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Citations for Millipore Sigma :
101 - 150 of 1714 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The plates were developed by adding 25μl/well of the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) solution (Millipore Sigma). The reaction was stopped with 0.2N sulphuric acid (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine (TMB) peroxidase substrate (Sigma, St Louis, MO, USA) added ...
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bioRxiv - Immunology 2021Quote: ... with colorimetric signals developed with 100 μL/well of 3,3ʹ,5,5ʹ-Tetramethylbenzidine (TMB) Liquid Substrate (Cat # T0440, Sigma-Aldrich) and stopped using 50 μL/well of 3M HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was finally washed twice in 150 μL HBS-T and once in 150 μL HBS before adding 60 μL 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Sigma #T0440). After developing signal for 15 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed five times with 100 µl/well DPBST and 50 µl/well 3,3′,5,5′-tetramethylbenzidine (TMB) substrate solution (Sigma; T0440) was added to each plate ...
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bioRxiv - Biochemistry 2021Quote: ... the plates were rinsed as mentioned above and the reaction developed using a 3,3’,5,5’-tetramethyl-benzidine substrate (TMB) (#T0440, Sigma-Aldrich) for 15 minutes at room temperature and light-protected ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes probed with HRP-conjugated secondary were developed with a 3,3’,5,5’-teramethylbenzidine (TMB) substrate (Genscript L0022V or Sigma T0565). Developed membranes were scanned and analyzed with ImageJ (National Institutes of Health) ...
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bioRxiv - Biophysics 2020Quote: ... Wells were washed with PBS and secondary antibodies were detected using a TMB substrate (3,3’,5,5’-tetramethylbenzidine; Sigma #T5569) and absorbance measurements at 630 nm.
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bioRxiv - Microbiology 2022Quote: ... Plates were dried and wells were incubated with 50 µl of Tetramethylbenzidine (TMB) Liquid Substrate for Membranes (Sigma Aldrich) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... or IgG2a-HRP conjugated (Southern Biotechnology Associates) and TMB liquid substrate system for ELISA (Sigma-Aldrich, St. Louis, MO). The plates were read in an ELISA plate reader at 405 nM (IgE ...
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bioRxiv - Developmental Biology 2020Quote: ... TMB reagent (Sigma Aldrich) and H2O2 were diluted in 10mM citrate buffer pH 6.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... developed with TMB (Sigma) as recommended by manufacturer and read at A450.
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by adding 100μl/well of Stop Reagent for TMB Substrate (450 nm, Sigma-Aldrich, Taufkirchen, Germany). Absorbance was measured at 450 nm ...
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bioRxiv - Biophysics 2022Quote: ... at a dilution of 1:2000 in TBS + 1 %w/v protease-free BSA and TMB ready-to-use substrate (T0565, Sigma) was used for ELISA procedure ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS six times and 3,3′,5,5′-Tetramethyl benzidine (TMB) substrate (100 μL/well; Millipore, Billerica, MA) was used for detection followed by 0.5 M H2SO4 after 15 min (50 μl/well ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes probed with HRP-conjugated secondary antibody were developed a 3,3’,5,5’-teramethylbenzidine (TMB) substrate (Genscript L002V or Millipore Sigma T0565). Developed membranes were scanned and then processed with ImageJ.
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Microbiology 2022Quote: ... 3,3′,5,5′-Tetramethylbenzidine (TMB, Sigma) substrate was added at 37 °C for 10 minutes (min) ...
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bioRxiv - Bioengineering 2021Quote: ... addition of TMB (Sigma Aldrich) and a Clariostar plate reader (BMG Labtech) ...
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bioRxiv - Immunology 2021Quote: ... Tetramethylbenzidine (TMB) Tablets – (SIGMA, USA), Leishman stain ...
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bioRxiv - Genetics 2020Quote: ... 3,3’,5,5’-Tetramethylbenzidine (TMB) (Sigma) used as the color reagent and read at 630 nm on a BioTek ELx808 absorbance reader.
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bioRxiv - Microbiology 2021Quote: ... developed with TMB (Sigma Aldrich) as recommended by the manufacturer and measured for absorbance at 450 nm.
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bioRxiv - Microbiology 2021Quote: ... 3,3′,5,5′-Tetramethylbenzidine (TMB, Sigma) substrate was added at 37 °C for 10 minutes (min) ...
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bioRxiv - Immunology 2021Quote: ... Tetramethylbenzidine (TMB) Tablets – (SIGMA, USA), Tween 20 (Germany).
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2019Quote: ... or 3,3’,5,5’-tetramethylbenzidine (TMB) (Sigma). Band intensity was quantified using a GS-800™ calibrated densitometer (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... TMB STOP-reagent (S5814, Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of TMB/E (Millipore) was added to each well and the absorbance at 650 nm was read after 20 min ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of TMB/E (Millipore) was added to each well and the absorbance at 650 nm was read after 20 min (BioTek 800 TS) ...
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bioRxiv - Immunology 2023Quote: ... and tetramethyl benzidine (TMB; Sigma-Aldrich) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TMB (Sigma-Aldrich, USA) was added and incubated at RT for 5-15 min till desired blue color was developed ...
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bioRxiv - Cell Biology 2021Quote: ... then TMB was oxidized to TMB diimine by the hydrogen peroxide with horseradish peroxidase (Sigma-Aldrich #P6782). In particular ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Immunology 2020Quote: Pipette 53 μl of TMB (Sigma-Aldrich) to each well.
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bioRxiv - Cell Biology 2021Quote: ... Detection was performed using TMB T0565 (Sigma).
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bioRxiv - Immunology 2022Quote: ... and the TMB (3,3’,5,5’-tetramethylbenzidine, Sigma) was used as a substrate solution for color development ...
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bioRxiv - Microbiology 2022Quote: ... 5’-tetramethylbenzidine (TMB, Sigma-Aldrich, Massachusetts, USA) substrate at room temperature for 5 min ...
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bioRxiv - Immunology 2023Quote: ... and Tetramethyl benzidine (TMB; Sigma-Aldrich, USA) were used ...
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bioRxiv - Immunology 2024Quote: ... 50 μl 3,3′,5,5′-tetramethylbenzidine (TMB; Sigma) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... which was detected with Ultra TMB (Millipore) as described above.
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bioRxiv - Neuroscience 2019Quote: ... Assays were developed using Super Slow TMB (Sigma) and the plates read on a Bio-Tek Synergy 2 plate reader at 650nm.
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bioRxiv - Biochemistry 2022Quote: ... and developed using 10 mg/ml TMB (Sigma), 3% H2O2 in 100 mM sodium acetate ...
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bioRxiv - Microbiology 2022Quote: ... 100 ul of TMB (3,3’,5,5’-tetramentylbenzidine, Sigma) was added to each well and incubated for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... and 100 µL of TMB (Sigma Aldrich, USA) were added to the wells and incubated for 15 minutes at room temperature ...