmental Biology 2021Quote: ... Under a stereo dissecting microscope we cut the ampulla region of the oviduct and collected the cumulus oocyte mass in prewarmed M2 medium (Sigma, France). We removed the cumulus mass from ovulated eggs by incubating them for 5-10 min in M2 medium supplemented with 1 mg/ml hyaluronidase (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Compounds with VIP>1 and adjusted p-value<0.05 were tentatively annotated by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed twice with PBS and extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Flash frozen tumors/tissues were pulverized with liquid nitrogen in a mortar and pestle on dry ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 400 µg protein equivalents of tissue were extracted with mass spectrometry grade 80% methanol (Fisher, A456-1) and 20% water (Fisher, W6500) supplemented with 1 nmol DL-Norvaline (Sigma, N7502). Tissue or Insoluble material from cell extracts were used to calculate protein equivalents by resuspension in 0.2 M NaOH ...
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bioRxiv - Microbiology 2022Quote: ... while spotting them onto a polished stainless steel MALDI target in a 1:3 ratio (0.5 μL sample/1.5 μL matrix) with addition of 0.25 μL ProteoMass Normal Mass Calibration Mix (1:10 dilution, ProteoMass™ MALDI Calibration Kit, Sigma-Aldrich) for acquisition of high resolution spectra ...
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bioRxiv - Molecular Biology 2023Quote: ... potassium salt a heterogeneous and Polyadenylic acid (PolyA, cat. no. P9403) with molar mass ranging between 700-3500 kDa were purchased from Sigma Aldrich. Yeast tRNA (tRNA ...
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bioRxiv - Bioengineering 2022Quote: ... The mix for the tumour mass was then set into 96-well plates (Corning® Costar®, Sigma-Aldrich, Dorset, UK) and incubated in 37°C for 15 min to allow crosslinking ...
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bioRxiv - Cell Biology 2023Quote: ... The samples for mass spectrometry (MS) analysis were incubated with Dynabeads protein G that were precoupled with the M2-Flag-antibody (Sigma, F1804) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... TMT-labeled samples for a given mass spectrometry run were then pooled and acidified with 5% formic acid (Sigma, vol/vol). Samples were concentrated using a SpeedVac and resuspended in buffer A (95% water ...
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bioRxiv - Microbiology 2023Quote: Short chain fatty acid species were identified on the basis of precursor ion accurate mass measurements and retention times compared against SCFA commercial standards (Sigma Aldrich), and quantification was performed against calibration curves ...
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bioRxiv - Cell Biology 2023Quote: ... and all identifications of compounds were carried out using reference of the accurate mass and verified using standards purchased from Sigma Aldrich.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sample underwent buffer exchange through a Amicon Ultra-15 100 KDa molecular mass cut-off centrifugal filter unit (Millipore, USA), followed by cleavage with 3C protease at a 1:1 w/w ratio overnight at 4⁰C ...
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bioRxiv - Bioengineering 2024Quote: ... bovine trachea, average molecular mass 28 kDa26) and Dermatan Sulfate (C3788, DS/CS-B, porcine intestinal mucosa) were purchased from Sigma-Aldrich. Chondroitin Sulfate D (400676 ...
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bioRxiv - Systems Biology 2019Quote: ... The resulting chromatogram and the mass spectra of the samples were analysed by comparing with those of amino acids standards (Sigma Aldrich, Germany). All the mass spectra and the chromatograms were analysed out on Thermo XCalibur 2.2 software (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Gels were then process for mass spectrometry or the proteins were transferred to the polyvinylidene difluoride membrane (Merck Millipore, Cat. No. IPVH00010) by Western Blotting (WB) ...
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bioRxiv - Physiology 2020Quote: We collected blood samples for mass spectrometry into 0.6-ml microcentrifuge tubes that we had previously rinsed with 0.1% sodium heparin (H4784, Sigma-Aldrich, St. Louis, MO), and processed the blood as described below ...
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bioRxiv - Biochemistry 2019Quote: ... Dry mass was measured by filtering 20 ml of culture through pre-dried and weighed grade 5 whatmann filter paper (Sigma-Aldrich WHA1005090), drying at 70°C for 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Zygotes in cumulus mass were released from the ampulla of the oviduct into M2 medium containing hyaluronidase (Millipore, Cat# MR-051-F) at embryonic day 0.5 (E0.5) ...
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bioRxiv - Plant Biology 2023Quote: ... Sugars and were identified by comparison of retention time and mass spectra with authentic standards (Sigma, Sigma-Aldrich, St. Luis, MO, USA), while other metabolites were identified using NIST14 (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... Sugars and were identified by comparison of retention time and mass spectra with authentic standards (Sigma, Sigma-Aldrich, St. Luis, MO, USA), while other metabolites were identified using NIST14 (Agilent ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a partial number of compounds were tentatively identified by comparison of retention indices and mass spectra with those of authentic reference standards (Sigma-Aldrich Corporation), with published databases (Wiley Registry of Mass Spectral data-7th edition and NIST Chemistry WebBook ...
