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Citations for Millipore Sigma :
551 - 600 of 1332 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... and SARS-CoV-2 was inactivated with 1% Triton-X (Sigma-Aldrich, Burlington, MA) in PBS for 1 hour ...
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bioRxiv - Synthetic Biology 2020Quote: ... Primers were ordered as PAGE or desalted purified oligonucleotides (Table S1) from Sigma-Aldrich (St Louis, MO). Annealed oligos were quantified by BR ds DNA kit using Qubit spectrophotometer (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... Primer sequences are shown in Table S1 and were designed in SnapGene and ordered from Sigma Aldrich. Fragments for lentiviral vector construction were generated via PCR using Q5 polymerase (NEB M0491S ...
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bioRxiv - Immunology 2021Quote: ... Table S1) were individually loaded into a 25 mm stainless steel filter holder (Millipore Sigma, Burlington, MA). Purified dry N2 was blown through the filter holder for 15 min at a rate of 4.1 slpm based on estimates of the peak air flow rate experienced by a typical respirator mask during normal breathing ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with vehicle or 10% (v/v) normal human serum (Millipore, S1-100ML, lot# 2330486) in serum free media ...
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bioRxiv - Neuroscience 2023Quote: ... carvone (Od1) / Citral (Od2) for S1 session and limonene (Od1) / anethol (Od2) for S2 session (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: Samples were digested to nucleosides at 37°C overnight with Nuclease S1 from Aspergillus oryzae (Millipore Sigma), followed by dephosphorylation with FastAP alkaline phosphatase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tag was detected with peroxide–anti-peroxide complexes (P1291; Sigma). Detection was performed using the enhanced chemiluminescence procedure (ECL kit).
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bioRxiv - Developmental Biology 2019Quote: ... incubated with antibodies directed either against HA-tag (Sigma, 1:200) or VEGFC (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... anti-MYC (for MYC tag) (Sigma, St. Louis, MO, cat # C3956).
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bioRxiv - Plant Biology 2021Quote: ... and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... The GST tag was cleaved with thrombin (commercial thrombin, Sigma Aldrich), at 200 units per mL for 2h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-histidine tag 1:200× (Sigma-Aldrich, St. Louis, MO) and HRP-conjugated goat anti-mouse IgG 1:1,000× (Seracare Life Sci. ...
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bioRxiv - Biochemistry 2022Quote: ... the anti-Myc tag antibody clone 4A6 (Millipore, cat# 05-724), and the anti-RNA polymerase II antibody clone CTD4H8 (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG tag (Merck, Darmstadt, Germany /Sigma Aldrich, St. Louis, MO, USA), Spot tag (ChromoTek ...
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... and a mouse monoclonal anti-FLAG epitope-tag antibody (Sigma #F7425). For the detection of endogenous Pdlim5 ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Neuroscience 2023Quote: The StrepII–GFP tag was cleaved by HRV 3C protease (Novagen) added at 10 U/mg of BmGr9 overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Flag tag (F3165) and C14ORF43 (HPA003111 for WB) from Sigma-Aldrich. Monoclonal antibodies against TFII-D subunits were generous gifts from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... and validated by Western Blotting for 3xFLAG tag (Antibody M2, Sigma).
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Spike-transfected CEM NKR-Luc cells were stained with PKH26 kit according the manufacturers protocol (Sigma) prior to performing the ADCC assay ...
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bioRxiv - Biophysics 2020Quote: ... Cells were treated with a spike of 25 nM Tetramethylrhodamine ethyl ester perchlorate (TMRE, Sigma 87917) for 30 minutes before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...