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Citations for Millipore Sigma :
101 - 150 of 4082 citations for Recombinant Human VCAM1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... ErbB2 (Her2) human recombinant was purchased from Sigma-Aldrich. Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... 10 nM recombinant human [Leu15]-gastrin I (Sigma G9145), 50 ng/ml recombinant mouse EGF (Peprotech ...
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bioRxiv - Genomics 2019Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and 1% penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Genomics 2020Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant human IGF1 (Sigma Chemicals, St. Louis, MO, USA), or glucose.
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Genetics 2023Quote: ... 10 μg/ml recombinant human insulin (Sigma, 19278-5ML), 2 IU/ml heparin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant insulin at 0.01 mg/mL (Sigma, I9278), and 1% penicillin-streptomycin mix ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human (rh) Interleukin-2 (IL-2, Sigma-Aldrich), rhTNFα (Peprotech ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mg/ml human recombinant albumin (Sigma Aldrich, A9731) and 0.2 mg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2.5 μg/ml human recombinant insulin (Sigma, 19278). Wide-bore P1000 pipette tips with ∼3 mm diameter openings were used to transfer tumor pieces ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant human chorionic gonadotropin (hCG) (Sigma), 500 ng/ml of A4 (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 nM recombinant human (Leu15)-gastrin I (Sigma; G9145), 50 ng/mL recombinant human EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.8% NaCl in 1X PBS) containing primary antibodies (VCAM1 Millipore Sigma MAB1398Z 1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... and 1.8% NaCl in 1X PBS) containing primary antibodies (VCAM1 Millipore Sigma MAB1398Z 1:200 ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... and brain-derived neurotrophic factor (BDNF, human recombinant, Sigma-Aldrich). Concentrations used were in the range 50-100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37° C for 3 days in a humidified CO2 incubator.
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bioRxiv - Biophysics 2020Quote: ... 100 nM recombinant human EGF (Sigma-Aldrich, St. Luis, MS). For LatA treatments 1 µM LatA was added to cells and incubated for 10 min at 37°C prior to imaging ...
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bioRxiv - Immunology 2019Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator.
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bioRxiv - Physiology 2020Quote: ... 5 μg/mL recombinant human insulin (Sigma-Aldrich, Dorset, UK), 0.5 ng/mL recombinant human basic fibroblast growth factor ...
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bioRxiv - Immunology 2020Quote: ... 300 U/mL Roche recombinant human IL-2 (Sigma-Aldrich) and 1 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Immunology 2020Quote: ... and recombinant human RAP (Merck Millipore, 200 nM final concentration) were used to stimulate nuclear translocation of MerTK.
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml human recombinant epidermal growth factor (Sigma, UK) and 25 μg/ml fetuin from FBS (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng/ml of recombinant human bFGF (F0291, Sigma-Aldrich, 50 μg/mL stock solution in 20 mM Tris pH=7.5 + BSA 0.1 % ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg/mL human recombinant insulin (Sigma-Aldrich, 91077C).
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL recombinant human insulin (Sigma Aldrich #I9278-5ml), and 1% penicillin-streptomycin (Thermo Scientific #15140122) ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA for SBP-tagged human FKBP12.6 was subcloned into the pET28 vector (Novagen) containing a 6×His-tag at the N-terminus ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...