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Citations for Millipore Sigma :
551 - 600 of 3492 citations for Serpin A5 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Human hemoglobin (Hb) (H7379, Sigma) was solubilized in sterile H2O at 10 mg/mL concentration ...
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bioRxiv - Biochemistry 2022Quote: ... 20 µM human hepcidin (Sigma) or purified 11F9 Fab (Pan et al ...
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bioRxiv - Cell Biology 2022Quote: ... or human transferrin (Sigma-Aldrich). BSA was used at a final concentration of 5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Human ab serum (Sigma, Sweden) was purchased and tested negative towards HIV-1 specificity as indicated in the batch reports ...
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bioRxiv - Zoology 2022Quote: ... 5 % of human serum (Sigma) and 50 % of RPMI medium containing DENV2 NGC ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Immunology 2022Quote: Human sera (Sigma Millipore; S7023) was preincubated with rSmCI-1 or rSmCI-1Mut at a concentration of 2μg/ml for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... +10% Human AB serum (Sigma) + 10 ng/ml IL-15 and 10 IU of IL-2 (Stemcell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human Tensin1 (SAB4200283, Sigma), anti-human p-paxillin Y118 (Cell Signaling ...
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bioRxiv - Developmental Biology 2022Quote: ... where human chorionic gonadotropin (Sigma) was used for ovulation according to (Sive et al. ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human-Talin1 (T3287, Sigma), anti-a-tubulin (sc-32293 ...
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bioRxiv - Cell Biology 2023Quote: ... 850 nM human insulin (Sigma), 500 nM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Cell Biology 2023Quote: ... 850 nM human insulin (Sigma), 500 nM IBMX (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... +5% human AB serum (Sigma) +2 mM L-glutamine +100 units penicillin-streptomycin +25 mM HEPES buffer +1x non-essential amino acids (NEAA) ...
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bioRxiv - Cell Biology 2023Quote: ... with 2% human serum (Sigma), then labelled with APC-conjugated anti-mouse CD31 antibody ...
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bioRxiv - Biochemistry 2023Quote: ... Ras (human) (553325, EMD Millipore) was diluted in SEC buffer.
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bioRxiv - Neuroscience 2023Quote: ... human nuclei (1:1000, Millipore), Islet1 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... Human Hemoglobin (Hb) (H7379, Sigma) was solubilized in sterile H2O at 10 mg/mL concentration ...
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bioRxiv - Bioengineering 2023Quote: ... and Thrombin (Human plasma, Sigma). Type I collagen (Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (Sigma-Aldrich, #5051); Rhodamine-conjugated fibronectin (Cytoskeleton Inc. ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... A human IgG (Sigma #I2511) standard curve (ten three-fold serial dilutions starting at 20 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... 2% human serum (Sigma-Aldrich), 1% non-essential amino acids (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... human insulin (I2643-25mg, Sigma) and monothioglycerol (M1753 ...
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bioRxiv - Immunology 2024Quote: ... A human IgG (Sigma #I2511) standard curve (10 three-fold serial dilutions starting at 20 µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with Ccl2 or control Empty constructs ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with shRNA retroviral constructs ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles encoding guide RNA and Cas9 were produced in HEK 293T cells using mouse Irf7 CRISPR lentiviral transfer construct targeting exon 7 (target ID #MM0000202587) of Irf7 (Sigma Aldrich). Lenti CRISPR universal non-target plasmid was used as a control (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and HEK-293T-ACE2-DPP4-TMPRSS2 were maintained by supplementing the culture medium with 1 μg/ml Puromycin (Sigma-Aldrich, USA), and 1.5 μg/ml Blasticidin (InvivoGen ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT host cells harboring the Flp-In™ T-REx system were maintained in culture medium (DMEM-high glucose (Sigma) medium ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...