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Citations for Millipore Sigma :
201 - 250 of 7537 citations for Recombinant NRG4 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... GST-fusion proteins were purified by loading total bacterial lysate on a preequilibrated glutathione Sepharose column (Sigma, Germany) using fast performance liquid chromatography system (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The mouse anti-GST antibody (Sigma) was used to detect the GST ...
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bioRxiv - Neuroscience 2021Quote: ... and GST (Sigma G7781, 1:3,000). To visualize GST signal ...
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bioRxiv - Cell Biology 2022Quote: ... or GST-bind (EMD Millipore; 70541) resin for 2 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... coli using GST-bind resin (Novagen) according to standard protocols ...
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bioRxiv - Cell Biology 2022Quote: ... and GST (Millipore Sigma #05-782). We also used our own antibody against Xenopus TOPBP1 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-GST mAb (1:2000, Sigma), anti-α-tubulin mAb (1:3000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GST (1:5,000, Sigma A-7340), karyopherin (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein of cell extracts was determined by Bradford method and GST□BHMT was precipitated with glutathione agarose (Sigma). Precipitates were then mixed in SDS□PAGE sample buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Molecular Biology 2019Quote: ... anti-Rpb4 and GST-HRP (A3740, SIGMA).
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... anti-GST rabbit 1/100,000 (G7781, Sigma), anti-MBP mouse 1/80,000 (E8032S ...
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bioRxiv - Cell Biology 2021Quote: a-GST (mouse monoclonal IgG1, Sigma #SAB4200237)
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bioRxiv - Plant Biology 2020Quote: ... 1:10000 for anti-GST (SIGMA, USA), 1:5000 for anti-HA (SIGMA ...
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bioRxiv - Biochemistry 2021Quote: ... in place of the GST gene (Novagen).
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bioRxiv - Cell Biology 2019Quote: ... and GST (1:10,000, Sigma A-7340). Appropriate horseradish peroxidase-labeled secondary antibodies (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-GST antibody (Sigma Aldrich, 06-332) was added diluted 1:1,000 in PBS-T with 3% BSA and incubated with gentle agitation in RT for 1 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 1:5,000 mouse anti-GST (Sigma SAB4200237); 1:2,000 rabbit anti-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biochemistry 2024Quote: ... a primary anti-GST (Sigma, 1:3,000) was used to detect bound PI(4,5)P2-grip protein.
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...