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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... LPS (E. coli O111:B4, Sigma) was dissolved in PBS to make a stock solution then sonicated in a water bath for 10 min prior to addition to cell suspensions ...
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bioRxiv - Biochemistry 2023Quote: ... and expressed in BL21 Rosetta 2 (DE3) pLysS Escherichia coli (E. coli) cells (Novagen). All plasmids were verified by Sanger sequencing (OSU Shared Resources) ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike expressing expi293F cells were stained with PKH26 (Sigma-Aldrich, St-Louis, MO). Cells were then washed with and resuspended in R10 media ...
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bioRxiv - Cancer Biology 2019Quote: E coli Rosetta (DE3) pLysS cells (Novagen) transformed with pOPIN-B plasmids encoding wtGATA3 and neoGATA3 were grown in autoinduction media supplemented with 50 µg/ml kanamycin and 34 µg/ml chloramphenicol for 8 h at 37 °C followed by 21 h at 20 °C ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Biochemistry 2022Quote: ... 50ng/mL LPS (E. coli O111:B4, Sigma) and 10ng/mL IFN-γ (R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... ampicillin (Sigma; 100 μg/mL for E. coli), gentamicin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: LPS (Sigma Aldrich, E. coli, serotype 0111:B4), was prepared by dissolving 0.3 mg of LPS in 1ml of PBS (Phosphate buffer saline) ...
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bioRxiv - Bioengineering 2022Quote: ... and LPS (lipopolysaccharides from E. coli; Sigma L8274); HEK-293Ta showed the strongest response to TNFα and were thus selected as responder cells in the co-culture assay (Fig ...
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bioRxiv - Microbiology 2022Quote: ... or tRNA (from E. coli MRE 600, Sigma) was incubated with PT mutants ...
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bioRxiv - Cancer Biology 2021Quote: LPS derived from Escherichia coli (E. coli) was from Sigma-Aldrich Canada (Oakville ...
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bioRxiv - Microbiology 2023Quote: ... coli strain BL21 (DE3) (E. coli BL21, EMD Millipore, Cat#69450). F ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Immunology 2020Quote: ... injection of either PBS or 2 mg/kg LPS (Sigma (E. coli 055:B5)) dissolved in PBS ...
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bioRxiv - Biochemistry 2023Quote: Escherichia coli (E. coli) Rosetta2(DE3)pLysS cells (Novagen, cat. no. 71403) were transformed with pET28a-MDA5ΔL2-His6 and grown in 2xTY medium to OD600 0.4-0.6 at 37°C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 labelled bacteria (E. coli, Sigma-Aldrich) and yeast (S ...
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bioRxiv - Immunology 2021Quote: ... LPS (E. coli serotype O55:B5, Sigma, 10ng/ml), CpG ODN 1826 (TIB MOLBIOL ...
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bioRxiv - Neuroscience 2023Quote: LPS (E. coli, O111:B4 serotype, Sigma Aldrich, UK) was dissolved at 0.5 mg/ml in sterile 0.9% saline ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... 2-mercaptoethanol (Millipore, ES-007-E), 1x penicillin/streptomycin (Millipore ...
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Neuraminidase inhibitors rewire neutrophil function in vivo in murine sepsis and ex vivo in COVID-19bioRxiv - Immunology 2022Quote: ... LPS (1 µg/mL, E. coli 0127:b8, Sigma-Aldrich), LPS plus Oseltamivir or LPS plus Zanamivir ...
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bioRxiv - Microbiology 2022Quote: ... 0.2 U Pyrophosphatase (E. coli, MFCD00131379 from Sigma-Aldrich®), 0.127 mg.ml−1 MtATPS ...
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bioRxiv - Neuroscience 2022Quote: ... or lipopolysaccharide (LPS, Sigma-Aldrich, E. coli, O111:B4, L4391) dissolved in PBS at a dose of 5 mg/kg (cohort 1 ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Immunology 2019Quote: ... or LPS Ultrapure (standard LPS, E. coli 0111:B4; Sigma-Aldrich); or with the negative control for cell stimulation (the medium) ...
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bioRxiv - Immunology 2020Quote: ... Purified E coli flagellin and LPS (Sigma, St Louis, Missouri, USA) were used as positive controls for HEK-Blue-mTLR5 and HEK-Blue-mTLR4 cells ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... we diluted LPS (from serotype 055:B5 E. coli; Sigma-Aldrich) to 0.5mg/mL in a sterile physiological saline (Ringer’s solution ...
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bioRxiv - Immunology 2020Quote: ... or LPS (0.2μg ml−1, E. coli O55:B5 Sigma-Aldrich) in the presence of M-CSF (10ng ml−1 ...
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bioRxiv - Microbiology 2020Quote: Overexpression of was performed by using Escherichia coli (E. coli) Rosetta (DE3) pLysS cells (Novagen) harboring the E ...
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bioRxiv - Biochemistry 2024Quote: ... for 2 h before stimulation with 100 ng/mL lipopolysaccharide (from E. coli O55:B5, Sigma Aldrich), with four biological replicates per condition ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Bioengineering 2023Quote: mTmG mice were treated with 100 µg Escherichia coli (E. coli, serotype O55:B5, Sigma-Aldrich) lipopolysaccharide (LPS ...
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bioRxiv - Biochemistry 2022Quote: ... cells were incubated with 50ng/mL LPS (E. coli O111:B4, Sigma) and 30ng/mL IFN-γ (R&D Systems).
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bioRxiv - Immunology 2020Quote: We used LPS (from E. coli serotype O55:B5, Sigma, Rehovot, Israel) or human TNFα (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... ii) 50 ng/ml LPS (E. coli 0111:B4, Sigma-Aldrich L288) for 2 days then with 20 ng/ml INF-γ (ImmunoTools ...
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bioRxiv - Neuroscience 2023Quote: Lipopolysaccharides (LPS, E. coli, O55:B5, 3×106 EU/mg, Sigma #L2880) was dissolved in 0.9% sterile saline (NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ng/mL lipopolysaccharide (LPS) (E. coli 0111:B4) (Sigma-Aldrich, L3024) and 10 ng/mL recombinant mouse interferon-γ (IFN-γ ...
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bioRxiv - Microbiology 2023Quote: ... LPS (standard LPS, E. coli 0111: B4; Sigma-Aldrich, 500 ng/mL) plus Nigericin (tlrl-nig ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... the whole operon of FhuCDB was cloned from Escherichia coli (E. coli) genome into a pET15b (Novagen) vector with a His-tag and a thrombin digestion site on the N-termini of FhuC ...