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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Recombinant Human Interleukin 2 Receptor Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-alpha Smooth Muscle Actin-FITC (Sigma, F3777). Secondary antibodies used include goat anti-rabbit FITC ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Genetics 2019Quote: FLAG-tagged proteins were immunoprecipitated from 2 mg of HEK293T or M17 cells using anti-FLAG M2 affinity gel (Sigma A2220). Lysates were prepared in NP40 buffer (see Western blotting) ...
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-mouse FITC tagged (1:200, Sigma) at room temperature in dark for 90 min ...
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bioRxiv - Neuroscience 2020Quote: ... GABA-A receptor antagonist and Bicuculline (Bic) (Sigma, 285269, USA), were dissolved in CSF tracer before use at a concentration of 0.2 mM ...
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bioRxiv - Neuroscience 2019Quote: ... the GABAB receptor antagonist CGP 55845 (5μM, Sigma cat#SML0594) was added to the bath ...
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bioRxiv - Bioengineering 2021Quote: ... rat monoclonal anti-Nicotinic Acetylcholine Receptor (Sigma-Aldrich, 1:800). The following secondary antibodies (diluted in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the glutamate receptor antagonist kynurenic acid (4 mM; Sigma– Aldrich) was locally applied in the pretectum by pressure injection through a micropipette fixed to a holder (containing Fast Green to aid visualization of the injection spread) ...
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bioRxiv - Neuroscience 2020Quote: ... an AMPA and kainate receptor blocker (NBQX 10 μM, Sigma), and an inwardly rectifying potassium channel blocker (barium chloride 200 μM ...
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bioRxiv - Neuroscience 2021Quote: ... and D2-like dopamine receptors (Sulpiride, Sulp; 10 μM, Sigma, substances were applied for at least 15 min prior STDP recordings) ...
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bioRxiv - Neuroscience 2020Quote: Mecamylamine hydrochloride (nicotinic receptor antagonist; Sigma (St. Louis, MO, USA)) was applied directly at the surface of the dorsal horn using the agar chamber described above ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit anti-Opioid Receptor-Mu (Millipore, AB5511, 1:500). The secondary antibodies were donkey anti-rabbit IgG conjugated with Alexa-488 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The TRPV1 receptor agonist capsaicin (3 μM; Sigma-Aldrich, M2028) was applied at the end of experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... using bonafide GABA-A receptor agonists like GABA (Sigma #A2129), Muscimol (Sigma #M1523) ...
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bioRxiv - Neuroscience 2022Quote: ... adrenergic α1B receptor antagonist – Chloroethylclonidine (30 μM, Sigma-Aldrich B003); and adrenergic α1D receptors antagonist – BMY 7378 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: ... a GABAA-receptor antagonist (Sigma−Aldrich, St. Louis, MO, USA) was used ...
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bioRxiv - Neuroscience 2022Quote: ... and strychnine hydrochloride (Strych) (glycine receptor antagonist, 0.3µM, Sigma, #S8753) dissolved in Ringer’s solution ...
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bioRxiv - Molecular Biology 2022Quote: ... antibodies against Epidermal Growth Factor Receptor (EGFR) (Millipore, 06-847) were used at 1:200 dilution at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... and the EP2 receptor inhibitor AH6808 at 10µM (Sigma-Aldrich). After washing with PBS-/- ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µM fibroblast growth factor receptor inhibitor (FGFR, Sigma-Aldrich) and 3 µM GSK inhibitor (CHIR99021 ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500 anti-Glutamate receptor AMPA R4 (#G5790, Sigma-Aldrich). Fluorescent secondary antibodies (1:10000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All sera were treated with receptor destroying enzyme (RDE; Sigma) for 16-18 hours to reduce non-specific antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... L-741,626 (10 mg/kg, D2 receptor antagonist) (Sigma-Aldrich). Doses selected were based on previous studies and pilot experiments to control for confounding effects such as motor impairment (Lustberg et al 2020a ...
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bioRxiv - Neuroscience 2024Quote: ... flupenthixol (0.25 mg/kg, nonspecific DA receptor antagonist) (Sigma-Aldrich); SCH-23390 (0.03 ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... and a baseline optical signature was recorded before adding 10 μL of the cannabinoid receptor agonist WIN-55,212-2 (Sigma-Aldrich, 100 nM final concentration) dissolved in assay buffer (HBSS with 20 mM Hepes ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1 M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial cells (2 × 106 CFU) were resuspended in nonspecific human IgG (Sigma, UK) for one hour on ice ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 2 μm latex green beads were incubated with human IgM (I-8260, Sigma), then in rabbit anti-human IgM (Sigma 270A ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...