Labshake search
Citations for Millipore Sigma :
151 - 200 of 7341 citations for Recombinant Sheep IL22 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
-
bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
-
bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
-
bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
-
bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
-
bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
-
bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
-
bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
-
bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
-
bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
-
bioRxiv - Neuroscience 2022Quote: ... After blocking for 90 min with 5% sheep serum (Sigma-Aldrich) at room temperature ...
-
bioRxiv - Physiology 2020Quote: ... biotinylated donkey anti-sheep antibody (1:200, Sigma, cat no. AP184B), streptavidin-peroxidase conjugate (1:200) ...
-
bioRxiv - Genetics 2019Quote: ... embryos were incubated in working strength sheep anti-digoxigenin rhodamine (Millipore) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... or sheep’s blood (Hemostat DSB100) supplemented with 1mM ATP (Sigma A6419). Meals were warmed to 45°C before being used in the assay ...
-
bioRxiv - Cell Biology 2021Quote: ... for sheep antibodies or 3% (w/w) BSA (Sigma-Aldrich, A3059) for other antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma) was then added for 1 h ...
-
bioRxiv - Biochemistry 2021Quote: ... affinity purified Ubxn7 antibodies or IgG from sheep serum (15131, Sigma);
-
bioRxiv - Biochemistry 2021Quote: ... coupled to antibodies against Ubxn7 or nonspecific sheep IgGs (15131, Sigma), with two rounds of 1 h incubation at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Digoxigenin polyclonal antibody (Sheep, SKU: 11333089001) was obtained from Sigma Aldrich and digoxigenin monoclonal antibody (Clone # 611621 ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-rabbit anti-chicken and anti-sheep were purchased from Sigma.
-
bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
-
bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
-
bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
-
bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
-
bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
-
bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
-
bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
-
bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
-
bioRxiv - Developmental Biology 2019Quote: ... containing L-15 medium and sheep serum (Sigma-Aldrich, 10% v/v) as blocking solution ...
-
bioRxiv - Neuroscience 2021Quote: ... The primary antibodies were anti-TPH (Millipore, sheep polyclonal, 1:1000 dilution) and anti-TH (Millipore ...
-
bioRxiv - Biochemistry 2023Quote: ... and sheep secondary antibodies for immunoblotting and immunofluorescence were obtained from Sigma.
-
bioRxiv - Neuroscience 2023Quote: ... Females were fed sheep blood (Hemostat DSB100) supplemented with ATP (Sigma A6419) at a final concentration of 1 mM using a Glytube membrane feeder (58) ...
-
bioRxiv - Biochemistry 2024Quote: ... and sheep secondary antibodies for immunoblotting and immunofluorescence were obtained from Sigma.
-
bioRxiv - Neuroscience 2020Quote: Recombinant Fetuin-A (Sigma; SRP6217) was diluted in 0.9% saline vehicle solution ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant PAK1 (Millipore, 14-927) and agitated at 30°C for 1 hr ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant bacterial sphingomyelinase (S7651, Sigma); 2-bromopalmitate (320-76562 ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse recombinant IFN-β (Millipore) diluted in DPBS with 0.1% Bovine serum albumin BSA (Roche ...
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
-
bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
-
bioRxiv - Genetics 2019Quote: ... 15 U recombinant DNase1 (Sigma) and 10 U DNA polymerase I (NEB) ...
-
bioRxiv - Cell Biology 2021Quote: ... and recombinant DNAse I (Sigma) in MACS buffer (PBS/2mM EDTA/ 0.5% bovine serum albumin (BSA) ...
-
bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
-
bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
-
bioRxiv - Microbiology 2021Quote: Recombinant mouse adiponectin (Sigma, #SRP3297) and GFP protein (Sino Biological ...
-
bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...