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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for SpectraDye Antibody Labeling Kit 490 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Labeling of the full-length 487C-ClpB variants with Atto 655-maleimide (Sigma Aldrich) was carried out by incubating ClpB with the dye at 1:1.5 ratio for 5 h in the dark (25 mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... Prior to initiation of the chase labeling with 10mM Uridine-containing (Sigma, U3750-25G) growth medium (100X excess of uridine compared to initial s4U concentrations) ...
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bioRxiv - Microbiology 2021Quote: Biotin labeling in HFFs was initiated with the addition of 50 μM biotin (Sigma B4501 ...
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bioRxiv - Systems Biology 2023Quote: ... After labeling the nuclei with 4’,6-diamid-ino-2-phenylindole (DAPI; Sigma-Aldrich), the cells were mounted with Mowiol 40-88 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: Cre activity for NSC proteomic labeling was induced via tamoxifen (TAM, Sigma-Aldrich #T5648). TAM dissolved in sterile corn oil was administered IP at a dosage of 140 mg TAM/kg body weight/day over a period of five days ...
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bioRxiv - Biophysics 2023Quote: ... the labeling mixtures were spun threw 0.22 micron benchtop spin filters (Ultrafree-MC, Millipore) and loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Developmental Biology 2024Quote: ... Probes were labelled with either the DIG RNA labeling mix (Roche, Sigma product # 11277073910) or the Fluorescein RNA labeling mix (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... We performed nuclear labeling using 600 µL of 2 µg/mL DAPI (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biophysics 2023Quote: Labeling of refolded full-length MYC:MAX with 13C-MMTS (13C-methyl methanethiosulfonate; Sigma-Aldrich) was performed after elution from the Heparin column (see above) ...
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bioRxiv - Bioengineering 2024Quote: ... For the Cy5-labeling of OH dendrimers: Step 1: Fmoc-GABA-OH (Sigma-Aldrich) was coupled with G6-OH using PyBOP (Merck ...
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bioRxiv - Developmental Biology 2021Quote: ... labeling mitotic cells with anti-phospho-Histone H3 (Ser10) (1:2000; Millipore Cat# 05-806) in Blocking Solution overnight at 12°C ...
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bioRxiv - Neuroscience 2022Quote: ... Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma, 11277073910), SP6 RNA polymerase (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Sense and anti-sense Digoxigenin-11-UTP labeled probes (DIG RNA Labeling Mix, Sigma-Aldrich) were synthesized with SP6 and T7 RNA polymerases ...
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bioRxiv - Biophysics 2020Quote: ... and for ultra-high labeling (Fig. 4e) additionally with anti-β -tubulin (T5293; Sigma-Aldrich), each diluted 1:50 in PBS with 2% (w/v ...
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bioRxiv - Biochemistry 2022Quote: The samples for oxidized cysteine content labeling were treated with 500mM iodoacetamide (I6125 Sigma-Aldrich) for 30 minutes followed by quenching with 20mM DTT and reducing with 5mM TCEP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and DIG-labeled RNA probes were generated using DIG RNA labeling mix (Sigma-Aldrich, 11277073910) and T3 RNA polymerase (Promega ...
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bioRxiv - Immunology 2021Quote: ... Cell proliferation dye labeling buffer consisted of 1x PBS/0.1% (w/v) BSA (Sigma Aldrich). IAP antagonists (SMAC mimetics ...
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bioRxiv - Immunology 2023Quote: ... and DNA labeling was done for 24h using BrdU (10 μM Sigma Chemicals, MO, USA). Dually labeled HeLa cells were seeded on ThinCert® Cell Culture Inserts and cultured in hypoxic conditions (1% O2 ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced with PMY labeling medium containing 91 μM of PMY (Sigma-Aldrich) and 45 μM of emetine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 5 dpf larvae with sparse fluorescent protein labeling of PNs were anaesthetized in 0.001% MS222 (Sigma) for 1 minute ...
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bioRxiv - Biophysics 2022Quote: ... The labeling reaction was started by adding 50nmol Atto550-NHS-Ester (Sigma-Aldrich, dissolved in DMSO). After incubation for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... and reverse transcribed using T3 or T7 RNA polymerase and DIG RNA labeling mix (Sigma-Aldrich). Probe qualification and in situ hybridization steps followed (Kramer ...
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bioRxiv - Cancer Biology 2022Quote: ... Differential alkylation was completed by labeling samples with 100 mM heavy labeled ‘d5’ NEM (Sigma-Aldrich) and protein concentration determined via Qubit (adapted from (72)) ...
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bioRxiv - Developmental Biology 2020Quote: ... Dewaxed paraffin sections were analyzed for TdT digoxygenin nick-end labeling with Apoptag Plus (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Probes were then labelled with DIG or Fluorescein (Roche DIG/Fluorescein RNA Labeling Mix, Sigma Aldrich), tagged with Alexa Fluor 594 or 488 dyes (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and stained with 50 μL of a secondary labeling mix containing ExtrAvidin-R-PE (Sigma-Aldrich), anti-human LC-FITC (Southern Biotech) ...
