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Citations for Millipore Sigma :
351 - 400 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Neuroscience 2022Quote: D2 receptor-mediated outward currents were recorded in the presence of the following receptor antagonists (all from Sigma-Aldrich): MK-801 (30 μM added to the recovery bath ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... PLA2G15 used in the reactions was obtained by overexpressing C-terminally FLAG-tagged human PLA2G15 in HEK293FT cells and affinity-isolation using anti-FLAG magnetic beads (Sigma-Aldrich #M8823).
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Physiology 2019Quote: ... and insulin receptor β-subunit (Millipore 07-724). Plasmids to express flag-tagged PPARγ ...
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bioRxiv - Neuroscience 2020Quote: ... a GABAA receptor blocker (bicuculline 10 μM, Sigma), NMDA receptor blockers (D-AP5 50 μM ...
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bioRxiv - Neuroscience 2020Quote: ... anti-rabbit dopamine D2 receptor (cat# AB5084P, Millipore), anti-rabbit AMPA receptor (GluR1 ...
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bioRxiv - Neuroscience 2022Quote: ... GABAA receptor antagonist picrotoxin (PTX, P1675, Sigma-Aldrich), PKI (6221 ...
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bioRxiv - Neuroscience 2019Quote: ... we injected the GABAA receptor agonist muscimol (Sigma; 8.8 nmol [1µg] or 44 nmol [5 µg] in a 1 µl volume ...
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bioRxiv - Cancer Biology 2021Quote: ... or DMH1 (BMP receptor, Sigma #D8946, 0.5 µM) or EC330 (LIF receptor ...
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bioRxiv - Cancer Biology 2022Quote: ... or the thrombin receptor-activating peptide (TRAP, Sigma) was added to each sample and incubated at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the GABAB receptor antagonist phaclofen (Sigma, USA) were dissolved in saline and administered at doses of 0.06 nmol/0.2 μL/mouse32 and 0.1nmol /0.2μL/ mouse33 ...
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bioRxiv - Neuroscience 2023Quote: ... M2 muscarinic receptor (rat anti-M2, Millipore, MAB367), Parvalbumin (PV ...
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bioRxiv - Neuroscience 2023Quote: ... GABAA receptor antagonist bicuculline methiodide (#14343; Millipore Sigma) and gabazine (#1262 ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Neuroscience 2022Quote: ... 6-cyano-7-nitroquinoxaline-2,3-dione-disodium salt (CNQX, a non-NMDA receptor blocker), and picrotoxin (PIC, a GABAA receptor blocker) were purchased from Sigma-Aldrich, and L-2-amino-5-phosphonopentanoic acid (APV ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Neuroscience 2021Quote: The nicotinic acetylcholine receptor (nAChR) blocker mecamylamine and GABAA receptor blocker picrotoxin (PTX) were obtained from Sigma Aldrich (Cat#: M9020, P1675 respectively). The voltage-gated sodium channel blocker tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were incubated with guinea pig antibody to D1 receptors (1:400; Alomone, Jerusalem, Israel) and rabbit antibody to D2 receptors (1:500; Millipore, Burlington, MA), washed ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... Sunitinib malate (Receptor tyrosine kinase inhibitor, 0.05µM Sigma-Aldrich).
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... and the retinoic receptor agonist TTNPB (RA; Sigma Aldrich). Day-specific differentiation media formulations were as follows:
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bioRxiv - Genomics 2020Quote: ... and the retinoic receptor agonist TTNPB (RA; Sigma Aldrich). Day-specific differentiation media formulations were as follows:
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bioRxiv - Neuroscience 2019Quote: ... phasic GABAA receptors with SR95531 (0.5 µM; Sigma, Australia), and extrasynaptic (tonic ...
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bioRxiv - Neuroscience 2019Quote: ... the GABAA receptor antagonist gabazine (5μM, Sigma cat#S106) was bath applied and if a GABAB receptor response remained ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µM strychnine hydrochloride (glycine receptor antagonist, Millipore Sigma), 10 µM NBQX disodium salt (AMPA receptor antagonist ...
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bioRxiv - Neuroscience 2020Quote: ... anti-rabbit dopamine D(1A) receptor (cat# ABN20, Millipore) overnight ...