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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant plasmids were transformed into Tuner cells (Novagen) in Luria-Bertani (LB ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Plant Biology 2022Quote: ... and anti- Rabbit IgG (whole molecule)–HRP (Sigma A0545 ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescent molecule 5-carboxytetramethylrhodamine (TAMRA, Sigma–Aldrich Japan) was used for molecular diffusion analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... All siRNA molecules were synthesized by Sigma-Aldrich. Sequences of all siRNA molecules are listed in Table S1.
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bioRxiv - Cancer Biology 2021Quote: ... Small molecule Wnt activator CHIR99021 (CHIR, Sigma, SML1046) was administered to cells in DMSO (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... The adhesion surface was previously coated with laminin 10 μg/mL (Sigma) for at least 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ECM adhesion was monitored using the Millicoat™ ECM Screening Kit (Millipore). Prior to each experiment ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used in this study were as follows: anti-mouse IgG whole molecule-Peroxidase (1:80,000; catalog no. A9044, Sigma-Aldrich) and anti-rabbit IgG whole molecule-Peroxidase (1:80,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Cutaneous melanomas were induced with topical application of 4-hydroxytamoxifen (4-HT) (Sigma) on the skin of the lower back as per protocol in [27] ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Genetics 2020Quote: ... the dish was transferred to another Nikon inverted microscope that was equipped with a CellEctor single-cell isolation system (Molecular Machines and Industries) and cell adhesion was loosened by exchanging the culture medium with a PBS-based non-enzymatic dissociation reagent (Sigma). Within ∼30 min of applying dissociation reagent ...
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bioRxiv - Biophysics 2023Quote: ... After incubation with concanavalin A to promote cell adhesion (t = 30 min, 100 µL, 0.5 mg/mL, C5275 Sigma,[89]), cells were plated onto the bottom dish surface and the cavity was covered at the top with a 22×22 cm cover glass (Ted Pella).
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bioRxiv - Molecular Biology 2021Quote: ... and 103 U/mL ESGRO recombinant mouse leukemia inhibitory factor (LIF) (Millipore; ESG1107) in an humidified cell incubator with 5% CO2 at 37°C.
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bioRxiv - Immunology 2022Quote: ... the culture medium was supplemented with 2.5ug/mL recombinant mouse EGF (Sigma-Aldrich) 16 hours before harvesting.
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bioRxiv - Cell Biology 2022Quote: ... and supplemented with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore). Cultured mESCs at 60% confluency were washed with PBS twice before the preparation of total lysate ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 hours at 37°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Cancer Biology 2019Quote: Media was conditioned with melanoma cells for 24-72 hours and concentrated 10x using Amicon Ultra 3-kDa Concentrators (Millipore Z740169). Amyloid Beta-40 ELISA was performed using the Human AB40 ELISA Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with secondary antibody in PBS (1:250) anti-Mouse IgG (whole molecule) – fluorescein isothiocyanate (FITC) antibody produced in goat (Sigma, F9006) in dark for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Plant Biology 2020Quote: ... the active molecule Acibenzolar-S-methyl (Sigma-Aldrich, Germany) was used ...
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bioRxiv - Physiology 2020Quote: ... Small molecules were dissolved in DMSO (276855, Sigma-Aldrich). kisspeptin-10 purchased from Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: ... Anti-Horse IgG (whole molecule) -FITC antibody (Sigma-Aldrich) was used.
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bioRxiv - Cell Biology 2019Quote: ... The SMYD3 small-molecule inhibitor BCI-121 (Sigma, SML1817) was dissolved in DMSO at a concentration of 20 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... and Goat anti-guinea pig IgG whole molecule (Sigma) at 1:10,000 were used ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... all small molecule ligands were obtained from Millipore Sigma or Cayman Chemical ...
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bioRxiv - Microbiology 2024Quote: ... All molecules used as standards were obtained from Sigma: ADPR (A0752) ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Microbiology 2021Quote: ... FLAG®-tagged MprF was detected both by a mouse anti-FLAG® primary antibody (Sigma-Aldrich) and goat antimouse secondary antibody (LI-COR ...