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Citations for Millipore Sigma :
51 - 100 of 7286 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were created using the pET expression system (Novagen). The codon-optimized typical faeG gene of the original K88 (K88ac variant ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... active recombinant proteins were purchased from Sigma (SRP0364, P0066, respectively). Recombinant purified human MASTL protein was used as a substrate and was from Abnova (H00084930) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were expressed in either BL21 or Rosetta (Novagen) cells ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... LPA and recombinant Src protein were purchased from Sigma-Aldrich, while recombinant human IDO1 protein was obtained by Giotto Biotech ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Cell Biology 2021Quote: ... antibody-bound fraction was immunoprecipitated for 90 minutes at 4°C using 25 µL slurry salmon sperm protein A agarose beads (Millipore). Unbound proteins were removed by washing the beads 5 times in wash buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 × protease inhibitor cocktail] and precleared by incubation with 50 μL Protein-A agarose beads/Salmon sperm DNA (Millipore) at 4 °C for 1 h ...
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bioRxiv - Genomics 2020Quote: ... 0.4mg/mL salmon testes DNA (Sigma-Aldrich, Catalogue #D7656)) ...
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bioRxiv - Pathology 2019Quote: ... 0.1 mg/mL salmon sperm DNA (Sigma-Aldrich, D7656), and 10 mg/mL dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Pathology 2019Quote: ... 0.1 mg/mL salmon sperm DNA (Sigma-Aldrich, D7656), and 10 mg/mL dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.5 µg/µl salmon sperm DNA (Sigma-Aldrich). The HSE-containing oligonucleotides 5’-biotin-TCGACTAGAAGCTTCTAGAAGCTTCTAG-3’ and 5’-CTAGAAGCTTCTAGAAGCTTCTAGTCGA-3’ (Vuori et al ...
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bioRxiv - Genomics 2022Quote: ... sheared salmon sperm DNA 20μg (Sigma-Aldrich, Dorset, UK), SDS (sodium dodecyl sulphate ...
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bioRxiv - Bioengineering 2019Quote: ... either salmon sperm (Thermo) or Poly dI-dC (Sigma) were used as non-specific competitor DNA in the subsequent washing steps ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pH7) containing 100 µg/mL salmon sperm DNA (Sigma) for 2 h at 60° C ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.4mg/mL salmon testes DNA (Sigma-Aldrich, Catalog #D7656)) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of protein was subjected to a pre-clearing step by incubating samples with prewashed salmon sperm DNA/protein-A agarose beads (Millipore Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... 5% sheared salmon sperm DNA (Sigma, St. Louis, MO, USA), 0.3% yeast tRNA (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Commercially available Salmon Sperm ssDNA was obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2019Quote: ... Hydroxyurea (H8627) and salmon PROTAMINE (P4005) were obtained from Sigma and cycloheximide (239764 ...
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bioRxiv - Cell Biology 2022Quote: ... and 30 μg of salmon sperm DNA carrier (Sigma Aldrich). Then cells were electroporated with a BioRad Gene Pulser at 950 μF and 240 V using 4-mm width cuvettes ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 mg ml–1 salmon sperm DNA (Sigma-Aldrich, D7656) and 10 mg ml–1 dextran sulfate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Salmon sperm DNA (Sigma-Aldrich, Saint Louis, MO, Product #D1626) and Mucin (Sigma-Aldrich ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...