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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing medium was collected 46 and 65 hours post transfection and was filtered through 0.45 μM Millipore low-protein binding filter units (Millipore SCHVU02RE). Viral supernatant was concentrated by first using low speed centrifugation through 100 kDa MW cutoff Millipore Centricon 70 Plus cartridges to condense volume to < 4mL ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Developmental Biology 2021Quote: ... As control for iodide uptake and protein-binding measurements cells were also treated with 30µM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 µM methimazole (TPO inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Cancer Biology 2022Quote: ... Hydrogels were made permissive for protein binding by incubating them in 500 µl of Sulfo-SANPAH solution [0.2 mg/ml Sulfo-SANPAH (Sigma, 803332) and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed again with 800 μl binding buffer three times and the bound proteins were eluted with the binding buffer supplemented with 10 mM D-desthiobiotin (Sigma) and analyzed with SDS PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... the virus-containing supernatant was harvested by gentle pipetting and filtered with a low protein binding syringe filter (0.22 μM, EDM Millipore # SLGV033RB). The virus was frozen at -80 °C for long-term storage or transduced directly into the desired cells.
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bioRxiv - Immunology 2023Quote: ... Protein was harvested from cell culture supernatants and purified by binding to a nickel-nitrilotriacetic acid (Ni-NTA) column (Novagen) followed by elution with 1M imidazole containing 0.2% octyl β-D-glucopyranoside ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were then blocked with 300 μL of blocking buffer (1% BSA and 10% bovine calf serum (BCS) (Sigma) in PBS at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2020Quote: ... aureus binding to fibronectin and fibrinogen was determined using a semi-quantitative adherence assay on 96-well tissue culture plates (Sigma-Aldrich, USA). Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6 ...
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bioRxiv - Physiology 2021Quote: ... Insulin was measured by ELISA (EZRMI-13K Sigma-Aldrich - Rat/Mouse - Insulin ELISA) and the hepatic and skeletal muscle glycogen contents of fed rats (Lo et al ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Solubilized proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich), pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with 5 mM dithiothreitol (DTT) (Sigma-Aldrich) for 30 min at 55 °C and then alkylated by the addition of 10 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Purified 5-HT2C protein immobilized on nickel agarose beads (Sigma) was incubated with a given marker ligand at 2 nM mixed with each test compound (aporphine ligands ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated by adding 5-sulfosalicylic acid (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Immunology 2023Quote: ... Bound proteins were reduced with 5 mM DTT (Sigma-Aldrich) in 50 mM TEAB (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were reduced with 5 mM 5-Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma-Aldrich) for 2 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 15 minutes at −20°C and washed again with PBS after which aspecific antibody-binding was blocked for 2 hours in 5% bovine serum albumin (BSA, Sigma-Aldrich). Organoids were incubated overnight at 4°C with the primary antibodies (mouse anti-acetylated α-tubulin (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... BCA protein Assay Kit (Sigma-Aldrich) was used to quantify proteins in all the fractions and 30 µg of proteins per fraction were separated by SDS-PAGE for immunoblot.
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Neuroscience 2020Quote: ... The filtrate was transferred to a low-protein binding tube and 20 equivalents of dopamine (final concentration: 2.8 mM) (Sigma-Aldrich, H8502) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Developmental Biology 2022Quote: ... As control for iodide uptake and protein-binding cells were also treated with 30mM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 mM methimazole (TPO inhibitor ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the phage suspensions were further filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts), diluted and plated as indicated in the previous paragraph to verify the uniformity of the plaques ...
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bioRxiv - Neuroscience 2020Quote: ... Blood MeS markers were then quantified using an ELISA kit (insulin: EZRMI-13K, Millipore; glucose) and colorimetric assays (triglyceride ...
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bioRxiv - Immunology 2022Quote: ... Release of IL-2 into supernatant was measured using a commercial ELISA kit (Sigma-Aldrich).
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ARID1a protects against steatosis and insulin resistance via PPARalpha-mediated fatty acid oxidationbioRxiv - Cell Biology 2019Quote: ... Insulin plasma levels were determined using mouse insulin ELISA kit (Millipore, St. Charles, MO, US). The levels of the serum inflammatory indicators were measured by ELISA (BD Bioscience ...
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bioRxiv - Physiology 2021Quote: Plasma FGF21 (pg/ml) was measured using a human FGF21 ELISA kit (Millipore, Billerica, MA). In Experiment 2 ...
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bioRxiv - Immunology 2022Quote: ... Hb OD directly correlated to Hb concentration quantified by ELISA (Hemoglobin Assay Kit, Sigma Aldrich) (r2 = 0.9659 ...
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bioRxiv - Physiology 2019Quote: ... Serum insulin and testosterone levels were measured using ELISA kit purchased from Millipore (#EZRMI-13K) and Crystal Chem (#80552) ...