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Citations for Millipore Sigma :
1 - 50 of 4020 citations for Recombinant Human Metadherin GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... and/or 0.4μg human GST-tagged PP2A-α (Sigma, SRP5336) in the presence or absence of AZ’5576 or okadaic acid (Abcam ab120375 ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Microbiology 2024Quote: Recombinant untagged Ssp4 and His-GST tagged Ssp6 (prepared as below) were coupled to cyanogen bromide activated agarose (Sigma Aldrich) based on the method described in57 ...
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST-tag (Step 2 ...
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bioRxiv - Microbiology 2024Quote: ... GST and 3×FLAG-tagged proteins were detected with a rabbit polyclonal anti-GST antibody (1:2500, Sigma-Aldrich) and a mouse monoclonal HRP-conjugated anti-FLAG antibody (M2 1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant human (AG968, Sigma). The information for all other reagents is indicated in corresponding sections.
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Developmental Biology 2022Quote: ... GST and GST-Rab11DN were purified from the supernatant with Glutathione Sepharose 4B GST-tagged protein purification resin (GE17-0756-01, Sigma-Aldrich) using batch method ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... and GST-tagged proteins were purified using pre-hydrated glutathione agarose beads (Sigma Aldrich). His6-tagged proteins were eluted by sequential washes with imidazole-containing buffers at increasing concentrations (10 mM ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant human PDE8A (Sigma SRP0273) was incubated with 100 ng FAK tyrosine kinase (Promega #V1971 ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Neuroscience 2022Quote: ... and 1μM recombinant human insulin (Sigma). Organoids were collected from the wells on day 14 and transferred to 10cm dishes at roughly 20 organoids per dish ...
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bioRxiv - Physiology 2022Quote: ... 500μg/ml human recombinant albumin (Sigma), 213μg/ml L-ascorbic acid (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... human recombinant TNF (H8916, Sigma-Aldrich) or chemical inhibitors (SB203580 from Selleck ...
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bioRxiv - Developmental Biology 2024Quote: ... 10nM recombinant human gastrin (Sigma; G9145), 5ng/mL recombinant human HGF (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant human TNFα (Sigma Aldrich, #H8916), PGE2 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: GST-tagged Af1521 macrodomain was produced in-house and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as described previously (Hendriks et al. ...
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bioRxiv - Biophysics 2021Quote: PAI-1 (human recombinant, Sigma-Aldrich, Germany)
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bioRxiv - Biophysics 2021Quote: ... PAI-1 (human recombinant, Sigma-Aldrich, Germany), Plasminogen (human plasma purified protein ...
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bioRxiv - Cell Biology 2020Quote: ... sativa–derived recombinant human albumin (Sigma-Aldrich), and 213μg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10μM recombinant human Gastrin (Sigma-Aldrich G9145), 50ng/mL recombinant human HGF (Peprotech 100-39H) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant Human Insulin (Sigma cat no. I9278) 0.02mg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... 0.002 mg/mL recombinant human insulin (Sigma), 0.1% Trace Elements A (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Bioengineering 2021Quote: ... 0.002 mg/ml recombinant human insulin (Sigma), 0.1% Trace Elements A (Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... 0.5% recombinant human serum albumin (A9986, Sigma), 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ng of recombinant human AChE (Sigma) or 30 μg of tissue lysate was incubated with steroidal alkaloid compound or at room temperature for 30 minutes in 50 mM Tris pH 8.0 in a total volume of 90 μL ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant human IFN-α2a (H6041; Sigma-Aldrich) was reconstituted in PBS with 0.1% BSA and added to the basolateral pole at a final concentration of 100 U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant 2N4R human Tau (Tau441, AG960, Millipore) at serial dilutions ranging from 20 µg/ml to 0 µg/ml total tau was used to generate a calibration curve and calculate total tau in our samples.
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bioRxiv - Cell Biology 2022Quote: ... 0.025-μg·ml-1 recombinant human EGF (Sigma), 0.1-μg·ml-1 cholera toxin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... 10 μg/ml recombinant human insulin (Sigma), 2 IU/ml heparin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL human recombinant bFGF (Sigma), and 10 μM Y-27632 (STEMCELL Technologies)) ...