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bioRxiv - Neuroscience 2019Quote: ... nuclear labeling was done with DAPI (4’, 6-diamidino-2-phenylindole; 1 μg/mL, Sigma-Aldrich) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... EdU incorporation was visualized by using a labeling mix containing Copper Sulfate Pentahydrate (Sigma: 209198, 2mM), Ascorbic Acid (Sigma ...
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bioRxiv - Plant Biology 2023Quote: CFDA: A 100 μl drop of 5(6)-carboxyfluorescein diacetate (CFDA, optimum excitation 490 nm, emission 515 nm) (C8166, Sigma, stock solution 50 mg/ml in acetone) was applied to the cut edge of a single cotyledon at a concentration of 0.1 mg/ml in distilled water ...
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bioRxiv - Biochemistry 2020Quote: ... Incubation with kit-specific secondary antibodies (anti-rabbit MINUS Sigma Cat ...
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bioRxiv - Microbiology 2023Quote: The Mouse Monoclonal Antibody Isotyping Reagents Kit (Sigma-Aldrich®) was used for the evaluation of IgG1 and IgM isotypes ...
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bioRxiv - Molecular Biology 2021Quote: ... The biotin labeling was performed for 24 hours in presence of 50 µM of biotin (Sigma Aldrich) in the complete culture medium ...
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bioRxiv - Neuroscience 2020Quote: ... The phenotype of the CldU-IR cells was examined by immunofluorescence labeling using NeuN (1:1,000; Millipore) revealed with a Cy5-goat anti-mouse (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 30 minutes with freshly prepared EU labeling buffer containing 100 mM Tris base (Sigma), 100 μM Alexa Fluor 488 conjugated-azide (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... labeling was based on a protocol previously described76 by killing/demucousing animals in 7.5% NAC (Sigma A7250) in 10 mL 1X PBS for 7.5 min ...
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bioRxiv - Microbiology 2022Quote: ... Deletion of genes was confirmed by Southern blotting analysis using the DIG DNA Labeling Mix (Sigma-Aldrich). Transformation with shuttle vectors was confirmed by PCR using purified genomic DNA as each template ...
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bioRxiv - Genetics 2022Quote: Intracellular ferrous iron was measured by labeling cells with BioTracker Far-red Labile Fe2+ Dye (Millipore Sigma) and analyzing by flow cytometry.
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bioRxiv - Developmental Biology 2020Quote: ... we synthesized fluorescein-labeled RNA probes with the Fluorescein RNA labeling mix (Sigma-Aldrich; Cat. No. 11685619910) and hybridized together with a DIG-labeled probe of another gene ...
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bioRxiv - Developmental Biology 2020Quote: ... we synthesized DIG-labeled RNA probes with the DIG RNA labeling mix (Sigma-Aldrich; Cat. No. 11277073910) and T7 RNA polymerase (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... 12CH2O formaldehyde was used for labeling of WT proteome and heavy 13CD2O formaldehyde (Sigma, cat. no. 596388) for the VPE0 quadruple mutant proteome ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was assessed by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; S7100, Merck Millipore). Images were obtained with a Leica DM3000 microscope with a mounted Leica DFC420 camera ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... Cell cycle was examined by flow cytometry following 5′-bromo-2′-deoxyuridine (BrdU) labeling (BrdU: Sigma-Aldrich, MB5002 ...
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bioRxiv - Cell Biology 2023Quote: ... Proximity labeling was induced overnight by supplementing the medium with 10 μM Biotin (Sigma-Aldrich, cat. #B4501). The cells were lysed ...
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bioRxiv - Developmental Biology 2023Quote: ... Working labeling solution was a 1:10 dilution of the stock solution in 0.3 M sucrose (Sigma). Embryos were injected with labeling solution in the ventral tailbud by mouth pipetting using a glass capillary and collected individually in a 24 well-plate on ice ...
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bioRxiv - Microbiology 2021Quote: ... antibodies and visualized using Immobilon Western Chemiluminescent HRP substrate kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Antibodies against Glut1 and TRAP staining kits were ordered from Sigma. Antibodies against MMP13 ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... F-actin labeling was done using Alexa Fluor 488-conjugated phalloidin (2 u/ml 66 nM, Sigma-Aldrich) and nucleus staining was done with DAPI (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: For fluorescent labeling of Ag85B the green fluorescent dye CFTM 488A succinimidyl ester (Sigma-Aldrich, St. Louis, USA) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... Chemical labeling of live cells was achieved by adding 10 μl of hydroxylamine solution (Sigma: 467804, ~15M solution) and incubated for 10 minutes with gently vortexing intermittently ...