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Citations for Millipore Sigma :
451 - 500 of 9872 citations for Recombinant Human TES Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Cell Biology 2019Quote: ... The remaining buffer was removed and the cells were resuspended in 0.8 mL of cold TE buffer (Tris-HCl, pH 7.5, EDTA) with protease inhibitors (aprotinin, pepstatin, leupeptin (Sigma)) and 10 μL was boiled directly in 90 μL of 2x sample buffer (lysate) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The DNA was then purified from digested agarose using drop dialysis against TE buffer on a 0.1µm dialysis membrane (Millipore) for 2.5 h.
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bioRxiv - Cell Biology 2021Quote: ... The remaining buffer was removed and the cells were resuspended in 0.8 mL of cold TE buffer (Tris-HCl, pH 7.5, EDTA) with protease inhibitors (aprotinin, pepstatin, leupeptin (Sigma)) and 10 μL was boiled directly in 90 μL of 2x sample buffer (lysate) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was eluted from the beads in 50 μl 1x TE buffer (pH 8, Sigma Aldrich, MO, USA) at 35°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: Eluted products were pooled to 500 μL in TES and incubated with 1 mg/ml Proteinase K (Sigma) for 30 min at 60 °C ...
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bioRxiv - Genomics 2019Quote: ... were resuspended to a final volume of 200 nM in TE pH 8.0 (Sigma-Aldrich, Saint Louis, MO). Libraries were amplified using 1 μL of a 10-fold dilution of the library ...
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bioRxiv - Immunology 2021Quote: Each sample contained three flies that were homogenised in 75μl of TE + 0.1% Triton X-100 (Sigma Aldrich). Samples were incubated for 20 min at 75°C and stored at −80°C ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant plasmids were transformed into TUNER (Novagen) cells in LB broth containing 10 μg/ml kanamycin at 37 °C shaking at 180 rpm ...
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bioRxiv - Immunology 2022Quote: ... DNAse I recombinant (Roche by Sigma Aldrich) and sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 μg/l recombinant mouse (Sigma-Aldrich) or human epithelial growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant 6His-ErbB3 was purchased from Sigma. The in vitro binding assay was conducted by incubating 1 μg/ml recombinant ErbB3 with 1 μg/ml recombinant Rabaptin5 in a stringent buffer containing 50 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... 103 IU recombinant mouse LIF (Millipore, ESG1107), 2mM L-glutamine (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... a recombinant HRV 3C protease (Sigma-Aldrich) was added to NSP7/NSP8 complex and incubated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet was resuspended in TE buffer with 20 mg/ml of lysozyme (Sigma-Aldrich, CAT No.:L7651) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: Chromatin was isolated from supernatant by adding 3 volumes of TE with 1% SDS and RNAse A (Sigma R6513), and vortexing to mix ...
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bioRxiv - Genomics 2021Quote: Isolates were cultured in CFA broth overnight at 37°C followed by cell suspension in TE buffer (10 mM Tris and 1 mM EDTA pH 8.0) with 25% sucrose (Sigma) followed by lysis using 10 mg/ml lysozyme (in 0.25 Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2019Quote: ... Flies were starved for 1 hr on 1% agar supplemented with 2% phosphate buffered saline (PBS) at 29° before being manually smashed in 75μl TE + 0.1% Triton X-100 (Sigma Aldrich). 3 flies per sample were used ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The cells were re-suspended in 50 ml of TE (10 mM Tris-HCl pH 8.0, 1 mM EDTA) that contained chicken lysozyme (1 mg/ml, Sigma) and DTT (10 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Pellets were resuspended in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0; Sigma-Aldrich) and reverse-crosslinked ...
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Disparate regulation of imd drives sex differences in infection pathology in Drosophila melanogasterbioRxiv - Immunology 2020Quote: Each sample consisted of 3 flies that were homogenised in 75μl of TE + 0.1% Triton X-100 (Sigma Aldrich). Samples were incubated for 20 min at 75°C and stored at −80°C ...
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bioRxiv - Microbiology 2022Quote: ... samples were re-suspended in 50 μl lysis buffer (47.5 μl TES-Sucrose and 2.5 μl lysozyme [10 μg ml-1]; Sigma-Aldrich) and incubated at 37°C for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were thawed on ice and resuspended in 600 µL each of TES (10 mM Tris pH 7.5, 10 mM EDTA, 0.5% SDS) buffer and acid phenol (Sigma P4682) and shaken at 1,400 rpm in a Thermomixer® R (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: The dorsal flank skin of mice was sampled using a swab pre-wetted in a TES solution containing 1.2% Triton X-100 (Sigma) vigorously rubbed on the skin surface ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were treated with RNase A (345 μL TE, 3 μL 10 mg/mL RNAse A (Sigma-Aldrich, R6513), 2 μL 20 mg/mL Glycogen (Roche ...
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bioRxiv - Genomics 2023Quote: ... The pellet was then resuspended in 1 ml of 1x TE + complete protease inhibitor cocktail (EDTA-free, Sigma Aldrich) and transferred to a 2 ml Eppendorf tube with 4 µl of Ready to lyse Lysozyme for 20mn at room temperature ...
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bioRxiv - Cell Biology 2023Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: Lyophilised and high-performance liquid chromatography (HPLC)-purified oligonucleotides (Eurofins Genomics) were resuspended in Tris-EDTA (TE) buffer (pH 8.0, Sigma-Aldrich) at a concentration of 100 µM ...