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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Recombinant Rabbit TNF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Biochemistry 2024Quote: Recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Molecular Biology 2021Quote: ... and TNF-α using Milliplex Mouse Th17 Luminex kit (EMD Millipore) on a MagPix Luminex instrument ...
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bioRxiv - Physiology 2022Quote: ... Tumor necrosis factor alpha (TNF-α, 20 ng/mL; Sigma-Aldrich) and cycloheximide (chx ...
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bioRxiv - Bioengineering 2021Quote: ... TNF-α (0.5 or 1 µg/mL, Sigma Aldrich, Cat# SRP3177) or lipopolysaccharides (LPS ...
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bioRxiv - Neuroscience 2021Quote: ... or TNF-α and IFN-γ (10 ng/mL; Sigma-Aldrich). In a second set of experiments D3 cells in triplicate wells were treated with 200 μl of the following ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant MejAgo protein expression and Ni-affinity chromatography purification procedures were performed according to user manual(Novagen). In perticular ...
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bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-protein kinase C-α (PKCα) (Sigma, P4334); rabbit anti-VSX2 (Chx10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit anti-Protein A antibodies (Sigma Cat. No P3775) was used for ChIP at a final concentration of 20 μg/ml of immunoprecipitate (IP) ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Lyophilized cytokines (TNF-α, IL-1α, C1q) were obtained from Sigma-Aldrich, resuspended in molecular biology grade water to make stock solutions ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Cytoplasmic interacting protein 1 (Millipore-Sigma, Cat # Ab6046), rabbit anti-β-Catenin polyclonal antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Sftpc (1:100, anti Pro Surfactant protein, rabbit, AB3786 Millipore), Ki67 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-microtubule associated protein-2 (MAP2) (Millipore Sigma AB5622), and mouse anti-TUBB3/β3 tubulin (Novus Biologicals ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...