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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The tissues were cut into small pieces with a surgical scissor and digested using the following enzymes: Liberase 4U/ml (Sigma Aldrich, 5401127001), Hyaluronidase 60 U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... were subjected to following protocols to examine the effect of MMC and/or small molecule inhibitors of ISR (ISRIB; Sigma-Aldrich, #19785) or PKR (C16 ...
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bioRxiv - Molecular Biology 2024Quote: ... The SL pulldown from the small RNA fraction was transferred to a 3000 NMWL regenerated cellulose Amicon spin filter (Millipore, Billerica, MA). The removed 5′ cap was enriched by increasing the sample volume with 500 μl decapping buffer and passing the sample through the filter at 10,000 x g twice ...
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bioRxiv - Bioengineering 2021Quote: ... were cut into small pieces and were boiled in a 0.02 M aqueous solution of sodium carbonate (Na2CO3) (Sigma-Aldrich, St. Louis, MO) for 30 minutes to degum the fibroin fibers ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were minced into small pieces with a razor blade and digested with Dispase II (1.25 units/ml, D4693, Sigma-Aldrich, St. Louis, MO), 0.4 mg/ml collagenase IV (C5138 ...
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bioRxiv - Immunology 2022Quote: ... lymph mesenteric nodes were cut in small pieces and transferred into the digestion medium (10mL of complete RPMI with Collagenase D (2mg/mL-Sigma-Aldrich, ref 1108882001) and DNase I (0,1 mg/mL)) ...
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bioRxiv - Immunology 2023Quote: ... a small C18 matrix plug was inserted into a pipette tip after punching out a small amount of C18 matrix from a C18 EmporeTM solid phase extraction disk (Sigma-Aldrich #66883-U) with a 16 gauge blunt ended syringe needle ...
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bioRxiv - Immunology 2024Quote: ... skin biopsies were cut into small places and placed onto a collagen-coated (Collagen G, Sigma Aldrich, Cat: L7213, 1:40 in PBS) foam matrix ...
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bioRxiv - Cell Biology 2022Quote: ... fresh adipose tissue was cut into small pieces and dissociated by enzymatic digestion with collagenase II and XI (800 U/mg; Sigma, Saint-Quentin-Fallavier, France) under shaking in a dry bath for 45 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from cell cultures or from 100 mg of tissue (or less in the case of some small organs and tissues for the screening) using TRI reagent (Sigma– Aldrich, catalog no. T9424) and its concentration was determined using the NanoDrop ND-1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2021Quote: ... the cornea was cut into small pieces with a scalpel and then digested with 2mg/ml collagenase (Sigma-Aldrich, St. Louis, MO, USA, # C0130) diluted in DMEM/F-12 + GlutaMAX™ medium (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Collected animals started a three-day regime of antibiotics within 24 hours of collection (Fields et al., 2018) and were fed dextran beads (Sephadex ‘Small’ by Sigma Aldrich: 50 μm diameter) at a concentration of 0.5 g/100 mL to evacuate their gut content and reduce non-target DNA sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we added an equal volume of stop solution (8 M urea, 1 M Tris–HCl [pH 8.0], and a small amount of Blue Dextran [Sigma Chemical, St. Louis, MO, USA]) to stop the reactions ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: The lentiviral constructs containing PLCβ4 small hairpin RNA (shRNA) or a non-specific shRNA control (Con-sh) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Lentiviral particles were generated by transfecting 293T cells with the expression constructs ...
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bioRxiv - Microbiology 2021Quote: ... The distal parts of the small intestine were collected from each sacrificed mouse and immediately fixed in 10% neutral buffered formaldehyde (Sigma-Aldrich, St. Louis, MO, USA). Also ...
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bioRxiv - Systems Biology 2020Quote: ... Crypts were then cultured in a Matrigel culture system (described below) in small intestinal crypt medium (50X B27 supplement, Life Technologies, 1 mM N-acetyl-L-cysteine, Sigma-Aldrich, in basal culture medium) supplemented with differentiation factors at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... the skin from the tails of adult mice was cut into small pieces and then suspended on Dispase II (Sigma-Aldrich cat. D4693, 2 mg/ml) overnight at 4℃ ...
