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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were dissected and immunostained as described previously.77 Dissected and fixed tissue was incubated overnight at 4°C with anti-Flag (mouse, 1:200; Sigma F1804) or anti-HA (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... Then the tissue was incubated overnight at 4°C in a mouse monoclonal anti-chondroitin sulfate (C8035; Sigma-Aldrich; 1:500) and a rabbit polyclonal anti-PV (ab11427 ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Cell Biology 2020Quote: ... 1% glycerol) containing protease inhibitors (cOmplete Protease Inhibitor Cocktail (#11697498001, Sigma, Austria), yeast protease inhibitor cocktail (yPIC ...
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bioRxiv - Developmental Biology 2021Quote: ... plus two inhibitors (KSOM+2i): 1 μM MEK inhibitor PDO325901 (Sigma-Aldrich) and 3 μM GSK-3 inhibitor CHIR9902 (Cayman Chemicals ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40) that contains protease inhibitors and PhosSTOP inhibitors (Sigma-Aldrich). Cells were then centrifuged at 14,000 g for 10 min at 4°C to remove cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... and 1/100 diluted phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2, Sigma-Aldrich). Samples were centrifuged at 20,000 x g for 20 minutes at +4°C and the supernatant was collected for further use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml recombinant human long-range IGF-1 (Sigma-Aldrich, USA), 5 ng/ml recombinant IL-6/soluble IL-6R fusion protein ((Fischer et al. ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was simultaneously incubated in mouse anti-NeuN (Millipore, Danvers, MA; RRID: AB_2298772) diluted 1:2000 in blocking serum.
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bioRxiv - Developmental Biology 2022Quote: ... Mouse tissue slides were mounted using Fluoromount aqueous mounting medium (Sigma-Aldrich, F4680) and were stored at 4°C until analyzed ...
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bioRxiv - Cell Biology 2023Quote: Cells or mouse tissues were lysed with RIPA buffer (Sigma-Aldrich, 20-188) supplemented with combinations of Protease/Phosphatase inhibitors (APExBIO) ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 1× PBS and 1× protease inhibitor cocktail (Sigma-Aldrich) and probe tip sonicated for three cycles for 20 s and intervals of 30 s on ice 22 ...
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bioRxiv - Systems Biology 2021Quote: ... 1 μM Mek 1/2 inhibitor PD0325901 (Sigma, PZ0162), 0.1 mM LIF ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 1% protease and 1% phosphatase inhibitor cocktails (Sigma). Protein samples were quantified using the Bradford assay reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... and 1× Phosphatase Inhibitor Mixtures 1 and 2 (Sigma)] ...
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bioRxiv - Neuroscience 2024Quote: ... 1% triton-X and 1:10 protease inhibitor (Sigma Fast inhibitor tablet ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... and then homogenized by sonication in lysis buffer containing 1% protease inhibitors and 1% phosphatase inhibitor (Sigma). The protein lysates containing 20 micrograms of protein were separated in 4-15% polyacrylamide gel (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... anti- HIS from Novagen (70796-3), anti-TGN38 from BD Transduction (610898) ...
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bioRxiv - Biochemistry 2019Quote: His-select Cobalt affinity beads (Sigma) were equilibrated in binding buffer (40 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-His (Sigma-Aldrich, H1029, RRID:AB_260015), anti-Strep (IBA GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His antibody (EMD Millipore) as described previously [39] ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected tissues were homogenized in RIPA buffer containing a protease inhibitor cocktail (P8340; Sigma-Aldrich), and the supernatants were collected after centrifugation at 16,000 × g for 30 m ...
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bioRxiv - Immunology 2020Quote: Cells and tissues were lysed in lysis buffer supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and protein concentration was determined using a BCA assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... COCS or brain tissues were lysed in RIPA buffer supplemented with proteinase inhibitor cocktail (Sigma, 000000011697498001). Western blotting was performed as previously described (Liu et al ...
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bioRxiv - Neuroscience 2023Quote: ... The tissues/cells were lysed by cell permeabilized buffer containing protease inhibitor (Sigma, Cat No. P8380) and phosphatase inhibitor (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...