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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Human LIF Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng/ml of recombinant human bFGF (F0291, Sigma-Aldrich, 50 μg/mL stock solution in 20 mM Tris pH=7.5 + BSA 0.1 % ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg/mL human recombinant insulin (Sigma-Aldrich, 91077C).
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL recombinant human insulin (Sigma Aldrich #I9278-5ml), and 1% penicillin-streptomycin (Thermo Scientific #15140122) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with recombinant protein (GenScript, Sigma, Tocris) of each analyte for 4 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant proteins of Ubiquitin (10µg) (Sigma-Aldrich, U6253) or human His6-ubiquitin (Boston Biochem ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SLAMF7 protein (r-SLAMF7) was from Sigma. Purified anti-SLAMF7 (162.1 ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant IGF-I protein at 10nM (Sigma, #I3769), recombinant IGF-II protein at 10nM (R&D ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA for SBP-tagged human FKBP12.6 was subcloned into the pET28 vector (Novagen) containing a 6×His-tag at the N-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human epidermal growth factor (20 ng/mL, Sigma-Aldrich). Cell authentication by Ipatimup’s Cell Lines Bank ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblast Growth Factor basic human recombinant animal-free (GF003AF; Sigma Aldrich); hEGF Epidermal Growth Factor Human (E9644 ...
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bioRxiv - Genomics 2022Quote: ... Recombinant human insulin (0.75 IU insulin/kg BW) (Sigma-Aldrich, I9278) was then administered intraperitoneally ...
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bioRxiv - Immunology 2019Quote: ... and recombinant human interleukin-2 (rhIL-2; 25U/ml; Sigma-Aldrich). To mimic a transient stimulation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... baseline blood glucose was taken before recombinant human insulin (Sigma, USA) (0.5 U/kg – 1 U/kg ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant human MMP-3 (SRP7783) was purchased from Sigma Aldrich (Germany).
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bioRxiv - Cell Biology 2022Quote: ... 20 ng/ml recombinant human epidermal growth factor (hEGF, #E9644, Sigma), 0.25 ng/mL hydrocortisone (#H0888 ...
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bioRxiv - Bioengineering 2022Quote: ... and recombinant insulin human (≥ 98%) were all purchased from Sigma-Aldrich. Water (LC-MS grade) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant human Hepatocyte Growth Factor (HGF) was obtained from Sigma Aldrich Israel ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg/ml of recombinant human insulin (Sigma-Aldrich, 19278-5ML), 2 IU/ml heparin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and 100 μl LIF(Millipore, LIF1010). Spheroids were cultured for 3 weeks in 25 cm2 flask (Greiner ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1000 U/ml LIF (Millipore). HEK293T cells were cultured in DMEM supplemented with 10% FBS and maintained at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2020Quote: ... 1000 U/mL LIF (Millipore ESG1107), 3uM CHIR99021 (Millipore 361559 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1000 units/mL LIF (ESGRO, Sigma), 3 μM CHIR99021 (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1000 U/mL LIF (ESG1107 Millipore), 3 µM CHIR99021 (LC Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1000 U/mL LIF (ESG1107 Millipore), sterilized using 0.1 µm filter flask (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:10000 LIF (Millipore ESG1106). Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
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bioRxiv - Genomics 2020Quote: ... and 107 U/ml LIF (Millipore).
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... 1,000 U/ml LIF (Millipore, #LIF1005) and 10 μM of Y-27632 to be induced into hPGCLCs.
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bioRxiv - Molecular Biology 2019Quote: ... and leukemia inhibitory factor (LIF) (Millipore), 2i/Serum condition ...