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Citations for Millipore Sigma :
251 - 300 of 9844 citations for Recombinant Mouse Vtcn1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human insulin was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibiotic-antimitotic solution ...
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bioRxiv - Biochemistry 2021Quote: ... Bovine erythrocyte ubiquitin and recombinant hsNEDD8 were purchased from Sigma-Aldrich (U6253) and BostonBiochem (UL-812 ...
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml of recombinant human leukemia inhibitory factor (LIF, EMD Millipore) and 1 mM Valproic acid (Wako) ...
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bioRxiv - Bioengineering 2020Quote: Recombinant human epidermal growth factor (EGF, 5 ng/mL; Sigma-Aldrich, E9644) was used as an invasion promoter and recombinant human nodal (250 ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant VEGF (V7259) and TGF-β1 (100-21) was purchased from Sigma and PeproTech ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The Red/ET recombinant enzyme was induced by L-arabinose (Sigma-Aldrich) and a temperature shift from 30°C to 37°C ...
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bioRxiv - Physiology 2020Quote: ... then injected with 0.75 IU/kg human recombinant insulin (I2643, Sigma Aldrich) at ZT6 (time “0 minutes”).
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bioRxiv - Biochemistry 2021Quote: Human recombinant SRPK2 (0.4 ug/reaction; EMD Millipore 14-666; Glu46-end) and SRSF2 (0.8 ug/reaction ...
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bioRxiv - Immunology 2021Quote: ... were coated with 100 ng/well recombinant human ACE2 (hACE2) (Sigma-Aldrich) in PBS ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 10 nM recombinant TeNT–light chain (Sigma–Aldrich) for 16 h in a CO2 incubator ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 400ng/mL of human recombinant leptin (Sigma #L4146). In leptin neutralization studies ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Microbiology 2021Quote: ... and 40 ng/mL bFGF (basic recombinant human fibroblast growth factor; Sigma) in flasks coated first with 50 µg/mL poly-L-ornithine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). 293T cells were cultured in DMEM supplemented with 10% FCS.
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bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). ESCs were cultured on plates which were pre-coated with 0.1% gelatin ...
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bioRxiv - Genetics 2020Quote: ... 10 ng/μL recombinant fibroblast growth factor basic (βFGF; Sigma-Aldrich, #F0291), 10 μg/μL Heparin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Biochemistry 2021Quote: Limited proteolysis experiments were conducted with recombinant human cathepsin S (Milipore-Sigma) diluted in phosphate-citrate buffer at pH 5.6 and 37 °C(21) ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were digested overnight at 37 °C with recombinant trypsin (Trypzean, Sigma) (enzyme ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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bioRxiv - Immunology 2021Quote: ... Recombinant Wnt5A (Cat no.- 10UG 645-WN-010 R & D, GF146 Millipore) and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... eluted in water and aliquoted for later use with recombinant Cas9 (Sigma). Oligos used for sgRNA production are listed in Extended Data Table 4.
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... PANC0813 medium was supplemented with 10units/mL human recombinant insulin (Sigma-Aldrich), and MHHNB11 medium was supplemented with MEM Non-Essential Amino Acids (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was digested by adding 10 U DNase I recombinant (Sigma) and incubating for one additional hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG ...