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Citations for Millipore Sigma :
101 - 150 of 9314 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 h with a blocking solution containing 3% BSA (BP9703-100; Sigma-Aldrich). Cells were incubated for 1 h with anti-ACBD3 and anti-GM130 primary antibodies followed by 1 h incubation with appropriate Alexa Fluor Dyes ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with blocking solution (1% BSA, 3% Donkey Serum [Sigma: D9663], 0.1% (v/v) Tween in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking and antibody incubation was done using 3% bovine serum albumin (BSA) (Sigma-Aldrich A7906-10G) and 0.3% TritonX (Sigma-Aldrich T8787 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were diluted 1 uL into 3 uL water before monitoring peptide formation electrophoretic TLC (Millipore). TLC plates were equilibrated with pyridine acetate buffer (5 mL pyridine ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-FLAG M2 affinity gel (A2220) and 3 x FLAG peptide (F3290) were from Sigma-Aldrich, protein A or G beads were from Invitrogen (10004D) ...
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bioRxiv - Biochemistry 2022Quote: ... Two sequential elutions were carried out with 100 μl 0.1 mg/ml 3× Flag peptide (Sigma) in RNC buffer at room temperature for 25 min ...
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bioRxiv - Developmental Biology 2023Quote: ... predicted mature peptide regions of ZmRALF1/2/3/5 genes were cloned into plasmid pET32b (Novagen) with gene specific primers as indicated (Supplemental Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... after which the ylmH complexes were eluted using 0.2 mg ml−1 3× Flag peptide (Sigma) in wash buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Neuroscience 2020Quote: HFIP treated Aβ42 peptide (JPT Peptide Technologies) and Aβ42-1 reversed peptide (Sigma) were dissolved in DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich; Cat. No. A7906) in PBST ...
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bioRxiv - Genomics 2022Quote: ... both diluted in 1× TNB (1M Tris HCl pH 7.5, 3 M NaCl-blocking reagent, Sigma-Aldrich) with posterior incubation for 1 h at 37°C ...
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bioRxiv - Immunology 2019Quote: ... followed by blocking of endogenous peroxidase with 3% H2O2 (Sigma H-1009; Sigma-Aldrich Chemie GmbH, Germany) in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking and antibody staining was performed in 3% bovine serum albumin (BSA; Sigma-Aldrich® cat. # A7906) in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with a blocking serum containing 3% bovine serum albumin (Sigma-Aldrich, cat. no. A9647), 10% goat serum (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 2 hours with blocking buffer (3% donkey serum in 0.1 M PBS; Sigma-Aldrich). Primary antibodies (ChAT ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was performed in Millipore blocking reagent (EMD Millipore), followed by incubating the section in Anti-GFP (ab13970 ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking with 1x casein blocking buffer (Sigma) for 4 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... grown for 2.5 hours and arrested by adding 3 μg/ml α-factor (Sigma, custom peptide WHWLQLKPGQPMY) every 60 min for 120 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... were isolated from either naïve or infected or tumour-bearing HHD or BALB/c mice and were cultured in the presence of HLA-A2 restricted IAV M1 peptide 158-66(GILGFVFTL)(Cambridge peptide) or NY-ESO-1 peptide 157-65 (SLLMWITQC)(Sigma peptide) or H-2Kd binding IAV NP peptide 147-55(TYQRTRALV ...
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bioRxiv - Neuroscience 2021Quote: ... blocking and permeabilization was performed with DPBS + 3% BSA + 0.1% Triton X-100 (Millipore Sigma cat. no. X100) for 30 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... cells are washed 3 times with PBS and blocking solution is added (Bovine Serum Albumin, BSA. Sigma; A7906) at 3% in PBS for at least 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and incubation in blocking solution (3% bovine serum albumin, 0.5% Triton x-100, Sigma, St. Louis, MO, USA) in 0.1M PBS ...
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bioRxiv - Bioengineering 2022Quote: ... After blocking for 1 h by adding a drop of 3 % bovine serum albumin (A9418, Sigma-Aldrich, USA) onto exposed tissue ...
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bioRxiv - Cell Biology 2022Quote: ... blocked for 60 min in blocking solution (PHEM with 3% bovine serum albumin(BSA) (Sigma-Aldrich, Cat# A2153)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking and antibody dilution was done in 3% bovine serum albumin (BSA) solution in DPBS (Sigma-Aldrich #A7979), 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and then blocked with blocking buffer solution (PBS/0.05% Tween 20 /3% non-fat milk (AmericanBio) /0.5% Bovine Serum Albumin (Sigma)) at room temperature for 2 hours ...
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bioRxiv - Biophysics 2023Quote: ... cells were blocked and permeabilized with blocking buffer (3% (w/v) bovine serum albumin (BSA, A3059, Sigma-Aldrich), 0.2% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... 3 PBS washes were performed and blocking was done with 2% Bovine Serum Albumin (Sigma Aldrich,#A7906-100G) and 5% goat serum (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... RGD peptide or as control GRADSP (RAD) peptide (SIGMA) was injected via Wharton’s duct cannulation (approximately 600 nmol of either peptide in 30 µl per lobe in PBS) ...
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bioRxiv - Genomics 2020Quote: ... a saturation step was performed with blocking solution (blocking reagent Sigma #11096176001 ...
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bioRxiv - Immunology 2022Quote: ... After blocking with IHC Select Blocking Reagent (Millipore, cat. 20773-M) at RT for 2 h ...
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bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Bioengineering 2021Quote: Sections were incubated in a permeabilization and blocking solution of 0.3% Triton X (A4975, PanREAC AppliChem) and 3% BSA (A7906, Sigma) in PBS for 24 hours at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: Sections were washed in 0.1M PBS solution before blocking by incubation with 3% normal donkey serum (NDS, Sigma; D9663), 0.2% PBS-Triton for 2 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a one-hour blocking step using 0.1% triton-X with 3% Bovine Serum Albumin (BSA, Sigma A7906) in PBS ...