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Citations for Millipore Sigma :
601 - 650 of 4382 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... we used 100 μM picrotoxin (an antagonist of GABAA and GABAC receptors; Sigma). To block glycine receptors ...
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bioRxiv - Neuroscience 2022Quote: ... we used 5 μM SR-95531 (gabazine, an antagonist of GABAA receptors; Sigma). To block both GABAA and GABAC receptors ...
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bioRxiv - Neuroscience 2020Quote: ... The GABAA receptor agonist muscimol (1 mg/mL, Sigma-Aldrich, St. Louis, MO) or vehicle (0.9% sterile saline ...
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bioRxiv - Neuroscience 2021Quote: ... and the N-methyl-D-aspartate receptor agonist D-Glutamate (0.75mM, Sigma-Aldrich) was bath applied to the spinal cord.
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular solution containing the opioid receptor antagonist naloxone (300 µM, Sigma Aldrich, Germany) was perfused to the MOB slice for at least 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... the D2 DA receptor (D2R) agonist quinpirole (20 μM, Sigma, St. Louis, MO) was applied at the end of each experiment as a pharmacological test of DA neuron ientity.25 Whole-cell somatodendritic nAChR-mediated currents were evoked in the presence of tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2020Quote: ... mouse (IgG2a) anti-GluA2 (Glutamate receptor subunit A2) at 1:2000 (#MAB397, Millipore) for labeling post-synaptic receptor patches ...
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bioRxiv - Neuroscience 2020Quote: ... and the β-adrenergic receptor antagonist DL-propranolol (5 mg/kg; Sigma-Aldrich), immediately prior to sleep latency testing ...
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bioRxiv - Neuroscience 2019Quote: ... and GABA receptors was measured by application of 100 μM glutamic acid (Sigma) or 3 μM GABA (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and glutamate receptors (NBQX, 10 μM, Tocris; and DL-AP5, 30 μM, Sigma).
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bioRxiv - Cell Biology 2022Quote: ... a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore, catalog #: 05-474), or a mouse anti-His antibody.
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bioRxiv - Neuroscience 2022Quote: ... the non-competitive NMDA receptor antagonist MK801 (Sigma-Aldrich, St. Louis, MO, USA) was administered in a separate cohort (0.3 mg/kg ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Physiology 2022Quote: Drugs used throughout studies include MSX-3 (A2A receptor antagonist; #M3568; Millipore Sigma), ketanserin tartrate (5-HT2A/C receptor antagonist ...
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bioRxiv - Neuroscience 2023Quote: The non-selective muscarinic receptor antagonist scopolamine hydrobromide (SCOP; Sigma Aldrich, Ontario, Canada) was dissolved in 0.9% physiological saline and given ip at 0.3 mg/kg based on past evidence that scopolamine successfully blocks destabilization at this dose (Stiver et al. ...
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bioRxiv - Neuroscience 2023Quote: ... GluR2/3: Anti-Glutamate Receptor 2 & 3 antibody (1:500, Millipore, Cat# AB1506). c-Fos ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and muscarinic acetylcholine receptor antagonist atropine (5 mg kg−1, Y000878, Sigma-Aldrich) were injected intraperitoneally (i,p.)27 ...
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bioRxiv - Cell Biology 2024Quote: ... the EP2 receptor antagonist AH6809 (10μM in DMSO, day 0-42; Sigma-Aldrich) and the EP4 receptor antagonist AH23848 (10μM in DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... the GABA-A receptor antagonist gabazine (SR-95531, Sigma Aldrich,St. Louis, MO) was applied bilaterally (0.005mM-0.01mM in PBS (Carl Roth ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant plasmids were transformed into TUNER (Novagen) cells in LB broth containing 10 μg/ml kanamycin at 37 °C shaking at 180 rpm ...
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bioRxiv - Immunology 2022Quote: ... DNAse I recombinant (Roche by Sigma Aldrich) and sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 μg/l recombinant mouse (Sigma-Aldrich) or human epithelial growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant 6His-ErbB3 was purchased from Sigma. The in vitro binding assay was conducted by incubating 1 μg/ml recombinant ErbB3 with 1 μg/ml recombinant Rabaptin5 in a stringent buffer containing 50 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... 103 IU recombinant mouse LIF (Millipore, ESG1107), 2mM L-glutamine (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... a recombinant HRV 3C protease (Sigma-Aldrich) was added to NSP7/NSP8 complex and incubated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...