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bioRxiv - Genomics 2023Quote: ... Ammonium formate for mass spectrometry and toluene of analytical standard quality were from Sigma-Aldrich (Sigma Chemical Co., St Louis, MO, USA). Mass spectrometer calibration solutions ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was concentrated in an Amicon Ultra-15 50 KDa molecular mass cut-off centrifugal filter unit (Millipore, Burlington, MA, USA) and further purified by SEC on a Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Identification of the VOCs was based on a combination of computer matching with mass spectral libraries (NIST 14) and retention index values derived from an n- alkane solution (c5 to n-c18) (Sigma Aldrich Co.). Only compounds with match factor scores ≥ 70 and signal-to-noise ratios ≥ 10 were selected for quantitation ...
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bioRxiv - Biochemistry 2021Quote: ... a 1 mM stock solution of each glycan was first prepared by dissolving known mass of the glycan in ultrafiltered Milli-Q water (Millipore, Billerica, MA, USA). Aliquots of these solutions were then mixed ...
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bioRxiv - Biochemistry 2021Quote: ... were combined and concentrated to approximately 150 µL in centrifugal filter units with a 3 kDa molecular mass cutoff (Amicon ultra centrifugal filters, 0.5 ml, Millipore, centrifugation at 14,000 g).
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bioRxiv - Microbiology 2022Quote: ... by transferring a loopful of cell mass to 100 μl of Phosphate buffered saline (PBS) buffer and pre-digested with Metapolyzyme (Millipore Sigma, Cat.: MAC4L) for 4 hours prior to bead beating and column extraction ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and the compounds were identified based on their mass spectra and with the aid of a standard solution (Sigma-Aldrich Chemical Co., USA) with synthetic linear hydrocarbons from C21 to C40 ...
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bioRxiv - Biochemistry 2021Quote: ... Identification of FAME was done by comparison of retention times and mass spectra with those of authentic reference chemicals (FAME: reference mixture of 37 FAME, Sigma-Aldrich, Deisenhofen, Germany). As labelled reference ...
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bioRxiv - Molecular Biology 2022Quote: ... CAS-No: 75-09-2) for gas chromatography-mass spectrometry (GC-MS) was SupraSolv grade purity and obtained from Sigma-Aldrich (Darmstadt, Germany). Sodium chloride (NaCl ...
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bioRxiv - Biochemistry 2020Quote: Samples were analysed by mass spectrometry in negative ESI single ion scans set for mass ions of 852.4 m/z (ESI-) (malonyl-CoA, Sigma-Aldrich, Cat. No. M4263), 1032.9 m/z (ESI- ...
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bioRxiv - Cell Biology 2021Quote: ... After alkylation, 5 µg of mass spectrometry (MS) grade trypsin (Fisher, PI90057) dissolved in 5 µL 50 mM acetic acid (Sigma, 45754-100ML-F) was added to proteins on beads ...
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bioRxiv - Microbiology 2023Quote: ... mixture and rS1/rS1-ADPr (32P) mixture in 1 x degradation buffer were incubated with either 0.2 μg Trypsin (Sigma, EMS0004, mass spectrometry grade) or Millipore water as a negative control at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and concentrated 20-fold using Amicon Ultra Centrifugal Filter Units with a 10-kDa molecular mass cutoff membrane (Millipore Sigma, Burlington, MA, USA) to obtain the ALF physiological concentration within the lung (1 mg/mL of phospholipid) ...
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bioRxiv - Plant Biology 2023Quote: ... ABA treatments were initiated by transferring 8-day-old seedlings in mass to plates containing 50 µM ABA (Sigma cis/trans-abscisic acid) or to plates containing vehicle control (Ethanol) ...
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bioRxiv - Microbiology 2020Quote: All chemicals used for preparation of nanoflow liquid chromatography-tandem mass spectrometry (nLC–MS/MS) samples were sequencing grade and purchased from Sigma-Aldrich (St. Louis, MO), unless otherwise stated ...
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bioRxiv - Microbiology 2022Quote: ... assays22 and nanostructure-initiator mass spectrometry (NIMS)23 assays were performed on the supernatant fraction and the remaining material was filtered using Miracloth (Millipore Sigma, Burlington, MA, USA) and dried at 50 °C to determine the biomass dry weight.
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bioRxiv - Evolutionary Biology 2022Quote: ... we first compared retention times and mass spectra to an authentic standard mixture (C7-C40) (Supelco® 49452-U, Sigma-Aldrich, St. Louis, MO) with CHC samples ...