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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Mouse Cd79a protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Systems Biology 2020Quote: ... and a HRP-labeled goat anti-mouse antibody (1:10,000, Sigma-Aldrich).
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bioRxiv - Physiology 2020Quote: ... alkaline phosphatase-labeled goat antibodies against mouse IgM μ chain (Sigma-Aldrich) and mouse IgG1 γ chain (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... pH=8.5 and labeled with the mouse anti-BrdU antibody (Millipore, MAB3424) at 1:1000 dilution followed by the incubation with biotin-linked anti-mouse antibody and streptavidin-horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were labeled with anti-α-tubulin mouse antibody (clone DM1A) (Sigma), and lamin was labeled with a custom-made rabbit polyclonal antibody (Wesolowska et al. ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... and 103 U/mL ESGRO recombinant mouse leukemia inhibitory factor (LIF) (Millipore; ESG1107) in an humidified cell incubator with 5% CO2 at 37°C.
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bioRxiv - Immunology 2022Quote: ... the culture medium was supplemented with 2.5ug/mL recombinant mouse EGF (Sigma-Aldrich) 16 hours before harvesting.
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bioRxiv - Cell Biology 2022Quote: ... and supplemented with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore). Cultured mESCs at 60% confluency were washed with PBS twice before the preparation of total lysate ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 hours at 37°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma Aldrich) were used.
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bioRxiv - Neuroscience 2022Quote: ... anti-adenomatous polyposis coli clone CC1 (APC/CC-1) (1:100, mouse, Millipore Cat# OP80) anti-platelet derived growth factor receptor alpha (PDGFRa ...
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bioRxiv - Immunology 2020Quote: ... FCS (Sigma Life Science ...
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Microbiology 2023Quote: ... G9R and A1L proteins were labeled with primary anti-Flag (F1804, Sigma-Aldrich) and anti-HA (26183 ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... The FLAG-tagged PCRK2 and PBL19 proteins were immunoprecipitated using 15 μl M2 beads (Sigma; Cat. #A2220). Biotinylation was detected with Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged NRG1C and SAG101 proteins were immunoprecipitated using 20 µl M2 beads (Sigma; Cat. #A2220) for co-immunoprecipitation and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Microbiology 2020Quote: ... HA3-tagged proteins were detected using a monoclonal rat α-HA3 (1:1000; clone 3F10; Sigma-Aldrich). Actin ...
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bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of Flag-tagged proteins were carried out with the anti-FLAG M2 beads (Cat. A2220, Sigma) in RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged proteins were incubated with 30 μl pre-washed anti-FLAG M2 affinity gel (Sigma, #A2220) for 3 h at 4 °C and washed four times with IP wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...