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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Siglec 10 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured for at least six days in RPMI 1640 Medium supplemented with 10% dialyzed FCS (Sigma Aldrich), containing either “heavy” lysine (40 g/l ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
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bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in DMEM medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2 mM L-glutamine (Pan-Biotech ...
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bioRxiv - Immunology 2020Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... cell pellets were resuspended with 10% FCS and fixed by adding an equal volume of 4% formaldehyde (Sigma-Aldrich) and mixed for 10 min at room temperature while shaking ...
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bioRxiv - Immunology 2019Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... were cultured in Eagle’s Minimum Essential Medium (ATCC, Middlesex, UK) supplemented with 10% FCS and 0.8 mg/ml geneticin (Sigma-Aldrich). All cells were cultured at 37°C and 5% CO2 in humidified incubators and were free of mycoplasma contamination.
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bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were frozen at 1 x 107 cells per 1 ml of freezing medium (FCS, 10 % DMSO (Sigma-Aldrich)) ...
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bioRxiv - Genetics 2023Quote: ... in supplemented RPMI1640 medium (10% heat-inactivated FCS, 2mM L-glutamine, 50IU/ml penicillin, 50μg/ml streptomycin; Sigma-Aldrich), 1×106 MLN cells/well were stimulated by 50ng/ml PMA and 1μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... taken up in 40ml of FCS-free DMEM supplemented with 480 μl of 10 mg/ml Trypsin (Sigma, T9935) and 800 μl of 50 mg/ml Collagenase II (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with ESC FCS (Millipore Sigma), non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with FCS (Sigma-Aldrich, UK) and penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 5% FCS (Sigma, #F4135), 1% penicillin (10,000 Units/ml ...
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bioRxiv - Immunology 2021Quote: ... FCS was obtained from Sigma-Aldrich. HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 15% FCS (Sigma, C8056), 100 U/ml penicillin-streptomycin (Cellgro ...
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bioRxiv - Molecular Biology 2020Quote: ... in FC-40 (Sigma Aldrich, USA). The devices were incubated at 95 °C overnight and afterward stored at room temperature until further usage for droplet production.
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell pellet was resuspended in 11.7 mL of 10% FCS and then fixed by adding 325 μL of 37% Formaldehyde (Sigma-Aldrich) (final 1% FA ...
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bioRxiv - Developmental Biology 2019Quote: ... in 400ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filetered, C1764 Sigma). Fixation was done in a final concentration of 1% FA (w/o methanol ...
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bioRxiv - Developmental Biology 2019Quote: ... in 250ul of a solution containing 1x PBS complemented with 10% FCS and 5ul Collagenase 10mg/ml (sterile filtered, C1764 Sigma). Fixation was done in a final concentration of 2% PFA for 10 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated FCS and 100 U/ml/100 mg/ml of PS and G418 (SIGMA) at 400 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in HMI-9 medium 53 supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich, St. Louis, USA) and incubated at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (PAN-Biotech) with 10% feral calf serum (FCS) (Sigma-Aldrich), 1% L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Lung & spleen cells were diluted to 1×106cells/ml before incubating with 50 μg/ml SARS-CoV-2 purified spike antigen for 24 h in media (RPMI-1640, 10% FCS, 100U/ml penicillin/streptomycin, 5%NEAA, L-glutamine and HEPES, 0.05 mM β-mercaptoethanol (SIGMA)) ...
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bioRxiv - Molecular Biology 2019Quote: ... BT-474 and MDA-MB-453 cells were cultivated in DMEM medium (Pan-Biotech) supplemented with 10% FCS (Sigma-Aldrich), 2 mM L-Glutamine (Pan-Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... cells were matched-paired and incubated in CO2-free conditions in L-15 medium (supplied with 10% FCS 2mM L-glutamine and 10μg/ml Pen/Strep) and LPS (Sigma-Aldrich) or CSF-1 (Miltenyi Biotec Australia Pte Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... containing 4.5 g l−1 D-glucose and L-glutamine supplemented with 10 % fetal calf serum (FCS; Sigma Aldrich, USA), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (PAN Biotech ...
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bioRxiv - Genetics 2022Quote: ... and 10% heat-inactivated fetal calf serum (FCS) and stimulated with 25 μg/ml lipopolysaccharide (LPS) (Salmonella typhosa origin, Sigma). CD8 T cells were stimulated using anti-CD3 (10μg/ml plate-bound ...
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bioRxiv - Microbiology 2022Quote: ... then cells were replenished with DMEM containing 10% (v/v) FCS and 50 μg/ml gentamicin (Sigma Aldrich, cat# G1397) and incubated for 72 h at 37°C ...
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bioRxiv - Immunology 2023Quote: Cells were cultured in R10 medium, (RPMI 1640 containing 10% heat inactivated FCS, 50μM 2-mercaptoethanol with penicillin and streptomycin, (Sigma-Aldrich) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... 8129) cells were maintained in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma cat. F7524) and 100 U/ml of penicillin/streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown for 3 days in phenol red-free media supplemented with 10% charcoal-stripped FCS (-E2) and subsequently treated with 100nM E2 (Sigma) for the indicated times.