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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Either 15 μg of nanobody Fc plasmid or 1.5 μg of nanobody Fc plasmid and 13.5 μg of ssDNA (Sigma, D7656) were combined with 45 μg PEI (Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... We added a spike-in of digested unmethylated lambda phage DNA (Sigma Aldrich) to each sample at a concentration of 0.1% of the sample concentration ...
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bioRxiv - Microbiology 2023Quote: ... an internal spike-in standard of 100mM chenodeoxycholic acid-D4 (CDCA-D4, Sigma) was added to initial culture supernatants as a normalization reference ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells were treated with tetracycline hydrochloride (1 µg/ml; T7660, Sigma-Aldrich) for 48 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent immunoblotting against either the FLAGepitope tag or TWIN-Strep epitope tag (Anti-FLAG M2, Sigma-Aldrich; THETM NWSHPQFEK antibody, GenScript).
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bioRxiv - Microbiology 2019Quote: Bacterial strains used in this study (Table S2) were grown in Luria Bertoni (LB) (Sigma), brain heart infusion (BHI ...
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bioRxiv - Genomics 2019Quote: ... Drosophila S2 cells from the DGRC were grown in Shields and Sang M3 (Sigma S3652) media supplemented with bactopeptone (BD Biosciences 211677) ...
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bioRxiv - Cell Biology 2019Quote: For acute inhibition of Cdk1 in S2 and U2OS cells the Cdk1 inhibitor RO3306 (Sigma) was added in metaphase or at anaphase onset ...
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bioRxiv - Biochemistry 2020Quote: ... S2 cells were allowed to settle on coverslips treated with Poly-L-Lysine (Sigma-Aldrich). After gentle washing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primers were designed using Primer3 (version 4.0.0) and were synthesized by Sigma (Supplementary information, S2). To ensure there was no spurious amplification ...
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bioRxiv - Biophysics 2022Quote: Insect S2 cells from the Fehon Lab (UChicago) were maintained in Schneider’s insect media (Sigma) with insect media supplement (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... was generated by transfecting 25 ml of S2 cells grown in Ex-Cell420 medium (Sigma) with 10 % (v/v ...
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bioRxiv - Synthetic Biology 2020Quote: ... Oligonucleotide primers used in this study (Supplementary Table S2) were purchased from Sigma-Aldrich (USA). Plasmid DNA was isolated with QIAprep Spin Miniprep kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Cancer Biology 2022Quote: ... using gene-specific primers (Table S2) and FastStart Universal SYBR Green master mix (Millipore-Sigma). Reactions were run in duplicate and relative Ct values were normalized and calculated independently using the –ΔΔ Ct method for the expression of the housekeeping genes HPRT1 and RPL13A (all primers are listed in Table S1).
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Neuroscience 2022Quote: ... wells were washed three times using H2O 0.05% Tween20 and binding of NMDAR antibodies to the immobilized N1-N2B-Fc was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Cell Biology 2020Quote: ... and S-tag (EMD Millipore, MAC112, WB: 1:5000). Rat monoclonal antibody ...
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bioRxiv - Physiology 2020Quote: ... Plasma TAG was measured with a colorimetric assay (Sigma).
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Neuroscience 2019Quote: ... anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich), anti-tubulin # T8660 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to Flag-tag (F3165) were purchased from Sigma. Antibodies to pT308-Akt (ab38449 ...
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tag (M2) and α-Tubulin (T5168) from Sigma, RFP (600-401-379 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...
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bioRxiv - Microbiology 2019Quote: ... The GST tag was cleaved with PreScission Protease (Millipore) and proteins were dialyzed overnight in 50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... against a primary antibody (GST-tag Monoclonal antibody, Novagen), which is attached to GST-tagged TF proteins (Figure S4).
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by thrombin protease (Sigma) in cleavage buffer (20 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Biochemistry 2021Quote: ... GST tag was cleaved by incubating with thrombin (Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The FLAG tag oligonucleotide was synthesized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 1).
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bioRxiv - Genetics 2020Quote: ... rabbit αMyc Tag IgG (1:3000; #06-549, Millipore), rabbit αHSF2BP R2 (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... and S-tag from MBP-enhanced vector pTriEx4 (Novagen); the second insert contained CL Affinity Tag followed by a 90bp linker taken from pl21 plasmid (TriAltus) ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Cell Biology 2023Quote: ... TAMRA-alkyne tag (1:5000; Sigma Aldrich, Cat# 900932), TCEP solution (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibody for Flag-Tag (A8592) was ordered from Sigma.
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bioRxiv - Cell Biology 2019Quote: ... and 10% Fetal Calf Serum (FCS, Sigma-Aldrich). The CEM T cell line was provided by the ATCC ...