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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Proteins of the gel were then stained with Coomassie blue R-250 (Sigma-Aldrich, USA) and the molecular mass of the proteins was determined with reference to standard proteins (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2021Quote: ... 15N-labeled protein for NMR analysis utilized M9 media with 13C glucose and 15N ammonium salts (Sigma) as the sole carbon and nitrogen sources ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... CP-A-empty and CP-A-ERBB2 stable cells were generated by transfecting 1×106 CP-A cells with lentiviral particles containing either pHAGE-ERBB2 or pHAGE-empty using polybrene (EMD Millipore, TR-1003) at MOI of 1 for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... 4 ng/µl of the multiple-labeled goat anti-mouse IgG secondary antibodies (Sigma-Aldrich, SAB3701063-1) for the β-tubulin samples and 4 ng/µl of the multiple-labeled goat anti-rabbit IgG secondary antibody DOL 8.3 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... a secondary anti-rabbit or anti-mouse antibody labeled with Horse Radish Peroxidase (HRP) (NA934VS, Millipore Sigma) was added at a 1/3000 dilution in 4% milk in TBST for 30 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Immunology 2021Quote: ... with 10% FCS (Sigma-Aldrich) and 100 U/ml penicillin plus 100 mg/ml streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FCS (Sigma), 2mM L-glutamine (Thermofisher) ...
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bioRxiv - Genomics 2020Quote: ... 15% FCS (Sigma ES-009), 100 units/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... and 1% FCS (Sigma-Aldrich) three times for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FCS (Sigma), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 8% FCS (Sigma), penicillin/streptomycin (Roche ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% FCS (Sigma-Aldrich), 100U/ml penicillin and 100μg/ml puromycin (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... with 10% FCS (Sigma-Aldrich), 750 μl milk EVs or EV-depleted controls in a total volume of 1 ml and cultured for the indicated amount of time.
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bioRxiv - Immunology 2020Quote: ... with 10% FCS (Sigma-Aldrich), 750 μl milk EVs or EV-depleted controls in a total volume of 1 ml and cultured for 6 days ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... containing 2% FCS (Sigma Aldrich), 0.1 mg/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... containing 20% FCS (Sigma-Aldrich) and 1% Pen/Strep (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... containing 10% FCS (Sigma Aldrich) and plated at a density of 4 × 103 cells /cm2 ...
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bioRxiv - Bioengineering 2020Quote: ... Fluorinert FC-40 (Sigma-Aldrich), Pluronics F-127 (EMD Millipore Corp ...
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bioRxiv - Immunology 2019Quote: ... 5% (v/v) FCS (Sigma). The following day ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10 % FCS (Sigma), 100 U/ml penicillin/streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FCS (Sigma-Aldrich), 100 μg/ml gentamycin (Gibco ...