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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... N’ -Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN, Sigma) was infused into the bath at 20-100 µm ...
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bioRxiv - Physiology 2020Quote: ... N’-tetrakis(2-hydroxypropyl) ethylenediamine (Sigma #122262) and 15% (W/V ...
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bioRxiv - Immunology 2023Quote: ... N’-bis(2-ethanesulfonic acid)] buffer (Sigma) for ten minutes at room temperature and blocked with cold normal donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma, 03450) in 50 mM HEPES were added on top of the hydrogels and incubated for 30 min at RT ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich, 03450) diluted in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma, catalog number 03450) in 50 mM Hepes] followed by 10 min UV-light activation ...
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bioRxiv - Biophysics 2020Quote: ... and 2 mg/ml N-(3-dimethylaminopropyl)-N′-ethylcarbodiimidehydrochloride (EDC, Sigma-Aldrich, 03450) in 50 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) ...
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bioRxiv - Neuroscience 2022Quote: ... buffered with 3 mM N-2-hydroxyethylpiperazine-N’-ethanesulfonic acid (HEPES, Sigma Chemical) and pH adjusted to 7.65 at 15°C ...
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bioRxiv - Microbiology 2022Quote: ... combined with 2 μL N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA, Sigma-Aldrich #394882) in a GC auto-sampler vial with a 200 μL glass insert ...
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bioRxiv - Biochemistry 2023Quote: Bicine: N,N-Bis(2-hydroxyethyl)glycine (Millipore Sigma, cat. No. B3876-25G)
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... with 2% foetal calf serum (FCS, Sigma-Aldrich, UK). Lymphoprep (d = 1.077 g mL−1 ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Cancer Biology 2019Quote: ... CUBIC reagent 1 was prepared as a mixture of 25 wt% N,N,N,N’-tetrakis (2-hydroxypropyl) ethylenediamine (Sigma-Aldrich, 122262), 25 wt% urea (Carl Roth ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleaved 3HB-SPYcatcher003 with a cysteine residue at the N-terminal was concentrated using Amicon 30K (Millipore), mixed with EZ-link Maleimide-PEG2-Biotin (Thermo A39261) ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma, Burlington, MA). After 19 h incubation at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25mM Hepes (N-[2-hydroxyethyl]piperazine-N0-[2-ethanesulfonic acid; Sigma]), supplemented with 10% heat inactivated FBS (GIBCO ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos and larvae in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Neuroscience 2019Quote: ... Formulation to dissolve radiprodil consisted of 2% N,N-dimethylacetamide (DMA, Sigma Cat# D5511)/10%propylene glycol (PG ...
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bioRxiv - Neuroscience 2022Quote: Trans-N,N′-(Cyclohexane-1,4-diyl)bis(2-(4-chlorophenoxy) acetamide (ISRIB, Sigma, SML0843) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2020Quote: ... and sheep serum (Sigma) was used ...
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bioRxiv - Immunology 2023Quote: ... 10 µM N-acetylcysteine (NAC) or 2 mM 2-desoxyglucose (2-DG, both Sigma) were added during cultivation ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the noradrenergic neuron specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4; Sigma, C8417) once at a dose of 50 mg/kg (i.p.) ...
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bioRxiv - Biophysics 2021Quote: ... Gelsolin TL40 (N-terminal cytoplasmic gelsolin, Hypermol, Germany) was covalently bound to carboxylated microspheres (0.9 µm diameter, Sigma Aldrich) with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in order to bind the +end of a single actin filament ...
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bioRxiv - Neuroscience 2020Quote: ... live TTX-treated neurons were incubated with either a mouse monoclonal anti-N-terminal GluA1 (1:40, Merk-Millipore) or GluA2 (1:30 ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mg/ml N-acetyl-l-cysteine (Sigma) and ITS liquid media supplement (100x ...
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bioRxiv - Immunology 2021Quote: ... 2 mM N-Acetyl-L-cysteine (Cysteine; Sigma), 10 mM Zinc sulfate heptahydrate (Zinc ...
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bioRxiv - Neuroscience 2021Quote: ... and 2% N-21 (Cat #SCM081, Millipore Sigma), plated at a density of 5 ganglia per well ...
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bioRxiv - Bioengineering 2021Quote: ... and N-methyl-2-pyrrolidone (NMP, Sigma-Aldrich) at a 1:1 volume ratio was used with the pneumatic atomiser and a tip size of 300 μm ...
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bioRxiv - Developmental Biology 2022Quote: ... N-acetylcysteine (2 mM) (Sigma, Cat#A9165-25G), Nicotinamide (20 mM ...
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bioRxiv - Biophysics 2020Quote: ... 2-(N-morpholino)ethanesulfonic acid (MES, M3671, Sigma), N,N’-methylen-bisacrylamide (BIS ...
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bioRxiv - Molecular Biology 2019Quote: SK-N-BE(2) cells (95011815, Sigma-Aldrich) were maintained in 1:1 medium composed of Ham’s F12 medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... and 2% N-21 (Cat #SCM081, Millipore Sigma), plated at a density of 0.5 ganglia per cloning cylinder ...
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bioRxiv - Cell Biology 2022Quote: ... N’-methylene bisacrylamide (2% wt/vol, Sigma-Aldrich) were mixed with distilled water to obtain gels of 10 kPa stiffness (Tse and Engler ...
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bioRxiv - Microbiology 2022Quote: ... SK-N-BE(2) cells (95011815, Sigma-Aldrich) were maintained in 1:1 medium composed of Ham’s F12 medium (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% FCS and 0.5µg/ml DAPI (Sigma Aldrich, Ref: D9542) and passed through a 40µm Falcon cell strainer ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...