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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Serine threonine protein kinase greatwall MASTL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
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bioRxiv - Cancer Biology 2022Quote: ... Ras protein level in the pull down was analyzed by by immunoblotting using RAS antibody (Sigma-Aldrich #OP40). Total was lysate was also subjected to immunoblotting to determine RAS expression level as the input.
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bioRxiv - Molecular Biology 2023Quote: ... An equivalent amount of protein lysate was subjected to SDS-PAGE and immunoblotting with antibodies against FLAG (Sigma), V5 (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
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bioRxiv - Biochemistry 2023Quote: ... the proteins bound to the Flag antibodies were eluted using 400 µg/mL of 3xFlag peptide (Sigma, F4799) during 30 min at 4°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Biophysics 2022Quote: ... and an ATP-regeneration system (4 mM ATP, 50 μg/mL creatine kinase [Sigma-Aldrich], 5 mM creatine phosphate [Sigma-Aldrich]). The endpoint fluorescence for complete FITC-casein degradation was determined by addition of porcine elastase (100 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Physiology 2019Quote: ... All solutions were thawed from stocks at the day of experiments and freshly supplemented with creatine kinase (CK, Sigma/Roche, Germany) to ~300 U/ml or ~3 U/well and sodium azide (0.1 M NaN3) ...
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bioRxiv - Developmental Biology 2022Quote: ... Inhibition of EGFR signaling was performed by injecting 80 μg/g of the kinase inhibitor PD153035 (CAS: 183322-45-4; Sigma-Aldrich), dissolved in DMSO/1xPBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Cancer Biology 2023Quote: Fresh EAC tumor samples were transferred into ice cold conditioned PBS (formula: 10uM rho-associated kinase (ROCK) inhibitor Y27632 (Sigma, #Y0503), 2% penicillin-streptomycin sulfate (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The preactivated kinase mixture was then desalted and concentrated using an Amicon™ Ultra-15 30 kDa MWCO spin filter (Millipore) to remove the residual ATP.
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bioRxiv - Biochemistry 2023Quote: ... Serum samples were collected from surviving mice and 3 samples from each group were used for estimation of Creatine Kinase activity Assay Kit (Sigma-Aldrich).
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bioRxiv - Biophysics 2023Quote: ... phosphoenol pyruvate (53 mM PEP, Beantown Chemical, 129745) and pyruvate kinase/lactate dehydrogenase enzymes (2.8% v/v PK/LDH, Sigma, P-0294). For experiments with PRC1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the c-Jun N-terminal kinase inhibitor SP600125 (Cat. S5567) and the c-Myc inhibitor 10058-F4 (Cat. F3680) were from Millipore Sigma.
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bioRxiv - Immunology 2023Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Biochemistry 2023Quote: GS activities were measured using the pyruvate kinase/ lactate dehydrogenase (PK/LDH) coupled enzymes from rabbit muscle ordered from Sigma-Aldrich (containing 600-1,000 units/ml pyruvate kinase 900-1,400 units/ml lactate dehydrogenase) ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... A stock ATPase reaction mix (20X) contained 20 μL of a mixture of pyruvate kinase and lactic dehydrogenase from rabbit muscle (P0294, Sigma Aldrich), 10 μL of 200 mM NADH (CAS# 606688) ...
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bioRxiv - Physiology 2024Quote: ... to activate transient receptor potential kinase ankyrin 1 [TRPA1] channels; 10 µM for 5 min, from a stock concentration of 1 M in DMSO, Sigma-Aldrich); HC-030031 (to block TRPA1 channels ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Genetics 2023Quote: ... The dilution ratios were as follows: 1:1000 for antibodies to kinetochore proteins and 1:100 for antibodies to α-tubulin (Sigma-Aldrich, St. Louis, MO; catalog number T6199). After washing twice for 5 minutes in 1× PBS at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes and immunoblot analyses were carried out using rabbit antibodies directed against ShcA (Millipore), Collagen X (Abcam) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2021Quote: ... and the expression of the bait-protein was detected from the input sample with anti-FLAG antibody (Sigma Aldrich).
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...