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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with a MOI 0.002 (250 plaque forming units (PFU)) of SARS-CoV-2 for 1hr before overlaying with 1% methylcellulose- viscosity (4,000 centipoises) (Sigma- Aldrich, St. Louis, MO). Cells were incubated for 4 days at 37°C before fixing in 8% formaldehyde and stained with 1% crystal violet to visualize plaques ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3000 in PBST was then added and plates were incubated for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the glycoprotein bands were probed with 1 μg/ml Concanavalin A (Con A) peroxidase conjugate (Sigma-Aldrich, L6397). Signals were detected by electrochemiluminescence (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... incubated for 2 h with horseradish peroxidase (HRP)-conjugated secondary antibody (12-341 Rabbit anti-chicken RAC-HRP, Sigma-Aldrich) diluted 1:15000 in BSA-TBST ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibodies and the Chemiluminescent Peroxidase Substrate (Sigma-Aldrich, 34578) were used ...
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bioRxiv - Immunology 2020Quote: ADCP assays were performed using ExpiCHO-S target cells transiently transfected with SARS-CoV-2 S and fluorescently labeled with PKH67 Fluorescent Cell Linker Kits (Sigma Aldrich, Cat. Nr.: MINI67) as targets ...
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bioRxiv - Immunology 2020Quote: ... and 50 μl of horseradish peroxidase-Staphylococcal protein-A conjugate (Sigma-Aldrich) diluted 1:3000 was added ...
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bioRxiv - Molecular Biology 2021Quote: ... Horseradish peroxidase-conjugated with anti-rabbit HRP and anti-mouse HRP (Sigma) were the secondary antibodies used ...
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bioRxiv - Plant Biology 2019Quote: ... primary antibodies and secondary antibodies coupled to Horseradish Peroxidase (HRP, Sigma Aldrich) for protein detection on blots.
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bioRxiv - Cell Biology 2019Quote: ... Horseradish peroxidase–conjugated secondary antibody (anti-mouse) was obtained from Sigma-Aldrich. For Western blotting ...
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bioRxiv - Plant Biology 2021Quote: ... (2016) followed by a horseradish peroxidase conjugated rabbit secondary antibody (Sigma-Aldrich). Detection was carried out using the Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate kit and a Typhoon™ FLA 9500 (General Electric Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit horseradish peroxidase (HRP) (Sigma-Aldrich, A6154-5X1ML, 1:20 000) or anti-mouse HRP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... oxysporum strains towards tomato root exudate and horseradish peroxidase (HRP; Sigma - Aldrich) was measured using a quantitative plate assay as previously described (Turrà et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated with streptavidin-horseradish peroxidase and visualized using ECL (Millipore) and analyzed on Pxi (Syngene) ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were purchased from Sigma-Aldrich. Alexa Fluor-conjugated secondary antibodies used for immunofluorescence experiments were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2022Quote: ... Horseradish peroxidase conjugated anti-rabbit IgG (1:10000 v/v; Sigma-Aldrich) was used as a secondary antibody (1 h incubation ...
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bioRxiv - Genetics 2021Quote: ... goat anti-mouse IgG conjugated to horseradish peroxidase (1:10000; Sigma-Aldrich), and chicken anti-rabbit IgG conjugated to horseradish peroxidase (1:10000 ...
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bioRxiv - Microbiology 2022Quote: ... anti-β-actin antibodies conjugated with horseradish peroxidase (HRP) (Millipore Sigma (A3854)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using secondary polyclonal horseradish peroxidase-conjugated antibody (Sigma A0545), 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed with horseradish peroxidase-conjugated secondary IgG (1:5000) (Millipore) for 1h at RT ...
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bioRxiv - Biochemistry 2019Quote: ... Horseradish peroxidase-conjugated secondary antibodies (mouse and rabbit) were obtained from Sigma. For developing the blot ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-mouse or goat anti-rabbit horseradish-peroxidase-conjugated antibodies (Sigma) were used ...
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bioRxiv - Biochemistry 2021Quote: ... horseradish peroxidase-conjugated goat anti-mouse antibody (Sigma, St. Louis, MO, USA) was used at 0.8 μg/ml or horseradish peroxidase-streptavidin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... conjugated to horseradish peroxidase and Sigma Fast o-phenylenediamine dihydrochloride (Sigma-Aldrich). The optical density was determined at 450nm using a Victor X3 plate reader (Perkin Elmer ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were visualized using Immobilon Western chemiluminescent horseradish peroxidase substrate (Millipore), and the bands were analyzed by ImageJ software.
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase– conjugated secondary antibodies (mouse and rabbit) were obtained from Sigma. For Western blotting ...
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bioRxiv - Cell Biology 2022Quote: ... anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of TMB/E horseradish peroxidase (HRP) substrate (Millipore Sigma, ES001) were added to each well ...
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bioRxiv - Neuroscience 2022Quote: ... which are horseradish peroxidase (HRP)- conjugated (1:3000 or 1:10,000-Sigma). The blots were developed using Clarity ECL reagent in Chemidoc gel apparatus (Biorad ...
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bioRxiv - Microbiology 2023Quote: ... Anti-IgG secondary antibodies conjugated with Horseradish Peroxidase (HRP) (Sigma-Aldrich®) at a dilution of 1:5.000 were applied after five PBS-T washes ...
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bioRxiv - Molecular Biology 2023Quote: ... and the horseradish peroxidase-coupled secondary antibody (HRP) (Sigma, #A9044 and #A9169). The signals were revealed using an electrochemiluminescence reagent (ClarityWestern ECL ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies used: anti-FLAG-horseradish peroxidase (HRP, Sigma-Aldrich, A8592, 1:5000), anti-β-tubulin (Proteintech ...
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bioRxiv - Microbiology 2023Quote: ... while horseradish peroxidase-conjugated goat anti-mouse antibody was acquired from Millipore Merck KGaA (Darmstadt ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-rabbit horseradish peroxidase (HRP) secondary (#NA9340V, Sigma-Aldrich, St. Louis, Missouri) at 1:5000 dilution was prepared in 5% fat-free milk and added to membrane for one hour at room temperature with shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... horseradish peroxidase-linked anti-rabbit IgG (Sigma-Aldrich, A9169-2ML, 1:5,000), horseradish peroxidase-linked anti-guinea pig IgG (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... or rat horseradish peroxydase-conjugated antibody against HA-Tag (Clone 3F10, Sigma-Aldrich). Quantifications were performed by means of the luminescence image analyzer Image Quant Las 4000 mini and Image Quant software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... containing 2% FCS (Sigma Aldrich), 0.1 mg/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 100 μg/ml gentamycin (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked in 2% sheep serum (Sigma, St. Louis, MO), 2 mg ml−1 bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The following day plates were washed three times with TBS-T and incubated for 2 hours at room temperature with secondary antibody horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917), diluted to 1 in 1,000 in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... pTAC-MAT-Tag-2 (Sigma-Aldrich) was PCR-amplified with primers pTAC-Gbs-for/rev (Table S9) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-rabbit horseradish peroxidase (HRP) (diluted at 1:20,000; Sigma-Aldrich, Switzerland) was used as the secondary antibody against the anti-Hcp primary antibody ...