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bioRxiv - Bioengineering 2022Quote: ... was started by temporal modulation of Wnt signaling using small molecules (CHIR99021, Tocris, R&D Systems, Cat. No. 4423, and IWR-1, Sigma-Aldrich, Cat. No. I0161). The E8 medium was switched out for B27(-)/CHIR ...
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bioRxiv - Neuroscience 2023Quote: ... cells were single cell dissociated using Accutase (Millipore) and resuspended in mTeSR1 with 10 μM ROCK Inhibitor Y-27632 with lentiviruses encoding rrTA and pTetO-Ngn2-puro (generated by the Northwestern University Skin Biology & Diseases Resource-Based Center ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged as single cells using Accutase (Sigma) with Revitacell cell supplement (1/100 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were harvested using Accutase cell detachment solution (Millipore) and washed twice with PBS ...
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bioRxiv - Biophysics 2022Quote: ... BLIM cells are derived from BL26 Blue cells (Novagen) with both the lac operon and F’ episome ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested and lysed using cell lytic (Sigma) containing the protease inhibitors cOmplete EDTA-free (Roche) ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... Cells were passed through a 40μM cell strainer (Sigma) to remove debris and clumps ...
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bioRxiv - Immunology 2019Quote: ... Red blood cells were lysed by resuspending spleen cells in Red Blood Cell Lysing Buffer (Sigma) and incubating on ice for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and MC3T3 cells (3 × 104 cells/well) were seeded into Millicell Hanging Cell Culture Inserts (Millipore) in the six-well co-culture plates ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... infected cells were collected and cell lysates were extracted using CelLytic M cell lysis (Sigma-Aldrich) and extracted cell lysates were loaded to 4-12% Bis-Tris polyacrylamide gel (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Nav1.5-shRNA cells and cells non-targeting shControl cells were maintained in G418 (4 μl/ml, Sigma), blasticidin (2 μl/ml ...
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bioRxiv - Cell Biology 2021Quote: ... For cell length and cell width measurements cells were stained with 0.1 mg/ml calcofluor (Sigma, F3543) and examined under the microscope ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed with cell lysis buffer (Cell signalling) or were treated with Benzonase Nuclease (70664 Millipore) and boiled for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were collected using ice-cold PBS (HEK-293 and A549 cells) or Accutase (Sigma, MDCK cells) to retain surface glycans ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Cells were labeled with cell membrane labeling dye PKH26 (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Stem cells were dissociated as single cells using Accutase (Sigma), re-suspended in mTeSR1 with 10µM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were treated with red cell lysis buffer (Sigma) and washed in FACS buffer ...
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bioRxiv - Molecular Biology 2022Quote: HeLa cells (human cervical cancer cells, ACS7005 Sigma-Aldrich, USA) were cultured in DMEM (Dulbecco’s Modified Eagle Medium ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell viability was analysed with cell counting kit-8 (Sigma) after treating the cells with 10μM GSK716 or 0.1% v/v DMSO (vehicle ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA transfected cells were incubated with cell dissociation buffer (Sigma) and 105 cells/well were seeded in Osteo Assay Surface Plates ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were counted using Muse® Cell Analyzer (Merk Millipore) and plated onto 24-well plates containing the FN-coated coverslips and 500 µl of complete fresh RPMI media (supplemented with 20 ng/mL M-CSF ...
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bioRxiv - Cell Biology 2021Quote: U2OS cells (European Collection of Cell Cultures 92022711; Sigma-Aldrich) were maintained in DMEM (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells were treated with Red Blood Cell Lysing Buffer (Sigma) and resuspended at 2.5 × 107 cells/mL in complete α-MEM medium containing 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2023Quote: ... cells in B cell media were fixed with formaldehyde (Sigma) at final concentration of 1% for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged as single cells using Accutase (Millipore, SF006) and 10 μM of rock inhibitor (Y-27632 ...
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bioRxiv - Microbiology 2023Quote: ... cells were detached with non-enzymatic cell dissociation solution (Sigma). Flow cytometry was performed using a MACSQuant VYB system (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dissociated to single cells with Accutase (Sigma, A6964), plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD ...
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bioRxiv - Neuroscience 2024Quote: ... cells were dissociated into single cell suspension with Accutase (Millipore) and plated on MaGR-coated plates in neural medium with 0.5 μM retinoic acid ...
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bioRxiv - Immunology 2022Quote: ... coli cells (Novagen) and the transformants were isolated on kanamycin containing LB plates ...